标签归档：胚层

人 iPS细胞向定型内胚层细胞定向分化系统酶试剂盒Takara

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人 iPS细胞向定型内胚层细胞定向分化系统
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Cellartis Y30035 Cellartis® Definitive Endoderm Differentiation Kit with DEF-CS™ Culture System 1 Kit ￥5,743 人 iPS细胞向定型内胚层细胞定向分化系统 人 iPS细胞向定型内胚层细胞定向分化系统 人 iPS细胞向定型内胚层细胞定向分化系统
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Cellartis定向分化获取内胚层细胞试剂盒
(Cellartis Definitive Endoderm Differentiation Kit)
The Cellartis Definitive Endoderm Differentiation Kit用于定向分化hiPS获取definitive endoderm (DE) cells。本产品包含所有必需的培养基和coating材料。本产品还包括新一代的Cellartis DEF-CS 100 Culture System用于起始hiPS的维持培养用。使用本产品获得的DE细胞，可以作为起始材料进行定向分化至内胚层来源的细胞类型(如肝细胞，胰腺细胞)。Cellartis也提供分化DE细胞获取肝细胞的试剂盒（Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050)；Cellartis也提供诱导分化hiPS获取肝细胞的试剂盒（Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055)。
 
■ 产品特点
· Efficient differentiation—generates >80% SOX17-positive cells
· Robust protocol—successful definitive endoderm (DE) cell generation from more than 25 different hiPS cell lines
· Complete kit—media and ready-to-use coating for a seven-day protocol to reproducibly differentiate hiPS cells into DE cells
· Start with a homogeneous population of hiPS cells—kit includes the Cellartis DEF-CS 100 Culture System to maintain undifferentiated cells prior to the start of DE cell differentiation
 
 
■ 产品详情请点击：人 iPS细胞向定型内胚层细胞定向分化系统
 
人 iPS细胞向定型内胚层细胞定向分化系统
mRNA expression profiles of definitive endoderm cells being generated from two hiPS cell lines (ChiPSC18 and P11025) using the Cellartis Definitive Endoderm Differentiation Kit with DEF-CS Culture Medium. These graphs track the relative temporal mRNA expression levels of various markers during differentiation from hiPS cells (starting on Day 0) into DE cells (by Day 7), as measured by RT-qPCR.
 
 

页面更新：2019-12-25 09:40:07

人定型内胚层细胞向肝脏细胞定向分化系统酶试剂盒Takara

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上海金畔生物科技有限公司代理Takara酶试剂盒全线产品，欢迎访问官网了解更多产品信息。

人定型内胚层细胞向肝脏细胞定向分化系统
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Cellartis Y30050 Cellartis® Hepatocyte Differentiation Kit 1 Kit ￥8,072 人定型内胚层细胞向肝脏细胞定向分化系统 人定型内胚层细胞向肝脏细胞定向分化系统 人定型内胚层细胞向肝脏细胞定向分化系统
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Cellartis分化获取肝细胞试剂盒
(Cellartis Hepatocyte Differentiation Kit)
The Cellartis Hepatocyte Differentiation Kit用于诱导内胚层细胞（definitive endoderm (DE)）获取肝细胞。通过使用本产品获取的肝细胞表现出符合预期的代谢酶表达，从而可以用于药物代谢研究和毒理学筛选。本产品包含足够的维持培养基用于正常为期11天的实验。
The Cellartis Hepatocyte Differentiation Kit可以对广泛来源的DE细胞进行定向分化：客户自己的DE细胞，或是Cellartis提供的标准化DE细胞，或是客户利用Cellartis Definitive Endoderm Differentiation Kit获得的DE细胞。据估计，现在已经有28种发源于人类干细胞的DE细胞被成功测试可以使用本产品。特别地，如果客户是用于大规模、要求持续稳定获得肝细胞的相关用途，Cellartis推荐Definitive Endoderm Cells (from ChiPSC18)，用作起始DE细胞材料，或是用作阳性对照品。如果客户要求的DE是来自于自有的样本材料（patient-specific, or disease-specific），建议使用Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055)。
 
■ 产品特点
· Complete kit—includes four different media sets and a ready-to-use coating, sufficient for
   23-day protocol for differentiating definitive endoderm (DE) cells into hepatocytes
· Robust protocol—tested with more than 25 different stem cell lines
· Efficient differentiation—yields >90% pure hepatocytes
 
■ 关联产品
 
Code No. Product Size
Y10040 Cellartis® Definitive Endoderm Cells (from ChiPSC18) 1 Vial
Y30051 Cellartis® Hepatocyte Maintenance Medium 100 ml
 
■ 产品详情请点击：人定型内胚层细胞向肝脏细胞定向分化系统
 
人定型内胚层细胞向肝脏细胞定向分化系统
Definitive endoderm (DE) cells were differentiated into hepatocytes using the Cellartis Hepatocyte Differentiation Kit. The first field of view, labeled “Definitive Endoderm Day 7,” shows DE cells seven days after differentiation from human pluripotent cells. These DE cells became the starting material for the differentiation kit. The remaining progression from DE cells to hepatocytes shows the expected morphology over a 14-day time period. The morphological features present in the last field of view, labeled “Hepatocyte Day 21+,” continue stably past Day 21.
 
 

页面更新：2019-12-25 09:40:58

人iPS细胞来源定型内胚层细胞酶试剂盒Takara

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上海金畔生物科技有限公司代理Takara酶试剂盒全线产品，欢迎访问官网了解更多产品信息。

人iPS细胞来源定型内胚层细胞
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Cellartis Y10040 Cellartis® Definitive Endoderm Cells (from ChiPSC18) 1 Vial ￥8,387 人iPS细胞来源定型内胚层细胞 人iPS细胞来源定型内胚层细胞 人iPS细胞来源定型内胚层细胞
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Cellartis定型内胚层干细胞
[Cellartis Definitive Endoderm Cells (from ChiPSC18)]
Cellartis定型内胚层干细胞（DE cell）是从hiPS细胞株ChiPSC18经体外诱导分化得来。该产品中，大于90%的细胞显示表达SOX17蛋白。该产品为低温冷冻保存的细胞悬浮形式。本产品适合用于进一步诱导分化成为肝脏细胞类型或是胰脏细胞类型。特别地，Cellartis的肝脏细胞诱导分化试剂盒（Cellartis Hepatocyte Differentiation Kit，Cat. No. Y30050）适合用于DE向肝脏细胞类型的诱导分化工作。
 
■ 产品特点
· Definitive endoderm cells with >90% SOX17-positive cells
· Can be used for generating further-specified cells of endodermal origin, such as hepatocytes or pancreatic endoderm
 
■ 关联产品
Code No. Product Size
Y10052 Cellartis® HEP Coat 3 ml
Y30012 Cellartis® DEF-CS™ 500 COAT-1 4 ml
T303 iMatrix-511 2 × 175 μg
T304 iMatrix-511 6 × 175 μg
Y30050 Cellartis® Hepatocyte Differentiation Kit 1 Kit
 
■ 产品详情请点击：人iPS细胞来源定型内胚层细胞
 
人iPS细胞来源定型内胚层细胞
Immunocytochemistry of Cellartis Definitive Endoderm Cells (from ChiPSC18). Cells were thawed, cultured for two days, fixed, and stained for the pluripotency marker OCT4, the definitive endoderm marker SOX17, and the nuclear marker DAPI. Results show that more than 90% of the cell population are definitive endoderm cells and have lost pluripotency.
 
 
 

页面更新：2019-12-25 09:45:34