组成品

状态

包装

（A）抗体包被96孔板

清洗后使用

96 wells（8×12）/1个

（B）标准溶液（100 ng/mL）

稀释后使用

200 μL/1瓶

（C）测定用缓冲液

直接使用

60 mL/1瓶

（D）生物素结合抗GH抗体

稀释后使用

100 μL/1瓶

（E）过氧化物酶·抗生素结合物

稀释后使用

100 μL/1瓶

（F）显色液（TMB）

直接使用

12 mL/1瓶

（G）样本稀释用缓冲液

直接使用

12 mL/1瓶

（H）终止液（1M H₂SO₄）

※小心轻放

直接使用

12 mL/1瓶

（I）浓缩清洗液（10×）

稀释后使用

100 mL/1瓶

孔板密封膜

4个

产品说明书

1本

样本

A

B

1

8.74

1.15

2

8.78

1.03

3

8.79

1.07

4

8.68

1.06

5

8.03

1.03

6

8.27

1.13

7

7.94

1.07

8

7.97

1.05

Mean

8.40

1.07

SD

0.39

0.04

CV（%）

4.6

3.9

测定日/样本

E

F

G

0天

5.01

1.25

0.328

1天

5.01

1.24

0.329

2天

5.00

1.27

0.347

3天

5.00

1.28

0.337

Mean

5.00

1.26

0.335

SD

0.0019

0.016

0.0088

CV（%）

0.038

1.3

2.6

添加量

实测值

回收量

回收率（%）

0.00

1.29

—

—

0.584

1.89

0.601

103

1.17

2.48

1.19

102

1.75

3.01

1.73

98.5

添加量

实测值

回收量

回收率（%）

0.00

2.23

—

—

1.36

3.59

1.35

100

3.39

5.60

3.36

99.2

4.07

6.21

3.97

97.7

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Easy detection of hormone secretion from LβT2 cells by using Gaussia luciferase.Satou K, Mochimaru Y, Nakakura T, Kusada T, Negishi J, Musha S, Yoshimura N, Kato Y, Tomura H.J Reprod Dev. 2017 Apr 21;63(2):199-204.

 2.

α-Lipoic acid potentially targets AMP-activated protein kinase and energy production in the fetal brain to ameliorate dioxin-produced attenuation in fetal steroidogenesis.Takeda T, Matsuo Y, Nishida K, Fujiki A, Hattori Y, Koga T, Ishii Y, Yamada H.J Toxicol Sci. 2017;42(1):13-23.

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Kisspeptin expression is decreased in the arcuate nucleus of hypothyroid female rats with irregular estrus cycles.Tomori Y, Takumi K, Iijima N, Takai S, Ozawa H.Neurosci Res. 2016 Nov 27.

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Increase of kisspeptin-positive cells in the hypothalamus of a rat model of polycystic ovary syndrome. Kondo M, Osuka S, Iwase A, Nakahara T, Saito A, Bayasula, Nakamura T, Goto M, Kotani T, Kikkawa F Metab Brain Dis. 2016 Jun;31(3):673-81.

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The combined fixed-dose antituberculous drugs alter some reproductive functions with oxidative stress involvement in wistar rats O. Awodele, B.Pharm M.Sc MPH PhD D.Sc FPCPharm FASIa, A.A. Momoha, N.A. Awololac, O.E. Kalea, b, W.O. Okunowod Toxicology Reports,Vol.3, 2016, Pages 620–627

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Hormone Inhibition During Mini-Puberty and Testicular Function in Male Rats. Chen L, Wang R, Wang W, Lu W, Xiao Y, Wang D, Dong Z. Int J Endocrinol Metab. 2015 Oct 3;13(4):e25465

 7.

Increase of kisspeptin-positive cells in the hypothalamus of a rat model of polycystic ovary syndrome. Kondo M, Osuka S, Iwase A, Nakahara T, Saito A, Bayasula, Nakamura T, Goto M, Kotani T, Kikkawa F Metab Brain Dis. 2016 Feb 11. [Epub ahead of print

 8.

Oleuropein supplementation increases urinary noradrenaline and testicular testosterone levels and decreases plasma corticosterone level in rats fed high-protein diet
Oi-Kano Y., Kawada T., Watanabe T., Koyama F., Watanabe K., Senbongi R., Iwai K.The Journal of Nutritional Biochemistry, Vol.24(5), p887-893, May 2013.

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Multiple Cholinergic Signaling Pathways in Pituitary Gonadotrophs Zemkova H., Kucka M., Bjelobaba IV., Tomi M. and Stojilkovic SS. Endocrinology, Vol.154(1), p421-433, Jan 2013.

10.

ERα/E2 signaling suppresses the expression of steroidogenic enzyme genes via cross-talk with orphan nuclear receptor Nur77 in the testes S.-Y. Lee., E. Park., S.-C. Kim., R.-S. Ahn., C. Ko., K. Lee. Molecular and Cellular Endocrinology, 2012.

11.

Gonadotropin-Inhibitory Hormone Inhibits GnRH-Induced Gonadotropin Subunit Gene Transcriptions by Inhibiting AC/cAMP/PKA-Dependent ERK Pathway in LβT2 Cells Son YL., Ubuka T., Millar RP., Kanasaki H. and Tsutsui K. Endocrinology, Vol.153(5), p2332-2343, May 2012.

12.

Anti-androgenic effects of S-40542, a novel non-steroidal selective androgen receptor modulator (SARM) for the treatment of benign prostatic hyperplasia H. Nejishima., N. Yamamoto., M. Suzuki., K. Furuya., N. Nagata., S. Yamada. The Prostate published online:16 MAR 2012

13.

Gonadotropin-Inhibitory Hormone Inhibits GnRH-Induced Gonadotropin Subunit Gene Transcriptions by Inhibiting AC/cAMP/PKA-Dependent ERK Pathway in LβT2 Cells Y. L. Son., T. Ubuka., R. P. Millar., H. Kanasaki., and K. Tsutsui. Endocrinology, Feb 28, 2012

14.

Neonatal immune challenge alters reproductive development in the female rat L. Sominsky., C.L. Meehan., A.K. Walker., L. Bobrovskaya., E. A. McLaughlin., D. M. Hodgson. Hormones and Behavior, 2012.