标签归档：quantification

荧光染料ProteOrange Protein Quantification Reagent, 500x

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Cat. # Quantity Price Lead time Buy this product
11210 100 uL –   ProteOrange Protein Quantification Reagent, 500x Sample request
41210 1 mL 195$

Sample request ProteOrange Protein Quantification Reagent, 500x ProteOrange Protein Quantification Reagent, 500x

This reagent is a cost-efficient way to perform high throughput protein quantitation in well plates. This reagent is recommended for quantitating purified protein or antibody samples when performing many experiments daily with the same calibration. The ProteOrange dye has low tolerance to non-ionic detergents, and is not recommended for use with cell lysates containing Triton X-100, sodium deoxycholate. The assay can tolerate up to 0.01% SDS (in final concentration).

The unique structure of the ProteOrange reagent allows accurate detection of proteins in solutions with concentrations between 10 ng/mL and 10 µg/mL . This level of sensitivity is considerably better than that achieved with the BCA method, Bradford assay, Lowry assay, or absorption measurements at 280 nm.

ProteOrange Protein Quantification Reagent, 500x
ProteOrange Protein Quantification Reagent, 500x
ProteOrange Protein Quantification Reagent, 500x

Excitation and emission spectra of ProteOrange complex with protein

ProteOrange Protein Quantification Reagent, 500x

Customers also purchased with this product

QuDye Protein Quantification Reagent, 200x

Highly sensitive and selective reagent for fluorometric quantification of protein concentration, using both fluorescence microplate readers and cuvette-based devices. The dye is highly tolerant to non-protein impurities, except surface acting agents.

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荧光染料QuDye Protein Quantification Reagent, 200x

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lumiprobe荧光染料官网代理商 水溶性荧光染料代理商 脂溶性荧光染料代理商，欢迎访问上海金畔生物科技有限公司官网了解更多信息。

Cat. # Quantity Price Lead time Buy this product
55210 1.5 mL 125$

Bulk inquiry QuDye Protein Quantification Reagent, 200x QuDye Protein Quantification Reagent, 200x

QuDye Protein Quantification Reagent is designed for quantification of protein concentration by a fluorometer in the range of initial protein concentration from 12.5 to 5000 µg/mL. The reagent can be used for quantification of protein concentration with both fluorescence microplate readers and cuvette-based fluorometers, whose measurement range can be wider, depending on the characteristics of the device. Because the reagent selectively binds to SDS-protein micelles, the presence of nucleotides, DNA, RNA, amino acids, salts, reducing agents (2-mercaptoethanol, DTT), and other impurities (except detergents) in the sample does not affect the result of measurement. QuDye Protein Quantification Reagent is quite versatile and allows quantification of the concentration of various proteins with high sensitivity.

This product is a component of QuDye Protein Quantification Kit and is used as an OEM component of kits by other manufacturers.

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H2DCFDA (2′,7′-dichlorodihydrofluorescein diacetate)

A reagent for detecting reactive oxygen species in living cells.

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Mouse Feeder Cell Quantification Kit酶试剂盒Takara

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上海金畔生物科技有限公司代理Takara酶试剂盒全线产品，欢迎访问官网了解更多产品信息。

Mouse Feeder Cell Quantification Kit
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Takara RR290 Mouse Feeder Cell Quantification Kit 100 Rxns Inquire Mouse Feeder Cell Quantification Kit Mouse Feeder Cell Quantification Kit Mouse Feeder Cell Quantification Kit
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■ 制品内容（25 μl × 100次量）
1. TB Green Premix Ex Taq™ II（Tli RNaseH Plus）（2×Conc.） 625 μl × 4
2. Mouse_Mt_primer（10 μM each） 100 μl
3. Mouse_genome_primer(10 μM each) 100 μl
4. Human_primer_Type I（CHR3）（10 μM each） 100 μl
5. Human_primer_Type II（CHR7）（10 μM each） 100 μl
6. Human_primer_Type III（CHR15）（10 μM each） 100 μl
7. dH2O 1 ml×2
8. EASY Dilution（for Real Time PCR） 1 ml×2
9. ROX Reference Dye（50×Conc.）＊ 50 μl
10. ROX Reference Dye II（50×Conc.）＊ 50 μl
11. Human Genomic DNA（100 ng/μl） 20 μl
 
*：用于Applied Biosystems的Real Time PCR仪，用以校正孔与孔之间的荧光信号误差。
◆ 使用ROX Reference Dye 的PCR仪
·StepOnePlus Real-Time PCR System（Thermo Fisher Scientific）
◆ 使用ROX Reference Dye II的PCR仪
·Applied Biosystems 7500 Fast Real-Time PCR System（Thermo Fisher Scientific）
◆ 不使用ROX Reference Dye或ROX Reference Dye II的PCR仪
·Thermal Cycler Dice Real Time System II/Lite (Code No. TP900/TP960/TP700/TP760: 终卖)
 
Limited Use Label License：[M82]
 
■ 制品说明
小鼠饲养细胞可用于维持包括hESC（人胚胎干细胞）及 hiPSC（人诱导多能干细胞）等干细胞的增殖及分化。但对于干细胞的某一种应用如再生医学，去除异种饲养细胞是非常关键的。为了保证干细胞质量，有必要建立残留小鼠细胞的检测体系。
本制品以从hESC及hiPSC等培养干细胞提取的基因组DNA为模板，应用Real Time PCR方法对残留的小鼠饲养细胞来源的基因组DNA进行高灵敏度检测和定量的试剂盒。
本制品使用的引物是在小鼠线粒体（mt）DNA区域设计的Real Time PCR扩增用引物，可高灵敏度地检测小鼠饲养细胞来源的基因组DNA。现已知mtDNA拷贝数依据细胞种类、细胞株及分化状态不同而不同，使用的小鼠饲养细胞批次间也可能有差异，因此在试剂盒中附带了可检测小鼠mtDNA拷贝数的引物，用于小鼠饲养细胞批次间的校正。＊
*：使用同一个标准曲线对多批次小鼠饲养细胞来源的基因组DNA定量时，需确认各批次间的mtDNA拷贝数无差异。当小鼠饲养细胞每个批次都制作标准曲线时，则不需要校正。
 
■ 保 存
TB Green Premix Ex Taq II（Tli RNaseH Plus）
4℃可保存6个月。
避光保存。避免污染。
※长期保存时，请于-20℃保存。产品融解后请于4℃保存，并在6个月内用完。
其他组分
-20℃保存
 
 

页面更新：2022-06-24 13:03:04