总细胞数（cells/vial）

活细胞数（cells/vial）

活细胞比例（%）

活细胞数比
（假设未经过冻存的活细胞数为1）

①

未经冻保

1.74×10⁶

1.65×10⁶

95

1

②

脑组织冻存液（本品）

1.21×10⁶

1.11×10⁶

92

0.67

③

本公司的冻存液（通用动物细胞用）

（含有血清成分）

8.34×10⁵

7.29×10⁵

87

0.44

④

其他公司的冻存液（通用动物细胞用）

（含有血清成分）

3.40×10⁵

2.91×10⁵

86

0.18

产品编号

产品名称

规格

包装

029-19161

Brain Tissue Freezing Medium
脑组织冻存液

细胞培养用

1 mL×10

产品编号

产品名称

规格

包装

148-09671

Neuron Culture Medium
神经细胞用培养基

细胞培养用

100 mL

产品编号

产品名称

规格

包装

291-78001

Neuron Dissociation Solutions
神经细胞用分散液

细胞培养用

4 set

297-78101

Neuron Dissociation Solutions S
神经细胞用分散液S

细胞培养用

10 set

产品编号

产品名称

规格

包装

148-09615

NS Basal Medium
NS基础培养基

细胞培养用

500 mL

146-09351

NS Supplement(×50)

细胞培养用

10 mL

149-09721

NS Supplement without Insulin(×50)
NS 添加剂（不含胰岛素）（×50）

细胞培养用

10 mL

145-09723

50 mL

142-09691

NS Supplement without Vitamin A(×50)
NS 添加剂（不含维生素A）（×50）

细胞培养用

10 mL

141-09041

N2 Supplement with Transferrin(Apo)(×100)
N2神经细胞生长添加剂含转铁蛋白（Apo）（×100）

细胞培养用

5 mL

141-08941

N2 Supplement with Transferrin(Holo)(×100)
N2神经细胞生长添加剂含转铁蛋白（Holo）（×100）

细胞培养用

5 mL

073-05391

200 mmol/L L-Glutamine Solution (×100)
200 mmol/L 谷氨酸溶液（×100）

细胞培养用

100 mL

产品编号

产品名称

等级

包装

039-23511

CultureSure Freezing Medium
CultureSure无血清通用细胞冻存液

细胞培养用

100 mL

产品编号

产品名称

等级

包装

195-16031

StemSure Freezing Medium
StemSure干细胞冻存液

细胞培养用

100 mL

1.

Kusakisako, K., Masatani, T., Yada, Y., Talactac, M. R., Hernandez, E. P., Maeda, H., … & Tanaka, T. (2016). Improvement of the cryopreservation method for the Babesia gibsoni parasite by using commercial freezing media. Parasitology international, 65(5), 532-535. 全文

2.

Shirozu, T., Soga, A., & Fukumoto, S. (2020). Identification and validation of a commercial cryopreservation medium for the practical preservation of Dirofilaria immitis microfilaria. 全文

3.

Lai, Y. C., Ushio, N., Rahman, M. M., Katanoda, Y., Ogihara, K., Naya, Y., … & Sunaga, T. (2018). Aberrant expression of microRNAs and the miR‐1/MET pathway in canine hepatocellular carcinoma. Veterinary and comparative oncology, 16(2), 288-296. 全文

4.

Rahman, M. M., Lai, Y. C., Husna, A. A., Chen, H. W., Tanaka, Y., Kawaguchi, H., … & Miura, N. (2019). Aberrantly expressed snoRNA, snRNA, piRNA and tRFs in canine melanoma. Veterinary and Comparative Oncology.  全文

BAMBANKER^® 是一种无血清细胞冻存液。可在 -80℃ 长期保存细胞（肿瘤细胞和常规细胞）。

P3U1

（小鼠骨髓瘤细胞系）

K562

（人白血病细胞系）

human γδT cells

（人γδT细胞）

 Daudi

（人 B细胞系）

PC12

（大鼠源肾上腺嗜铬细胞瘤）

human B cell line 

（人B细胞系）

OKT4

（小鼠杂交瘤细胞）

B细胞系

(猴）

3T3‐L1（小鼠前脂肪细胞系）

A431（人扁平上皮癌细胞系）

BAEC（牛主动脉血管内皮细胞系）

Balb/3T3（小鼠成纤维细胞系）

C2C12（小鼠骨骼肌细胞系）

 Daudi（人B细胞系）

ECV304（人脐静脉内皮细胞系）

H295R（肾上腺皮质细胞）

HEK293（人胚胎肾细胞系）

HEK293T（人胚胎肾细胞系）

HeLa（人子宫颈癌细胞系）

HeLa S3（人子宫颈癌细胞系）

HepG2（人肝癌细胞系）

HFF（人正常成纤维细胞系）

Huh7（人肝癌细胞系）

Jurkat（人白血病T细胞系）

K562（人慢性骨髓性白血病细胞系）

KATOIII （人胃癌上皮细胞系）

KLM‐1（人胰腺癌细胞系）

MDCK（犬肾小管上皮细胞系）

MEF（小鼠胚胎成纤维细胞）

NIH3T3（小鼠胚胎皮肤细胞）

OKT4（小鼠杂交瘤细胞）

OP9（小鼠骨髓基质细胞）

P3U1（小鼠骨髓瘤细胞系）

PANC‐1（人胰腺癌细胞系）

PC12（大鼠源肾上腺嗜铬细胞瘤）

RPE（人视网膜上皮细胞系）

SNL（小鼠胚胎成纤维细胞）

TSU‐Pr1（人前列腺癌细胞系）

Vero（非洲绿猴肾细胞系）

human γδT cells （人γδT细胞）

human B cell line （人B细胞系）

HS-RMS-2

（多形性横纹肌肉瘤细胞系）

LNCaP clone FGC

（人前列腺癌细胞）

MEF-BL/6-1

（小鼠胚胎成纤维细胞）

猴B细胞系

人外周血活化淋巴细胞

永生化人肌肉细胞

小鼠脾脏活化淋巴细胞

小鼠ES细胞系

人胃上皮细胞

BAMBANKER^® 无血清细胞冻存液

Wako BAMBANKER冻存液（新手册）

细胞种类列举

BAMBANKER细胞冻存液

–冻存解冻步骤说明

（终端）.pdf

１.

Q：为什么我使用了 BAMBANKER^® 来保存细胞，但是存活率依然不高？

A：请冻存前确保细胞处于生长对数期，并且冻存时细胞数目控制在 5×10⁵~1×10⁷/mL 冻存液。

2.

Q：我们实验室已经有固定的冻存程序了，换了你们的 BAMBANKER^® 可以还继续用原来程序降温的方法冻存吗？

A：虽然本产品可以无需程序降温冻存细胞，但如果通过程序降温盒等适当控制了温度下降的速度，效果更佳。

3.

Q：哪些细胞株（系）适合使用BAMBANKER^® 来进行细胞冷冻保存？

A：几乎所有细胞株（系）都可以使用 BAMBANKER^® 进行冻存。对于较为宝贵的 ES/iPS 细胞的保存尤其适用。官网上所列举的细胞系均已经过测试验证。

但也并不排除可能有某些细胞株是不适合使用 BAMBANER^® 来进行冻存的，用户在没有确认是否可使用时，建议在进行正式细胞冻存之前先进行预实验。

4.

Q：无血清冻存液相比传统含血清冻存液有什么优势？

A：无血清冻存液因不含有动物血清，质量更稳定，批间差小；同时未知生物成分或感染性物质污染细胞的几率也极低，尤其对于 ES/iPS 细胞等有可能用于再生医疗的细胞安全得以严格保障；可以直接冻存无血清培养的细胞，免去无血清再驯化的步骤；另外 BAMBANKER^® 无血清细胞冻存液不需要像传统的血清冻存液需要程序降温，减少了用户的繁琐操作，节省了时间。

5.

Q：BAMBANKER^® 在冷冻保存细胞的过程中起什么作用？

A：BAMBANKER^® 无血清细胞冻存液使用了 DMSO 等作为保护剂，在冻存细胞时能以１℃/min 左右的温度下降而逐渐冻结，在此过程中，细胞内的水分子被置换成冻结保护剂，抑制胞内和细胞周边的冰晶的形成，防止细胞膜和细胞器结构损伤，防止蛋白变质。

6.

Q：未使用的BAMBANKER^® 无血清细胞冻存液应该如何保存？

A：2-10℃ 避光保存。开封后尽快使用。请注意保质期为自生产日期起 24 个月。

7.

Q：BAMBANKER^® 能否使用于医疗领域?

A：BAMBANKER^® 仅供科研使用，不能使用于人体或医疗领域。

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