前列腺素 E2 酶联免疫试剂盒 PGE2 ELISA kit

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前列腺素 E2 酶联免疫试剂盒 PGE2 ELISA kit前列腺素 E2 酶联免疫试剂盒

PGE2 ELISA kit

 

　　用于炎症和类花生酸研究的高灵敏度前列腺素E2酶联免疫试剂盒。

 

　　研究背景：前列腺素 E2（PGE2）由于其在炎症、癌症、动脉粥样硬化、自身免疫性疾病和脓毒症中具有重要地位而被广泛研究。氧化的花生四烯酸通过前列腺素合成酶（COX-1和COX-2）生成前列腺素 H2（PGH2），被前列腺素合成酶进一步代谢成主要产物前列腺素 E2（PGE2）。前列腺素 E2（PGE2）通过与细胞表面的 G-蛋白偶联受体（EP1、EP2、EP3 和 EP4）的结合来介导自分泌和旁分泌的信号传导，从而调节磷脂酶Ｃ和腺苷酸环化酶活性。PGE2 作为一种治疗靶点，无论是通过 COX 抑制剂（NSAIDS）调节其合成，还是通过下调其与受体结合或结合拮抗剂，都引起了人们极大的兴趣。在各种组织中产生的前列腺素 E2（PGE2）已在多种生理过程中表现出了调节作用，包括在肾脏中尿钠排泄，血管中平滑肌的弹性，以及单核细胞和巨噬细胞对损伤组织的炎症作用。

 


◆产品特点


●　高灵敏度测定，可检测最少 13.4 pg/mL 的前列腺素 E2

●　3小时内即可获得多达37个样本重复检测的高通量格式的结果

●　广泛引用于同行评审文献中

●　准备使用的液体颜色编码试剂以便减少错误发生

●　每次使用和不同批次产品获得良好重复性的结果

 

前列腺素 E2 酶联免疫试剂盒 PGE2 ELISA kit 前列腺素 E2 酶联免疫试剂盒 PGE2 ELISA kit

 

    

[1]

Colon cancer-derived myofibroblasts increase   endothelial cell migration by glucocorticoid-sensitive secretion of a   pro-migratory factor: Z. Drebert, et al.; Vascul. Pharmacol. 89, 19 (2017), Application(s): Determine the concentrations of   prostanoids in conditioned medium from myofibroblasts and HUVECs, and in   HUVEC lysates, Abstract;

[2]

Expression of toll-like receptor-1 by bradykinin in   human gingival fibroblasts: J.A. Arreguin-Cano T; Int. J. Clin. Exp. Pathol. 10, 1283 (2017), Application(s): Measurement of PGE2 in   supernatant., Full Text

[3]

Human and feline   adipose-derived mesenchymal stem cells have comparable phenotype,   immunomodulatory functions, and transcriptome: H.C. Clark, et al.; Stem Cell   Res. Ther. 8, 69 (2017), Application(s): Measurement of PGE2   in supernatant of human and feline cells., Abstract; Full Text

[4]

Novel augmentation   by bufalin of protein kinase C-induced cyclooxygenase-2 and IL-8 production   in human breast cancer cells: H.T. Chen, et al.; Innate Immun. 23, 54 (2017), Abstract; Full   Text

[5]

Prostaglandin E2 stimulates the expression of cumulus   expansion-related genes in pigs: the role of protein kinase B: M. Blaha, et   al.; Prostaglandins Other Lipid Mediat. 130, 38 (2017),   Application(s): ELISA using pig samples, Abstract;

[6]

A positive feedback loop between progesterone and   microsomal prostaglandin E synthase-1-mediated PGE2 promotes production of   both in mouse granulosa cells: K. Tamura, et al.; Prostaglandins Other Lipid   Mediat. 123, 56 (2016), Application(s): Measurement of   PGE2 in the culture media, Abstract;

[7]

Anti-inflammatory effects of 6′-O-acetyl mangiferin   from Iris rossii Baker via NF-κb signal blocking in   lipopolysaccharide-stimulated RAW 264.7 cells: J.H. Jang, et al.; Chem. Biol.   Interact. 2797, 30303 (2016), Application(s):   Determination of PGE2 production, Abstract;

[8]

Dimethyl fumarate activates the prostaglandin EP2   receptor and stimulates cAMP signaling in human peripheral blood mononuclear   cells: S.E. Fiedler, et al.; Biochem. Biophys. Res. Commun. 475, 19 (2016), Application(s): Supernatant PGE2 was measured,   Abstract;

[9]

Directly interact with Keap1 and LPS is involved in the   anti-inflammatory mechanisms of (-)-epicatechin-3-gallate in LPS-induced   macrophages and endotoxemia: Y.S. Chiou, et al.; Free. Radic. Biol. Med. 94, 1 (2016), Application(s): Measurement of prostaglandin E2   (PGE2) level in culture medium, Abstract;

[10]

Effects of Almond-   and Olive Oil-Based Docosahexaenoic- and Vitamin E-Enriched Beverage Dietary   Supplementation on Inflammation Associated to Exercise and Age: X. Capo, et   al.; Nutrients 8, 619 (2016), Application(s): Cytokine,   Eicosanoids and Adhesion Molecules Determination, plasma, Abstract; Full   Text

[11]

Enhanced   anti-inflammatory activity of brown seaweed Laminaria japonica by   fermentation using Bacillus subtilis: H.T.V. Lin, et al.; Process Biochem.   (2016), Application(s):   Measurement of prostaglandin E2 (PAGE2), cell supernatant,

[12]

Grid-like surface structures in thermoplastic   polyurethane induce anti-inflammatory and anti-fibrotic processes in bone   marrow-derived mesenchymal stem cells: Y. Roger, et al.; Colloids Surf. B   Biointerfaces 148, 104 (2016), Application(s): ELISA   using human bone marrow-derived mesenchymal stem cell supernatant, Abstract;

[13]

Implantation and   pregnancy outcome of Sprague-Dawley rats fed with low and high salt diet:   G.O. Oludare, et al.; Middle East Fertil. Soc. J. (2016), Application(s): Measured rat serum PGE2 levels,

[14]

In vitro bioactive   properties of phlorotannins recovered from hydrothermal treatment of   Sargassum muticum: M.P. Casas, et al.; Sep. Purif. Technol. 167, 117 (2016), Application(s): Determination of the   amount of PGE2 in thawed plasma supernatants,

[15]

Isolation and   Identification of a Flavone Apigenin from Marine Red Alga Acanthophora   spicifera with Antinociceptive and Anti-Inflammatory Activities: G.A. El   Shoubaky, et al.; J. Exp. Neurosci. 10, 21 (2016), Application(s): TNF-α measured in mouse blood samples, Abstract; Full   Text

[16]

Local   intra-uterine Ang-(1-7) infusion attenuates PGE2 and 6-keto PGF1α in   decidualized uterus of pseudopregnant rats: K.B. Brosnihan, et al.; Reprod.   Biol. Endocrinol. 14, 68   (2016), Application(s):   Assaying tissue extracts, Abstract; Full Text

[17]

MRI-Based   Assessment of Intralesional Delivery of Bone Marrow-Derived Mesenchymal Stem   Cells in a Model of Equine Tendonitis: A. Scharf, et al.; Stem Cells Int. 2016, 1 (2016), Application(s): Assessing the   ability of MSCs to modulate the inflammatory response, Abstract; Full   Text

[18]

Resokaempferol-mediated anti-inflammatory effects on   activated macrophages via the inhibition of JAK2/STAT3, NF-κB and JNK/p38   MAPK signaling pathways: Q. Yu, et al.; Int. Immunopharmacol. 38, 104 (2016), Application(s): PGE2 was measured in mouse   supernatant, Abstract;

[19]

The Prostaglandin   E2-EP3 Receptor Axis Regulates Anaplasma phagocytophilum-Mediated NLRC4   Inflammasome Activation: X. Wang, et al.; PLoS One 12, e1005803 (2016), Application(s): PGE2 measurement in cell lysates, Abstract; Full Text

[20]

Antagonizing   Effects of Aspartic Acid against Ultraviolet A-Induced Downregulation of the   Stemness of Human Adipose Tissue-Derived Mesenchymal Stem Cells: K. Jung, et   al.; PLoS One 10, e0124417   (2015), Application(s):   ELISA using human culture supernatant , Abstract; Full Text

[21]

Anti-inflammatory effect of desoxo-narchinol-A isolated   from Nardostachys jatamansi against lipopolysaccharide: J.Y. Shin, et al.;   Int. Immunopharmacol. 29, 730 (2015), Application(s):   PGE2 level was measured by ELISA in mice, Abstract;

[22]

Effectiveness of   the combinational treatment of Laminaria japonica and Cistanche tubulosa   extracts in hair growth: K. Shin, et al.; Lab. Anim. Res. 31, 24 (2015), Application(s): ELISA using mouse exudate, Abstract; Full Text

[23]

Insight into the molecular mechanism of an herbal   injection by integrating network pharmacology and in vitro: Y.M. Ma, et al.;   J. Ethnopharmacol. 173, 91 (2015), Application(s): ELISA   using mouse RAW264.7 cells , Abstract;

[24]

Lipin-1 contributes to modified low-density   lipoprotein-elicited macrophage pro-inflammatory responses: A.R. Navratil, et   al. ; Atherosclerosis 242, 424 (2015), Application(s):   ELISA using mouse cell culture supernatant, Abstract;

[25]

Molecular characterization and knock-down of salmon   louse (Lepeophtheirus salmonis) prostaglandin E synthase: C. Eichner, et al.;   Exp. Parasitol. 159, 79 (2015), Application(s): ELISA Kit   measuring PGE2 levels in salmon lice, Abstract;

[26]

Prostaglandin E2   Production and T Cell Function in Mouse Adenovirus Type 1 Infection following   Allogeneic Bone Marrow Transplantation: M.K. McCarthy, et al.; PLoS One 10, e0139235 (2015), Application(s): PGE2 concentration   in bronchoalveolar lavage fluid , Abstract; Full Text

[27]

Role of gastric   acid inhibition, prostaglandins and endogenous-free thiol groups on the   gastroprotective effect of a proteolytic fraction from Vasconcellea   cundinamarcensis latex: A. Silva, et al.; J. Pharm. Pharmacol. 67, 133 (2015), Application(s): EIA using rat tissue   samples, Abstract; Full Text

[28]

Supplement of   5-hydroxytryptophan before induction suppresses inflammation and   collagen-induced arthritis: T.H. Yang, et al. ; Arthritis. Res. Ther. 17, 364 (2015), Application(s): Measurement of PGE2   in mice serum samples, Abstract; Full Text

[29]

The cancer pain related factors affected by celecoxib   together with cetuximab in head and neck squamous cell carcinoma: Y. Yang, et   al.; Biomed. Pharmacother. 70, 181 (2015),   Application(s): ELISA using human culture medium, Abstract;

[30]

The Receptor   CMRF35-Like Molecule-1 (CLM-1) Enhances the Production of LPS-Induced   Pro-Inflammatory Mediators during Microglial Activation: A. Ejarque-Ortiz, et   al.; PLoS One 10, e0123928   (2015), Application(s):   ELISA using mouse microglial supernatant, Abstract; Full Text

[31]

Inhibition of   COX-2 and PGE2 in LPS-stimulated RAW264.7 cells by lonimacranthoide VI, a   chlorogenic acid ester saponin: F. Guan, et al.; Biomed. Rep. 2, 760 (2014), Application(s): Assay of   LPS-stimulated RAW264.7 macrophages, Abstract; Full Text

[32]

Possible roles of   COX-1 in learning and memory impairment induced by traumatic brain injury in   mice: J. Shang, et al.; Braz. J. Med. Biol. Res. 47, 1050 (2014), Application(s): Analysis of PGE2 levels in mouse hippocampus tissue, Abstract; Full   Text

[33]

Prostaglandin E2   promotes features of replicative senescence in chronically activated human   CD8+ T cells: J.P. Chou, et al.; PLoS One 9, e99432 (2014), Application(s): EIA using cell culture supernatants, Abstract; Full Text

[34]

Anti-inflammatory, antioxidative and matrix   metalloproteinase inhibitory properties of 20(R)-ginsenoside Rh2 in cultured   macrophages and keratinocytes: W.Y. Choi, et al.; J. Pharm. Pharmacol. 65, 310 (2013), Application(s): EIA using culture supernatants,   Abstract;

[35]

Colorectal carcinoma-derived fibroblasts modulate   natural killer cell phenotype and antitumor cytotoxicity: T. Li, et al.; Med.   Oncol. 30, 663 (2013), Application(s): EIA using culture   supernatants, Abstract;

[36]

Melanoma-educated CD14+ cells acquire a myeloid-derived   suppressor cell phenotype through COX-2-dependent mechanisms: Y. Mao, et al.;   Cancer Res. 73, 3877 (2013), Application(s): EIA using   culture supernatants, Abstract;

[37]

Microenvironment   generated during EGFR targeted killing of pancreatic tumor cells by ATC   inhibits myeloid-derived suppressor cells through COX2 and PGE2 dependent   pathway: A. Thakur, et al.; J. Transl. Med. 11, 35 (2013), Application(s): EIA using culture supernatants from 3D co-cultures, Abstract; Full   Text

[38]

Prostaglandin E2 alteration in contraceptive consumers:   as a risk factor for inflammatory diseases: M.S. Pour, et al.; Arch. Gynecol.   Obstet. 287, 1031 (2013), Application(s): EIA using human   serum, Abstract;

[39]

Transmigration of   Neural Stem Cells across the Blood Brain Barrier Induced by Glioma Cells: M.   Díaz-Coránguez, et al.; PLoS One 8, e60655 (2013), Application(s): Quantification was done in 4 day CM derived from rat   astrocytes and glioma C6 cells using ELISA, Abstract; Full Text

[40]

XH-14, a novel   danshen methoxybenzo[b]furan derivative, exhibits anti-inflammatory   properties in lipopolysaccharide-treated RAW 264.7 cells: G.M. Park, et al.;   J. Inflamm. 10, 1 (2013), Application(s): EIA using culture   supernatants, Abstract;   Full Text

[41]

Acacetin protects   dopaminergic cells against 1-methyl-4-phenyl-1, 2, 3,   6-tetrahydropyridine-induced neuroinflammation in vitro and in vivo: H.G.   Kim, et al.; Biol. Pharm. Bull. 35, 1287 (2012), Application(s): EIA using culture supernatants, Abstract; Full Text

[42]

Hepatocellular carcinoma-associated fibroblasts trigger   NK cell dysfunction via PGE2 and IDO: T. Li, et al.; Cancer Lett. 318, 154 (2012), Application(s): EIA using culture   supernatants, Abstract;

[43]

Lycorine inhibits lipopolysaccharide-induced iNOS and   COX-2 up-regulation in RAW264.7 cells through suppressing P38 and STATs   activation and increases the survival rate of mice after LPS challenge: J.   Kang, et al.; Int. Immunopharmacol. 12, 249 (2012),   Application(s): EIA using culture supernatants, Abstract;

[44]

Matrix metalloproteinase-19 is a key regulator of lung   fibrosis in mice and humans: G. Yu, et al.; Am. J. Respir. Crit. Care Med. 186, 752 (2012), Application(s): PGE2 from mice BAL was   measured, Abstract;

[45]

Delta6-desaturase (FADS2) deficiency unveils the role   of omega3- and omega6-polyunsaturated fatty acids: W. Stoffel, et al.; EMBO   J. 27, 2281 (2008), Application(s): EIA using tissue,   Abstract;

[46]

Anti-inflammatory effects of isoflavone powder produced   from soybean cake: T.H. Kao, et al.; J. Agric. Food Chem. 55,   11068 (2007), Application(s): EIA using peritoneal exudate fluid and   peritoneal exudate cell supernatant, Abstract;

[47]

Celecoxib inhibits Cdx2 expression and prevents gastric   cancer in Helicobacter pylori-infected Mongolian gerbils: S. Futagami, et   al.; Digestion 74, 187 (2006), Abstract;

[48]

Spinal   inflammatory hyperalgesia is mediated by prostaglandin E receptors of the EP2   subtype: H. Reinold, et al.; J. Clin. Invest. 115, 673 (2005), Application(s): PGE2 from the thoracolumbar segment of the spinal cord was   measured, mice, Abstract;   Full Text

[49]

Effect of a selective cyclooxygenase-2 inhibitor,   nimesulide, on the growth of lung tumors and their expression of   cyclooxygenase-2 and peroxisome proliferator- activated receptor-gamma: M.S.   Shaik, et al.; Clin. Cancer Res. 10, 1521 (2004),   Application(s): EIA using mouse tissue, Abstract;

[50]

Nonopioid actions of intrathecal dynorphin evoke spinal   excitatory amino acid and prostaglandin E2 release mediated by   cyclooxygenase-1 and -2: L. Koetzner, et al.; J. Neurosci. 24,   1451 (2004), Application(s): EIA using dialysate, Abstract;

[51]

Differential expression of platelet-activating factor   acetylhydrolase in macrophages and monocyte-derived dendritic cells: S.   Al-Darmaki, et al.; J. Immunol. 170, 167 (2003),   Application(s): EIA using human culture supernatants, Abstract;

[52]

Inhibition of cyclooxygenase-2 by rofecoxib attenuates   the growth and metastatic potential of colorectal carcinoma in mice: M. Yao,   et al.; Cancer Res. 63, 586 (2003), Application(s):   Concentration of PGE2 in splenic tumor cystosolic fractions were determined   using mouse tissue, Abstract;

[53]

Microsomal   prostaglandin E synthase-1 is a major terminal synthase that is selectively   up-regulated during cyclooxygenase-2-dependent prostaglandin E2 production in   the rat adjuvant-induced arthritis model: D. Claveau, et al.; J. Immunol. 170, 4738 (2003), Application(s): PGE2 production was   determined by enzyme immunoassay analysis using rat tissue, Abstract; Full   Text

[54]

Increased levels of the pro-inflammatory prostaglandin   PGE2 in CSF from ALS patients: G. Almer, et al.; Neurology 58,   1277 (2002), Application(s): EIA using cerebral spinal fluid, Abstract;

[55]

The effects of periodontal therapy on intracrevicular   prostaglandin E2 concentrations and clinical parameters in pregnancy: F.   Yalcin, et al.; J. Periodontol. 73, 173 (2002),   Application(s): EIA using gingival crevicular fluid, Abstract;

[56]

Promoter   methylation regulates Helicobacter pylori-stimulated cyclooxygenase-2   expression in gastric epithelial cells: M. Akhtar, et al.; Cancer Res. 61, 2399 (2001), Application(s): EIA   using cell culture supernatant, Abstract; Full Text

[57]

Inflammatory agonists induce cyclooxygenase type 2   expression by human neutrophils: C.G. Maloney, et al.; J. Immunol. 160, 1402 (1998), Application(s): EIA using human cell culture   supernatant, Abstract;

[58]

Selective cyclooxygenase-2 inhibition by nimesulide in   man: L. Cullen, et al.; J. Pharmacol. Exp. Ther. 287, 578   (1998), Application(s): EIA using plasma, Abstract;

产品编号 产品名称 产品规格 产品等级
ADI-900-001 PGE2 ELISA kit 
前列腺素E2酶联免疫试剂盒		 96 wells –
ADI-901-001 PGE2 ELISA kit 
前列腺素E2酶联免疫吸附试剂盒		 5×96 wells –
ENZ-KIT179-0200 PLAQPRO™ Lp-PLA2 Assay 
PLAQPRO™ Lp-PLA2 化验		 200 tests –
ADI-930-001 PGE2 high sensitivity ELISA kit
PGE2高灵敏度酶联免疫试剂盒		 96 wells –
ADI-931-001 PGE2 high sensitivity ELISA kit 
前列腺素E2 高灵敏度 ELISA kit		 5×96 wells –
ADI-900-068 LTB4 ELISA kit 
白三烯B4 酶联免疫试剂盒		 96 wells –
ADI-901-068 LTB4 ELISA kit 
白三烯B4 酶联免疫试剂盒		 5×96 wells –
ADI-900-092 11-dehydro-TXB2 ELISA kit 
11-脱氢-血栓烷素B2 ELISA试剂盒		 96 wells –

MCI Buffer L-8500-PH-RG MCI缓冲溶液 L-8500-PH-RG 品牌：Mitsubishi Chem

发表于由

品牌：Mitsubishi Chem
CAS No.：
储存条件：2-10℃
纯度：–
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

638-12511

 – 1 L×2 – 1,410.00 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

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cAMP & cGMP 检测试剂盒 cAMP & cGMP ELISA Kit

发表于由
  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

cAMP & cGMP 检测试剂盒cAMP & cGMP 检测试剂盒 cAMP & cGMP ELISA Kit

 cAMP & cGMP ELISA Kit



◆原理

 

　　环状核苷酸可作为G蛋白信号通道和钙离子信号通道的第二信使。

　　环状AMP（cAMP）是重要的第二信使，在很多生物学效应中起作用。已知大量激素通过能将ATP转变成cAMP的腺苷酸环化酶去激活cAMP。cAMP与血管疾病，神经系统，免疫，细胞生长和分化，代谢等有关。

　　环状GMP一般比cAMP的含量水平低10~100倍，鸟苷酸环化酶作用在GTP上形成cGMP。一些激素，比如乙酰胆碱，胰岛素，催产素和其他特定的化合物比如血清素，组胺会引起cGMP水平的升高。刺激鸟苷酸环化酶的化合物，比如血管舒张药硝普钠，硝化甘油，硝酸钠和一氧化氮会提高cGMP的含量。

 

　　相关疾病：心脏疾病，肿瘤，疼痛，哮喘，肥胖症，药物成瘾，精神疾病。

 


◆特点


●   兼容性好：可检测细胞裂解液，细胞培养上清，血清，血浆，唾液，组织或者尿液样品。可检测任意物种。

●   灵敏度高：优化的乙酰化步骤可以提高样品的检测灵敏度（可提高10倍）。

●   稳定性好：批次间的产品质量表现一致。

●   稳定性好：可用于高通量筛选，3小时完成检测。

●   稳定性好：简单，高效的样品处理步骤。

 


◆案例


●   检测小鼠视网膜裂解液

●   检测斑马鱼幼虫

●   检测软骨细胞匀浆液

●   检测N. benthamiana叶子抽提物

●   检测HUVEC细胞裂解液

●   检测大鼠肝脏组织

●   检测大鼠脑部匀浆液

cAMP  

 

Species    Reactivity

Sample Type

Sensitivity

Range

Prod. No.

Size

　cAMP CompleteEIA kit　

Species independent　

Cell lysates, culture su-pernatants,    serum, saliva and tissue　

Assay Buffer: 0.30 pmol/ml(non-acetylated) 0.039 pmol/mL (acetylated)HCl: 0.39 pmol/mL (non-acetylated)0.037 pmol/mL (acetylated)

0.78 – 200 pmol/mL (non-acetylated);    0.078 – 20 pmol/mL (acetylated)　

ADI-900-163

 

96 wells

　

ADI-900-163　

 5×96  wells

　Direct cAMP EIA kit

Species independent

Cell lysates and tissue

(non-acetylated) 0.39 pmol/mL;
 (acetylated) 0.037 pmol/mL

 0.78 – 200 pmol/mL (non- acetylated); 0.078 – 20 pmol/mL  (acetylated)

ADI-900-066

 

96 wells

ADI-901-066

5×96 wells

　cAMP EIA kit

Species independent

 Culture supernatants, serum and saliva

 (non-acetylated) 0.30 pmol/mL;
(acetylated) 0.039 pmol/mL

 0.78 –- 200 pmol/mL (non-acetylated); 0.078 –20 pmol/mL (acetylated)

ADI-900-067

96 wells

ADI-901-067

5×96 wells

 


cGMP


Species    Reactivity

Sample Type

Sensitivity

Range

Prod. No.

Size

　cGMP CompleteEIA kit

Species independent　

Cell lysates, culturesupernatants, plasma,serum, saliva, tissue andurine　

Assay Buffer : 0.42 pmol/ml(non-acetylated) 0.043 pmol/mL(acetylated) HCl: 0.604 pmol/mL (non-acetylated) 0.059 pmol/mL (acetylated)

0.8 – 500 pmol/mL (non-acetylated);  0.08 – 50pmol/mL   (acetylated)　

ADI-900-164

 

96  wells

　

ADI-901-164

 5×96  wells

　Direct cGMP EIA kit

Species independent　

Cell lysates and tissue　

(non-acetylated) 0.604 pmol/mL;(acetylated) 0.025 pmol/mL

0.8-500 pmol/mL (non-acetylated);  0.08 -50 pmol/mL   (acetylated)

ADI-900-014


96  wells

 


ADI-901-014

 5×96  wells　

　cGMP EIA kit

Species independent　

 Culture supernatants,plasma, serum, salivaand urine

 (non-acetylated) 0.37 pmol/mL;(acetylated) 0.088 pmol/mL　

0.16-500 pmol/mL (non-acetylated);   0.16-100 pmol/mL  (acetylated)

ADI-900-013


96  wells

 


ADI-901-013

 5×96  wells　



Q1：　乙酰化步骤是否是必须的？

A1：　如果样品中含有的cAMP或者cGMP浓度低或者想要提高检测灵敏度的话，样品需要进行乙酰化。乙酰化的

　　   灵敏度大概是非乙酰化的10倍多 。如果样品中的环状核苷酸的水平未知，推荐先检测几个样品（样品不进

　　   行乙酰化处理）。如果检测不到的话，样品进行乙酰化处理。

 

Q2：　是否可以用Complete ELISA试剂盒检测胞质中的cAMP/cGMP？

A2：　这些是比色法的ELISA试剂盒，定量检测任意物种的细胞裂解液，细胞培养上清，血清、唾液和组织样品

　　   （细胞内和细胞外的样品）的AMP/cGMP。

 

Q3：　我是否可用自己的裂解buffer（lysis buffer）?

A3：　当使用偶联物（conjunction）和中和试剂时，该试剂盒已经使用HCl裂解样品和稀释标准品。如果使用不

　　   同的buffer，并且用HCl稀释的话，反应的pH值不同（比如如果使用其他试剂，那么设计为了中和HCl的

　　   中和试剂不具有校对作用）。因此，为了优化结果，建议使用HCl进行细胞裂解和稀释。

 

Q4：　我样品中的cAMP/cGMP希望的浓度是多少？

A4：　样品中的cAMP/cGMP的量取决于样品的类型，来源甚至处理的方法。不幸的话，这个不是我们能够帮助

　　   您帮您预测的。建议使用者查找相关资料，在这些资料红，研究的是类似的样品，条件或者检测技术。

 

Q5：　用HCl处理的裂解液与哪些蛋白检测兼容？

A5：　最好先研究一下您希望使用的蛋白检测产品。比如，Pierc有不同的BCA检测试剂盒，在他们网站都有列出

　　   每个试剂盒兼容的试剂。我们建议直接和蛋白检测试剂的制造商或者销售商联系。他们应该可以提供相关

　　   解答。

 

Q6：　如果我的细胞在加入0.1M HCl后仍然没有裂解，该如何处理？

A6：　在使用0.1M HCl前，加入 Triton X-100（曲拉通 X-100）能够增加细胞或者组织的裂解。在这个浓度范围

　　   内使用，阴离子表面活性剂不会干扰乙酰化或者检测中结合的部分。然而，在最终检测OD值时，OD值会

　　   有一些增加。所以，含有Triton X-100的样品检测时，相应的标准品种也要添加Triton X-100。

 

Q7：　我需要使用多少样品？建议的稀释度是多少？

A7：　样品的用量和稀释度取决于样品中含有的cAMP/cGMP。建议客户可以参考相关的文章，该文章使用的是

　　   类似的样品类型，条件和检测技术。

 

Q8：　需要使用多少细胞？

A8：　组织的重量和细胞的数目完全取决于实验的条件。建议先测一两个样品，细胞的数目大概在2~5×106

　　   细胞/mL HCl。根据实验结果，有些样品可能需要进一步稀释。记住，稀释母液的方法比重新开始实验要容

　　   易些。

 

Q9：　是否可以用Direct ELISA试剂盒检测胞质的cAMP/cGMP？

A9：　该系列试剂盒可用于检测任意物种细胞或者组织裂解液的cAMP/cGMP。

 

Q10：　在用cAMP/cGMP ELISA或者cAMP/cGMP Complete ELISA试剂盒检测细胞培养上清的cAMP/cGMP

　　   时是否可以使用细胞培养基？

A10：　按照说明书所说，只要用含有分泌cAMP/cGMP的同样培养基稀释标准品。OD值上发生的细微变化与样品

　　   和标准品放置的板具有可比性。在研发这些试剂盒时，使用了cAMP/cGMP加样的含有酚红的PRMI1640

　　   和DMEM+Glutamax，OD值略有增加，样品也同样。建议优化自己的样品稀释度，对自己样品进行加样/

　　   回收测试，这样会得到更好的回收率和线性数据。

 

Q11：　乙酰化标准品和样品的最大结合率只有50%，为什么？

A11：　如果样品和标准品都已经乙酰化了，但是Bo标准品没有乙酰化的话，会出现上述情况。您的数据仍然是可

　　   用的。计算样品浓度的OD值，不计算%B/B0分析。

 

Q12：　哪些ELISA试剂盒能够检测胞质cAMP/cGMP？

A12：　Direct ELISA 试剂盒可以检测细胞内的cAMP/cGMP。如果想要检测胞内和细胞外的样品，可以使用

　　   complete ELISA试剂盒。

 

Q13：　是否可用这些试剂盒去研究激动剂和拮抗剂？

A13：　如果样品中的cAMP/cGMP符合试盒的检测范围，那么这些试剂盒可用于检测激动剂和拮抗剂引起的变化。

　　   当然，使用者最好建立样品中cAMP/cGMP 水平的基本线，优化激动剂和拮抗剂的处理。

 

Q14：　用assay buffer重悬后样品可以保存多久？

A14：　样品重悬后的稳定性不能评估。建议重悬后尽快使用。建议将样品保存在-80℃。

 

Q15：　干燥样品的话，如何处理？

A15：　使用真空离心（比如SpeedVac）或者氮气或者氩气进行干燥样品。如果样品多，建议使用真空离心的方

　　   法。样品可以在SpeedVac 室温放置过夜。第二天干燥，但是不能保证产品的完整性（因为不是在真空环

　　   境中保存样品）。

　　   用氮气干燥样品需要在气体末端使用玻璃枪头，将枪头正好悬在含有你样品的管子内。如果使用这个方

　　   法，建议首先在含有石油醚的管子上操作，确定操作流程的时间。

 

Q16：　用cAMP ELISA试剂盒Assay buffer稀释的样品，是否可用cGMP ELISA 试剂盒检测？

A16：　这两个试剂盒的Assay Buffer的货号是一样的，所以产品都是一样的。如果使用试剂盒其他所有组分，样

　　   品也没有问题。

 

Q17：　我是否可以保存乙酰化样品一段时间，晚点再测？

A17：　不。我们建议马上检测的样品才进行乙酰化处理。样品不添加试剂进行乙酰化处理时更稳定。

 

Q18：　我细胞的样品检测后数值不是很一致。我认为我的细胞可能穿孔了。

A18：　为了获得更一致的结果，我们建议检测时用裂解液彻底破坏细胞。由于细胞的厚度不一，建议用显微镜检

　　   查一下。

 

Q19：　需要使用多少assay buffer去重悬我的血清样品？

A19：　重悬样品的assay buffer体积完全取决于具体的实验情况。我们建议1mL assay buffer重悬1mL血清，然

　　   后进行一定的稀释。

 

Q20：　考虑到板子是用抗兔的IgG包被的，是否可用来检测兔血清的cAMP/cGMP？

A20：　该试剂盒提供的板子包被的是能够捕获cAMP/cGMP的通用抗体。试剂盒使用的山羊抗兔IgG的抗体，意味

　　   着含有高内源性IgG的兔样品会引起干扰，影响cAMP/cGMP的检测。如果必须检测这些样品，我们推荐在

　　   检测之前使用MWCO过滤或IgG去除试剂盒。请注意，我们没有测试过上述方法，也不能提供相关实验流程。

 

◆cAMP

 cAMP complete ELISA kit

 

Product Literature References

Arginine Thiazolidine Carboxylate Stimulates Insulin Secretion through Production of Ca2+-Mobilizing Second Messengers NAADP and cADPR in Pancreatic Islets: D.R. Park, et al.; PLoS One 10, e0134962 (2015), Application(s):ELISA using acetylated mouse islets, Abstract; Full Text

Cytotoxicity and Biological Efficacy of Exendin-4-Encapsulated Solid Lipid nanoparticles in INS-1 Cells: H. Jun, et al.; J. Nanomater. 2015, Article ID 753569 (2015), Application(s): ELISA using rat INS-1 cells, Full Text

Hepatic ANGPTL3 regulates adipose tissue energy homostasis: Y. Wang, et al.; PNAS 112, 11630 (2015), Application(s):ELISA using mouse tissue homogenate, Abstract; Full Text

Misoprostol modulates cytokine expression through a cAMP pathway: Potential therapeutic implication for liver disease: L. Gobejishvili, et al.; Cell Immunol. 6616, 30041 (2015), Application(s): ELISA kits quantifying cell lysates, Abstract;

Natural products induce a G protein-mediated calcium pathway activating p53 in cancer cells: P.R. van Ginkel, et al.; Toxicol. Appl. Pharmacol. (2015), Application(s): ELISA using MDA-MB-231 cells, Abstract;

Sinensetin Enhances Adipogenesis and Lipolysis by Increasing Cyclic Adenosine Monophosphate Levels in 3T3-L1 Adipocytes: S.I. Kang, et al.; Biol. Pharm. Bull. 38, 552 (2015), Application(s): ELISA, Abstract; Full Text

cGMP inhibition of type 3 phosphodiesterase is the major mechanism by which C-type natriuretic peptide activates CFTR in the shark rectal gland: H.R. De Jonge, et al.; Am. J. Physiol. Cell Physiol. 306, C343 (2014), Application(s): ELISA using dogfish shark supernatant, Abstract; Full Text

A role for GPRx, a novel GPR3/6/12-related G-protein coupled receptor, in the maintenance of meiotic arrest in Xenopus laevis oocytes: D. Ríos-Cardona, et al.; Dev. Biol. 317, 380 (2008), Application(s): EIA using xenopus cell lysates,Abstract;

General Literature References

Central role for cAMP signaling in pigmentation and UV resistance: J. D'Orazio & D.E. Fisher; Cell Cycle 10, 8 (2011),Abstract;

 

cAMP complete ELISA kit

 

Product Literature References

Arginine Thiazolidine Carboxylate Stimulates Insulin Secretion through Production of Ca2+-Mobilizing Second Messengers NAADP and cADPR in Pancreatic Islets: D.R. Park, et al.; PLoS One 10, e0134962 (2015), Application(s):ELISA using acetylated mouse islets, Abstract; Full Text

Cytotoxicity and Biological Efficacy of Exendin-4-Encapsulated Solid Lipid nanoparticles in INS-1 Cells: H. Jun, et al.; J. Nanomater. 2015, Article ID 753569 (2015), Application(s): ELISA using rat INS-1 cells, Full Text

Hepatic ANGPTL3 regulates adipose tissue energy homostasis: Y. Wang, et al.; PNAS 112, 11630 (2015), Application(s):ELISA using mouse tissue homogenate, Abstract; Full Text

Misoprostol modulates cytokine expression through a cAMP pathway: Potential therapeutic implication for liver disease: L. Gobejishvili, et al.; Cell Immunol. 6616, 30041 (2015), Application(s): ELISA kits quantifying cell lysates, Abstract;

Natural products induce a G protein-mediated calcium pathway activating p53 in cancer cells: P.R. van Ginkel, et al.; Toxicol. Appl. Pharmacol. (2015), Application(s): ELISA using MDA-MB-231 cells, Abstract;

Sinensetin Enhances Adipogenesis and Lipolysis by Increasing Cyclic Adenosine Monophosphate Levels in 3T3-L1 Adipocytes: S.I. Kang, et al.; Biol. Pharm. Bull. 38, 552 (2015), Application(s): ELISA, Abstract; Full Text

cGMP inhibition of type 3 phosphodiesterase is the major mechanism by which C-type natriuretic peptide activates CFTR in the shark rectal gland: H.R. De Jonge, et al.; Am. J. Physiol. Cell Physiol. 306, C343 (2014), Application(s): ELISA using dogfish shark supernatant, Abstract; Full Text

A role for GPRx, a novel GPR3/6/12-related G-protein coupled receptor, in the maintenance of meiotic arrest in Xenopus laevis oocytes: D. Ríos-Cardona, et al.; Dev. Biol. 317, 380 (2008), Application(s): EIA using xenopus cell lysates,Abstract;

General Literature References

Central role for cAMP signaling in pigmentation and UV resistance: J. D'Orazio & D.E. Fisher; Cell Cycle 10, 8 (2011),Abstract;

 

Direct cAMP ELISA kit

 

Product Literature References

Impaired enzymatic defensive activity, mitochondrial dysfunction and proteasome activation are involved in RTT cells oxidative damage: C. Cervellati, et al.; Biochim. Biophys. Acta. (2015), Application(s): ELISA using human supernatant,Abstract;

MEK Inhibitors Reverse cAMP-Mediated Anxiety in Zebrafish: P.R. Lundegaard, et al.; Chem. Biol. 15, 330 (2015),Application(s): ELISA determining cAMP concentration in zebrafish larvae, Abstract;

Temporal cAMP signaling selectivity by natural and synthetic MC4R agonists: B.M. Molden, et al.; Mol. Endocrinol. (2015),Application(s): cAMP determination by immunoassay, Abstract; Full Text

The cAMP phosphodiesterase Prune localizes to the mitochondrial matrix and promotes mtDNA replication by stabilizing TFAM: F. Zhang, et al.; EMBO Rep. (2015), Application(s): ELISA, Abstract; Full Text

The neuroprotective effect of lithium in cannabinoid dependence is mediated through modulation of cyclic AMP, ERK1/2 and GSK-3β phosphorylation in cerebellar granular neurons of rat: H.R. Rahimi, et al.; Iran. J. Pharm. Res. 14, (2015),Application(s): ELISA using rat supernatant, Full Text

Ubiquitin is a versatile scaffold protein for the generation of molecules with de novo binding and advantageous drug-like properties: F. Job, et al.; FEBS Open Bio 5, 579 (2015), Application(s): ELISA using cell lysates, Abstract; Full Text

Adenosine 2A receptor promotes collagen production by human fibroblasts via pathways involving cyclic AMP and AKT but independent of Smad2/3: M. Perez-Aso, et al.; FASEB J. 28, 802 (2014), Abstract;

Cyclin-dependent kinase 5 in the ventral tegmental area regulates depression-related behaviors: P. Zhong, et al.; J. Neurosci. 34, 6352 (2014), Application(s): EIA using mouse ventral tegmental area (VTA) homogenates, Abstract; Full Text

DCC is expressed in a CD166-positive subpopulation of chondrocytes in human osteoarthritic cartilage and modulates CRE activity: A.K. Bosserhoff, et al.; Int. J. Clin. Exp. Pathol. 7, 1947 (2014), Application(s): EIA using chondrocyte homogenates, Abstract; Full Text

Disruption of NHE8 Expression Impairs Leydig cell function in the testes: H. Xu, et al.; Am. J. Physiol. Cell Physiol. (2014),Abstract;

Dopamine-2 receptor activation suppresses PACAP expression in gonadotrophs: S.J. Winters, et al.; Endocrinology 155, 2647 (2014), Abstract;

Global analysis of the HrpL regulon in the plant pathogen Pseudomonas syringae pv. tomato DC3000 reveals new regulon members with diverse functions: H.N. Lam, et al.; PLoS One 9, e106115 (2014), Application(s): EIA using leaf extracts from N. benthamiana, Abstract; Full Text

High throughput synthetic lethality screen reveals a tumorigenic role of adenylate cyclase in fumarate hydratase-deficient cancer cells: M. Boettcher, et al.; BMC Genomics 15, 158 (2014), Application(s): EIA using human hereditary leiomyomatosis and renal cell carcinoma (HLRCC) lysates, Abstract; Full Text

Identification of plumericin as a potent new inhibitor of the NF-κB pathway with anti-inflammatory activity in vitro and in vivo: N. Fakhrudin, et al.; Br. J. Pharmacol. 171, 1676 (2014), Application(s): EIA using HUVECtert lysates (acetylated samples), Abstract; Full Text

Lipid droplet protein LID-1 mediates ATGL-1-dependent lipolysis during fasting in Caenorhabditis elegans: J.H. Lee, et al.; Mol. Cell. Biol. 34, 4165 (2014), Abstract;

Low concentrations of metformin suppress glucose production in hepatocytes through AMP-activated protein kinase (AMPK): J. Cao, et al.; J. Biol. Chem. 289, 20435 (2014), Abstract;

Prostaglandin E2 promotes features of replicative senescence in chronically activated human CD8+ T cells: J.P. Chou, et al.; PLoS One 9, e99432 (2014), Application(s): EIA using cell culture supernatants, Abstract; Full Text

Regulator of G-protein signaling 6 (RGS6) promotes anxiety and depression by attenuating serotonin-mediated activation of the 5-HT(1A) receptor-adenylyl cyclase axis: A. Stewart, et al.; FASEB J. 28, 1735 (2014), Abstract;

Resveratrol and curcumin enhance pancreatic β-cell function by inhibiting phosphodiesterase activity: M. Rouse, et al.; J. Endocrinol. 223, 107 (2014), Application(s): EIA using mouse β-Min6 lysates and primary human islet lysates, Abstract;Full Text

The metabolite 5'-methylthioadenosine signals through the adenosine receptor A2B in melanoma: K. Limm, et al.; Eur J. Cancer 50, 2714 (2014), Abstract;

Three intragenic suppressors of a GTPase-deficient allele of GNAS associated with McCune-Albright syndrome: K. Turcic, et al.; J. Mol. Endocrinol. 52, 321 (2014), Abstract;

Age-dependent alterations in cAMP signaling contribute to synaptic plasticity deficits following traumatic brain injury: D.J. Titus, et al.; Neuroscience 231, 182 (2013), Application(s): EIA using rat cortex, hippocampus and thalamus homogenates, Abstract; Full Text

Calcium supplementation increases circulating cholesterol by reducing its catabolism via GPER and TRPC1-dependent pathway in estrogen deficient women: S. Li, et al.; Int. J. Cardiol. 168, 2548 (2013), Application(s): ELISA using rat liver tissue, Abstract;

Does 4-tert-octylphenol affect estrogen signaling pathways in bank vole Leydig cells and tumor mouse Leydig cells in vitro?: M. Kotula-Balak, et al.; Reprod. Toxicol. 39, 6 (2013), Abstract;

Liraglutide protects against amyloid-β protein-induced impairment of spatial learning and memory in rats: W.N. Han, et al.; Neurobiol Aging 34, 576 (2013), Application(s): EIA using rat brain homogenates, Abstract;

Molecular mechanisms underlying the rapid arrhythmogenic action of bisphenol A in female rat hearts: X. Gao, et al.; Endocrinology 154, 4607 (2013), Application(s): EIA using rat myocyte homogenates, Abstract; Full Text

Resveratrol prevents β-cell dedifferentiation in nonhuman primates given a high-fat/high-sugar diet: J.L. Fiori, et al.; Diabetes 62, 3500 (2013), Application(s): EIA using CHO cell supernatant, Abstract; Full Text

Role of AKAP79/150 protein in b1-adrenergic receptor trafficking and signaling in mammalian cells: X. Li, et al.; J. Biol. Chem. 288, 33797 (2013), Application(s): ELISA using murine cardiomyocytes, Abstract;

Rolofylline, an adenosine A1 receptor antagonist, inhibits osteoclast differentiation as an inverse agonist: W. He, et al.; Br. J. Pharmacol. 170, 1167 (2013), Application(s): EIA using bone marrow macrophage lysates (acetylated samples),Abstract; Full Text

Constitutive cholesterol-dependent endocytosis of melanocortin-4 receptor (MC4R) Is essential to maintain receptor responsiveness to α-melanocyte-stimulating hormone (α-MSH): F.K. McDaniel, et al.; J. Biol. Chem. 287, 21873 (2012),Application(s): ELISA using human neuronal cells, Abstract;

Drugs developed to treat diabetes, liraglutide and lixisenatide, cross the blood brain barrier and enhance neurogenesis: K. Junter & C. Hölscher ; BMC Neurosci. 13, 33 (2012), Application(s): ELISA analysis of mouse brain tissue, Abstract;Full Text

Liraglutide, a long-acting GLP-1 mimetic, and its metabolite attenuate inflammation after intracerebral hemorrhage: J. Hou, et al.; J. Cereb. Blood Flow Metab. 32, 2201 (2012), Application(s): ELISA analysis of mouse brain tissue, Abstract;Full Text

Pharmacological validation of Trypanosoma brucei phosphodiesterases as novel drug targets: H.P. de Koning, et al.; J. Infect. Dis. 206, 229 (2012), Application(s): EIA using Trypanosoma lysates, Abstract; Full Text

Regulation of pancreatic cancer by neuropsychological stress responses: a novel target for intervention: H.M. Schuller, et al.; Carcinogenesis 33, 191 (2012), Application(s): ELISA using human pancreatic cancer xenografts, Abstract;

Acylated and unacylated ghrelin attenuate isoproterenol-induced lipolysis in isolated rat visceral adipocytes through activation of phosphoinositide 3-kinase γ and phosphodiesterase 3B: A. Baragli, et al.; Biochim. Biophys. Acta 1811, 386 (2011), Application(s): EIA using rat adipocyte extracts, Abstract;

Characterization of the differential response of endothelial cells exposed to normal and elevated laminar shear stress: S.J. White, et al.; J. Cell. Physiol. 226, 2841 (2011), Application(s): ELISA using HUVEC, Abstract; Full Text

cAMP pulsing of denuded mouse oocytes increases meiotic resumption via activation of AMP-activated protein kinase: Chen J., et al.; Reproduction 138, 759 (2009), Abstract;

Heat-Labile Enterotoxin Promotes Escherichia coli Adherence to Intestinal Epithelial Cells: P. Hardwidge, et al. ; J. Bacteriol. 191, 178 (2009), Application(s): EIA using porcine cell lysates, Abstract;

PPARγ agonists inhibit vasopressin-mediated anion transport in the MDCK-C7 cell line: C. Nofziger, et al.; Am. J. Physiol. Renal Physiol. 297, F55 (2009), Application(s): ELISA using MDCK-C7 cell lysates, Abstract; Full Text

Androgens transduce the G-alpha s-mediated activation of protein kinase A in prostate cells: Y. Daaka, et al. ; Cancer Res. 68, 3225 (2008), Application(s): EIA using human cell lysates, Abstract;

beta-Arrestin-1 mediates glucagon-like peptide-1 signaling to insulin secretion in cultured pancreatic beta cells: J. Olefsky, et al. ; PNAS 105, 6614 (2008), Application(s): EIA using rat cell lysates, Abstract;

Cyclic Adenosine Monophosphate Regulation of Ion Transport in Porcine Vocal Fold Mucosae: M. Sivasankar, et al.; Laryngoscope 118, 1511 (2008), Abstract;

FMRP acts as a key messenger for dopamine modulation in the forebrain: M. Zhao, et al. ; Neuron 59, 634 (2008),Application(s): EIA using mouse cell lysates, Abstract;

Functional evidence for presence of lipid rafts in erythrocyte membranes: Gs-alpha in rafts is essential for signal transduction: Y. Takakuwa, et al. ; Am. J. Hematol. 83, 371 (2008), Application(s): EIA using human cell lysates, Abstract;

Regulation of G proteins by human 5-HT1a receptor TM3/i2 and TM5/i3 loop peptides: H.V. Thiagaraj, et al.; Neurochem. Int. 50, 109 (2007), Application(s): EIA using human cell lysates, Abstract;

Co-occurrence of Two Partially Inactivating Polymorphisms of MC3R Is Associated With Pediatric-Onset Obesity: N. Feng, et al. ; Diabetes 54, 2663 (2005), Application(s): EIA using human cell lysates, Abstract;

The hrpK Operon of Pseudomonas syringae pv. tomato DC3000 Encodes Two Proteins Secreted by the Type III (Hrp) Protein Secretion System: HopB1 and HrpK, a Putative Type III Translocator: T. Petnicki-Ocwieja, et al. ; J. Bacteriol. 187, 649 (2005), Application(s): EIA using plant cell lysates, Abstract;

High-salt diet impairs vascular relaxation mechanisms in rat middle cerebral arteries: J.H. Lombard, et al. ; Am. J. Physiol. Heart Circ. Physiol. 284, H1124 (2003), Application(s): EIA using rat cell lysates, Abstract;

Lysine 270 in the Third Intracellular Domain of the Oxytocin Receptor is an Important Determinant for G{alpha}q Coupling Specificity: M. Yang, et al. ; Mol. Endocrinol. 16, 814 (2002), Application(s): EIA using monkey cell lysates, Abstract;

General Literature References

Central role for cAMP signaling in pigmentation and UV resistance: J. D'Orazio & D.E. Fisher; Cell Cycle 10, 8 (2011),Abstract;

 

 

◆cGMP

 

cGMP ELISA kit

 

Product Literature References

Quercetin Inhibits Pacemaker Potentials via Nitric Oxide/cGMP-Dependent Activation and TRPM7/ANO1 Channels in Cultured Interstitial Cells of Cajal from Mouse Small Intestine: H. Gim, et al.; Cell. Physiol. Biochem. 35, 2422 (2015),Application(s): ELISA using murine small intestine interstitial cells of Cajal (ICCs), Abstract; Full Text

The Influence of Nitric Oxide on Soluble Guanylate Cyclase Regulation by Nucleotides: the Role of Pseudosymmetric Site: N. B. Surmeli, et al.; J. Biol. Chem. (2015), Application(s): ELISA, Abstract; Full Text

 

cGMP complete ELISA kit

 

Product Literature References

cGMP/PKG Signaling Suppresses Inositol 1,4,5-Trisphosphate Receptor Phosphorylation and Promotes Endoplasmic Reticulum Stress in Photoreceptors of CNG Channel-Deficient Mice: H. Ma, et al.; J. Biol. Chem. (2015), Application(s):ELISA using mouse retinal lysate, Abstract; Full Text

Heterozygous NPR2 mutations cause disproportionate short stature, similar to Léri-Weill dyschondrosteosis: A. Hisado-Oliva, et al.; J. Clin. Endocrinol. Metab. (2015), Application(s): ELISA using primate supernatant, Abstract; Full Text

Subcellular Trafficking of Guanylyl Cyclase/Natriuretic Peptide Receptor-A with Concurrent Generation of Intracellular cGMP: I. Nabu, et al.; Biosci. Rep. (2015), Application(s): cGMP ELISA using human cells, Abstract; Full Text

cGMP inhibition of type 3 phosphodiesterase is the major mechanism by which C-type natriuretic peptide activates CFTR in the shark rectal gland: H.R. De Jonge, et al.; Am. J. Physiol. Cell Physiol. 306, C343 (2014), Application(s): ELISA using dogfish shark supernatant, Abstract; Full Text

cGMP accumulation causes photoreceptor degeneration in CNG channel deficiency: evidence of cGMP cytotoxicity independently of enhanced CNG channel function: J. Xu, et al.; J. Neurosci. 33, 14939 (2013), Application(s): ELISA using mouse retinal lysate, Abstract; Full Text

 

Direct cGMP ELISA kit

Product Literature References

Effects of Intermittent Fasting on Age-Related Changes on Na,K-ATPase Activity and Oxidative Status Induced by LPS In Rat Hippocampus: A.R. Vasconcelos, et al.; Neurobiol. Aging (2015), Application(s): ELISA using rat brain supernatant,Abstract; Full Text

PCSK6-mediated corin activation is essential for normal blood pressure: S. Chen, et al.; Nat. Med. 21, 1048 (2015),Abstract;

Dephosphorylation and inactivation of NPR2 guanylyl cyclase in granulosa cells contributes to the LH-induced decrease in cGMP that causes resumption of meiosis in rat oocytes: J. Egbert, et al.; Development 141, 3594 (2013), Application(s):EIA using rat ovarian follicles, Abstract;

Nitric oxide synthase-3 promotes embryonic development of atrioventricular valves: Y. Liu, et al.; PLoS One 8, e77611 (2013), Application(s): EIA using mouse cardiac tissue, Abstract; Full Text

Sestrin 2 and AMPK connect hyperglycemia to Nox4-dependent endothelial nitric oxide synthase uncoupling and matrix protein expression: A. Eid, et al.; Mol. Cell. Biol. 33, 3439 (2013), Application(s): EIA using rat glomerular mesangial cells,Abstract; Full Text

β-Sitosterol oxidation products attenuate vasorelaxation by increasing reactive oxygen species and cyclooxygenase-2: C. Yang, et al.; Cardiovasc. Res. 97, 520 (2013), Application(s): ELISA using rat aortic tissue, Abstract; Full Text

Chronic deficiency of nitric oxide affects hypoxia inducible factor-1α (HIF-1α) stability and migration in human endothelial cells: M.G. Cattaneo, et al.; PLoS One 6, e29680 (2011), Application(s): EIA using human cell lysate, Abstract; Full Text

cGMP secreted from the tapeworm Hymenolepis diminuta is a signal molecule to the host intestine: N. Zimmerman, et al. ; J. Parasitol. 94, 771 (2008), Application(s): EIA using tapeworm culture supernatants, Abstract;

Evidence for functional heterogeneity of circulating B-type natriuretic peptide: A. Protter, et al. ; J. Am. Coll. Cardiol. 49, 1071 (2007), Application(s): EIA using human cell lysates, Abstract;

Estradiol and progesterone modulate the nitric oxide/cyclic gmp pathway in the hypothalamus of female rats and in GT1-1 cells: H.P. Chu, et al. ; Endocrine 24, 177 (2004), Application(s): EIA using rat cell lysates, Abstract;

A Novel PDZ Protein Regulates the Activity of Guanylyl Cyclase C, the Heat-stable Enterotoxin Receptor: R.O. Scott, et al. ; J. Biol. Chem. 277, 22934 (2002), Application(s): EIA using monkey cell lysates, Abstract;

The Mechanism of Ciliary Stimulation by Acetylcholine: Roles of Calcium, PKA, and PKG: O. Zagoory, et al. ; J. Gen. Physiol. 119, 329 (2002), Application(s): EIA using frog cell lysates, Abstract;

Differential nitric oxide synthase activity, cofactor availability and cGMP accumulation in the central nervous system during anaesthesia: H.F. Galley, et al. ; Br. J. Anaesth. 86, 388 (2001), Application(s): EIA using rat cell lysates, Abstract;

Mechanism underlying diuretic effect of L-NAME at a subpressor dose: M. Liang, et al. ; Ren. Physiol. 281, F414 (2001),Application(s): EIA using rat microdialysate, Abstract; Full Text

The plasma membrane calmodulin-dependent calcium pump: a major regulator of nitric oxide synthase I: K. Schuh, et al. ; J. Cell Biol. 155, 201 (2001), Application(s): EIA using human cell lysates, Abstract;

Role of cGMP versus 20-HETE in the vasodilator response to nitric oxide in rat cerebral arteries: C.W. Sun, et al. ; Am. J. Physiol. Heart Circ. Physiol. 279, H339 (2000), Application(s): EIA using rat cell lysates, Abstract;

Increased cerebrospinal fluid cAMP levels in Alzheimer's disease: M. Martinez, et al. ; Brain Res. 846, 265 (1999),Application(s): EIA using human cerbrospinal fluid, Abstract;

 

 

产品编号 产品名称 产品规格 产品等级
ADI-900-066 Direct cAMP ELISA kit
直接环磷酸腺苷酶联免疫试剂盒		 96 wells –
ADI-901-066 Direct cAMP ELISA kit
直接环磷酸腺苷酶联免疫试剂盒		 5×96 wells –
ADI-900-067 cAMP ELISA kit
环磷酸腺苷酶联免疫试剂盒		 96 wells –
ADI-901-067 cAMP ELISA kit
环磷酸腺苷酶联免疫试剂盒		 5×96 wells –
ADI-900-163 cAMP complete ELISA kit
环磷酸腺苷完全酶联免疫试剂盒		 96 wells –
ADI-901-163 cAMP complete ELISA kit
环磷酸腺苷完全酶联免疫试剂盒		 5×96 wells –
ADI-900-164 cGMP complete ELISA kit
环磷酸腺苷酶完全酶联免疫试剂盒		 96 wells –
ADI-901-164 cGMP complete ELISA kit
环磷酸腺苷酶完全酶联免疫试剂盒		 5×96 wells –
ADI-900-013 cGMP ELISA kit
cGMP ELISA试剂盒		 96 wells –
ADI-901-013 cGMP ELISA kit
cGMP ELISA试剂盒		 5×96 wells –
ADI-900-014 Direct cGMP ELISA kit
直接环磷酸腺苷酶联免疫试剂盒		 96 wells –
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GFP (B-2）抗体 （Alexa Fluor® 405 标记） GFP (B-2) Alexa Fluor® 405

发表于由

GFP (B-2）抗体 （Alexa Fluor® 405 标记）

GFP (B-2) Alexa Fluor® 405

详细描述：
The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous subs

应用范围：WB, IP, IF, FCM, ELISA； 反应种属：； Isotype：mouse monoclonal IgG2a； 标记：AF405。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-9996 AF405 GFP (B-2）抗体 （Alexa Fluor® 405 标记） 100 µg/2 ml 咨询客服