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Caged ATP
Caged ATP
- 細胞内蛍光プローブ
ケージド試薬: ATP
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製品コードCC01 Caged ATP
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CAS番号67030-27-7(free acid)
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化学名P3-[1-(2-Nitrophenyl)ethyl]adenosine-5′-triphosphate，trisodium salt
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分子式・分子量C18H20N6Na3O15P3=722.27
| 容 量 | メーカー希望 小売価格 |
富士フイルム 和光純薬 |
|---|---|---|
| 1 mg | ￥21,600 | 345-05503 |
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マニュアル
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プロトコル
細胞内目的部位にATP を局部添加したい 
参考文献
1) J. H. Kaplan, B. F. Iii and J. F. Hoffman, "Rapid Photolytic Release of Adenosine 5'-triphosphate from a Protected Analogue: Utilization by the Na: K Pump of Human Red Blood Cell Ghosts", Biochemistry, 1978, 17, 1929.
2) E. Ohtsuka, H. Uemura, T. Doi, T. Miyake, S. Nishikawa and M. Ikehara, "A New Method for 3'Labelling of Polyribonucleotides by Phosphorylation with RNA Ligase and Its Application to the 3'-Modification for Joining Reactions", Nucleic Acids Res., 1979, 6, 443.
3) J. A. McCray, L. Herbette, T. Kihara and D. R. Trentham, "A New Approach to Time-resolved Studies of ATP-requiring Biological Systems: Laser Flash Photolysis Caged ATP", Proc. Natl. Acad. Sci. USA, 1980, 77, 7237.
4) Y. E. Goldman, M. G. Hibberd, J. A. McCray and D. R. Trentham, "Relaxation of Muscle Fibres by Photolysis of Caged ATP", Nature, 1982, 300, 701.
5) D. H. Pierce, A. Scarpa, M. R. Topp and J. K. Blasie, "Kinetics of Calcium Uptake by Isolated Sarcoplasmic Reticulum Vesicles Using Flash Photolysis of Caged Adenosine 5'-Triphoshate", Biochemistry, 1983, 22, 5254.
6) 杉晴夫, "アクチンとミオシンの構造と両者間の相互作用", 生体の科学, 1983, 34, 334.
7) B. F. Iii, "Na+ Movement in a Single Turnover of the Na Pump", Proc. Natl. Acad. Sci. USA, 1984, 81, 5310.
8) Y. E. Goldman, "Laser Pulsed Release of ATP and Other Optical Methods in the Study of Muscle Contraction", Soc. Gen. Physiol. Ser., 1986, 40, 397.
9) J. W. Walker, G. P. Reid, J. A. McCray and D. R. Trentham, "Photolabile 1-(2-Nitrophenyl)ethyl Phosphate Esters of Adenine Nucleotide Analogues. Synthesis and Mechanism of Photolysis", J. Am. Chem. Soc., 1988, 110, 7170.
10) J. W. Walker, G. P. Reid and D. R. Trentham, "[16] Synthesis and Properties of Caged Nucleotides", Methods in Enzymol., 1989, 172, 288.
11) K. Horiuti, T. Sakoda, M. Takei and K. Yamada, "Effects of Ethylene Glycol on the Kinetics of Contraction on Flash Photolysis of Caged ATP in Rat Psoas Muscle Fibres", J. Muscle. Res. and Cell Motility, 1992, 13, 199.
12) Z. Lu, R. L. Moss and J. W. Walker, "Tension Transients Initiated by Photogenerations of MgADP in Skinned Skeletal Muscle Fibers", J. Gen. Physiol., 1993, 101, 867.
取扱条件
| 性状： | 本品は、白色粉末で水に溶ける。 |
|---|---|
| 純度(HPLC)： | 98.0％ 以上 |
| ATP含量(HPLC)： | 0.1％ 以下 |
| NMRスペクトル： | 試験適合 |
| 1.保存方法：冷凍,遮光 |
