水溶性lumiprobe荧光染料63390 Sulfo-Cyanine5 carboxylic acid 100 mg 水溶性lumiprobe荧光染料Sulfo-Cyanine5 羧酸 100 mg 1990

高性价比茴香油杂质标准品哪里找？—上海金畔生物< ?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" />

上海金畔生物科技有限公司是一家专业经营标准物质、标准品、化学试剂及相关技术服务创新型高科技企业。

     自公司成立以来，一直以“客户满意“为公司核心价值观，产品主要应用于制药、生物、食品、环境、材料和农业等领域。凭借世界一流的产品和服务，金畔生物与广大客户建立了长期稳定的战略合作关系，被众多企业和科研机构认定为“指定供应商”，得到了政府部门的关怀和有力支持。本着始终拥有的创业激情和服务热忱，金畔生物已成长为我国重要的标准物质和标准品领域集成服务的领导者、中国最大的标准物质/标准品供应商之一。

    金畔生物集后发优势与众多国际一流品牌合作，并陆续成为他们在中国区的总代理或者一级代理，现合作的优质供应商有：美国AccuStandard、APSC、MPBio、Sigma-Aldrich、NIST，爱尔兰Reagecon、Megazyme，英国LGC、Ultra，Iduron、日本和光（WAKO）、Shodex，德国Dr.E、PSS 等。同时，还提供美国USP标准物质、欧洲药典标准物质EDQM、加拿大TRC标准物质等。

上海金畔生物提供高性价茴香油杂质标准品，为您的检测分析提供强有力的支持！

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上海金畔生物科技有限公司 www.jinpanbio.com

文章号：1194JP1194

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上海金畔生物科技有限公司

文章号4886JP4886

高性价比聚丙交酯GPC分子量测定标准品哪里找？—-上海金畔生物

     上海金畔生物科技有限公司是一家专业经营标准物质、标准品、化学试剂及相关技术服务创新型高科技企业。
     自公司成立以来，一直以”客户满意”为公司核心价值观，产品主要应用于制药、生物、食品、环境、材料和农业等领域。凭借世界一流的产品和服务，金畔生物与广大客户建立了长期稳定的战略合作关系，被众多企业和科研机构认定为“指定供应商”，得到了政府部门的关怀和有力支持。本着始终拥有的创业激情和服务热忱，金畔生物已成长为我国重要的标准物质和标准品领域集成服务的领导者、中国最大的标准物质/标准品供应商之一。
     金畔生物集后发优势与众多国际一流品牌合作，并陆续成为他们在中国区的总代理或者一级代理，现合作的优质供应商有：美国AccuStandard、APSC、MPBio、Sigma-Aldrich、NIST，爱尔兰Reagecon、Megazyme，英国LGC、Ultra，Iduron、日本和光（WAKO）、Shodex，德国Dr.E、PSS 等。同时，还提供美国USP标准物质、欧洲药典标准物质EDQM、加拿大TRC标准物质等。

    上海金畔生物提供高性价比聚丙交酯GPC分子量测定标准品，为您的检测分析提供强有力的支持！

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种属反应性

产品货号：ZZK-K-ACHDF

产品品名：可吸收糖类和膳食纤维检测试剂盒

英文品名：Available Carbohydrates/Dietary Fibre Assay Kit

规格型号：100 assays of each component

An integrated procedure for the measurement of available carbohydratesand dietary fibre in cereal products, fruit and vegetables and food products.

INTRODUCTION:

A need for information on the carbohydrate composition of foods fordiabetics prompted McCance and Lawrence1 to attempt to measure this togain results that would be of biological significance. They divided thecarbohydrates in foods into two broad groups, ‘available’ and‘unavailable’. The available carbohydrates, that is sugar plus starch,were defined as those that are digested and absorbed by the human smallintestine and which are glucogenic. The unavailable carbohydrates weredefined as those that are not digested by the endogenous secretions ofthe human digestive tract. These are now generally referred to asdietary fibre.

The concept of ‘available’ and ‘unavailable’ carbohydrates drewattention to the fact that some carbohydrates are not digested andabsorbed in the small intestine but rather reach the large bowel wherethey are fermented. In the FAO/WHO report on “Carbohydrates in HumanNutrition”2, it was stated that these terms can be misleading since theso-called ‘unavailable’ carbohydrate can, in fact, provide energy to thebody through fermentation. This committee recommended use of the term‘glycemic’ (i.e. providing carbohydrate for metabolism) to replace‘available’. However, this term has not gained widespread acceptance,possibly because there is some confusion with the well recognised term‘glycemic index’. For this reason, we have decided to continue using theterm ‘available carbohydrates’ for carbohydrates that are digested andabsorbed by the human small intestine; these include D-glucose,

D-fructose, sucrose, maltodextrins, non-resistant starch and the

D-glucose component of lactose; all measured as D-glucose plus

D-fructose following enzymic hydrolysis.

Dietary fibre is a mixture of complex organic substances including arange of hydrophilic compounds such as soluble and insolublepolysaccharides and non-digestable oligosaccharides as well asnon-swellable, more or less hydrophobic compounds such as cutins,suberins and lignins. The procedures for the determination of totaldietary fibre as outlined in this booklet are based on the methods ofLee et al.3 and Prosky et al.4 (AOAC Official Method 991.43, AACC Method32-07). These methods do not measure non-digestible oligosaccharides, asthese remain soluble in the ethanolic solution used to precipitate highmolecular weight dietary fibre components.

KITS:

Kits suitable for performing 100 assays of each are available fromMegazyme. The kits contain the full assay method plus:

Bottle 1: Buffer (11 mL, pH 7.6) plus sodium azide(0.02% w/v) as apreservative.Stable for > 3 years at 4°C.

Bottle 2: NADP+ plus ATP.Stable for> 5 years at -20°C.

Bottle 3: Thermostable α-amylase (6 mL, 3,000 U/mL)(no. E-BLAAM).Stablefor > 3 years at 4°C.

Bottle 4: Protease (10 mL, ~ 350 U/mL)(no. E-BSPRT).Stable for > 3years at -20°C.

Bottle 5: Amyloglucosidase (20 mL, ~ 3,300 U/mL)(no. E-AMGDF).Stablefor > 3 years at 4°C.

Bottle 6: Sucrase plus β-galactosidase, freeze-dried powder.Stable for> 3 years at -20°C.

Bottle 7: Hexokinase plus glucose-6-phosphate dehydrogenase suspension,2.25 mL.Stable for> 3 years at 4°C.

Bottle 8: Phosphoglucose isomerase suspension (2.25 mL). Stable for> 3 years at 4°C.

Bottle 9: D-Glucose plus D-fructose standard solution(5 mL, 0.2 mg/mLof each sugar).Stable for > 3 years at room temperature.

EQUIPMENT (RECOMMENDED):

1. Duran? bottles (250 mL volume).

2. Fritted crucible, Corning No. 36060 Büchner, fritted disk, Pyrex

60 mL, pore size, coarse, ASTM 40-60 μm, or equivalent. Prepare as follows:

a. Ash overnight at 525°C in muffle furnace.

b. Remove Celite and ash material by using a vacuum.

c. Soak in 2% (v/v) Micro cleaning solution (reagent 6) at roomtemperature for 1 h.

d. Rinse crucibles with water and then deionised water.

e. For final rinse, use 15 mL acetone and air dry.

f. Add approximately 1.0 g Celite to dried crucibles and dry at 130°Cto constant weight.

g. Cool crucible in desiccator for approximately 1 h and record weightof crucible containing Celite.

3. Filtering flask, heavy-walled, with 1-L side arm.

4. Rubber ring adaptors for use on filtering flasks.

5. Vacuum source: vacuum pump or aspirator with regulator capable ofregulating vacuum.

6. Water bath, shaking, large-capacity (20-24 L) with covers; capableof maintaining temperature of 100°C; equipped with automatic timers foron-off operation.

7. Balance, 0.1 mg accuracy.

8. Ovens, two, mechanical convection, set at 103 ± 2°C and

130 ± 3°C.

9. Desiccator, airtight, with SiO2 or equivalent desiccant. Desiccantdried biweekly overnight in 130°C oven.

10. pH meter.

11. Pipettors and tips, 50-200 μL and 5 mL capacity.

12. Dispensers

a. 15 ± 0.5 mL for 78% (v/v) EtOH, 95% ethanol, and acetone.

b. 40 ± 0.5 mL for buffer.

13. Measuring cylinder, 500 mL.

14. Magnetic stirrers and stirring bars.

15. Rubber spatulas.

16. Muffle furnace, 525 ± 5°C.

17. Microfuge, capable of 12,000 g.

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金畔，甄心倾听您每一个标准！

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上海金畔生物科技有限公司

文章号：=2267JP2267

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上海金畔生物科技有限公司

文章号4168JP4168

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上海金畔生物科技有限公司

文章号2931JP2931

whatman 3MM滤纸的应用
whatman 3MM滤纸的应用 3030-861 3MM CHR 20X20CM 100/PK
whatman 3MM滤纸的应用—杂交筛选——cDNA序列作为探针
1．如上述将文库铺平板，噬菌斑杂交实验用E.coli宿主菌株Y1088。
2．噬菌斑于42℃生长过夜。
3．从温箱取出平板，置4℃冷却至少1h。
4．膜剥离：首先，用软铅笔在干的硝酸纤维素滤膜上给准备影印的平板编号。其次，从平板的中央开始向边缘小心将滤膜铺在菌台上。当滤膜完全变湿后（颜色变灰），再等30s。同时，用针头在平板上标出滤膜的3个不对称位置。小心将滤膜从平板上剥离，接触面向上放在一张干净的Whatman 3MM滤纸上。在进行下一步骤之前，所有的平板重复步骤4。
5．将滤膜接触面向上漂浮在变性液（0.5 mol/L NaOH; 1.5 mol/LNaCl）中5min。
6．中和5min。
7．用2×SSC洗涤5min。
8．将滤膜放在一张新的Whatman3MM滤纸上，用铅箔将滤膜和滤纸一起包住。
9．在真空炉中80℃烘烤2h。
10．用6×SSC预先短暂地湿润滤膜，然后用预洗液（50 mmol/LTris-Cl pH8.0; 1mol/L NaCl; 1mmol/L EDTA; 0.1%SDS）于68℃预洗涤至少1h。用一个盘子放在水浴摇床上。
11．将25张滤膜转移到一个装有50ml杂交液（5×SSPE；1×Denhardt’s液；100μg/ml cT-DNA；0.1％SDS）的贮存罐内（直径至少90mm），68℃预杂交2h。
12．对于每ml杂交液，2倍的105至1×106cpm的双链探针经沸水浴变性10min后，迅速放冰上冷却。
13．变性探针立即加到预杂交混合液中。
14．在封口的贮存罐内于68℃杂交过夜，不断地晃动以防滤膜粘在一起。
15．杂交结束后，滤膜用2×SSC，0.01％SDS于室温洗涤3次约1h。
16．将潮湿（防止干燥）的硝酸纤维素滤膜放在一张硬纸或X光片上，用放射性墨水在针头标签上作标记。
17．滤膜用塑料包装袋（如Saran）包住，然后加上增感屏在X光片上于－70℃曝光过夜。

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Amresco 0512-2.5KG POTASSIUM IODIDE 碘化钾

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LGC N-乙酰色氨酸杂质标准品–上海金畔生物

上海金畔生物提供LGC N-乙酰色氨酸杂质标准品，为您的检测分析提供强有力的支持！

上海金畔生物科技有限公司

文章号6009JP6009

水溶性荧光染料叠氮 F1330 Sulfo-Cyanine3 azide 100 mg 水溶性荧光染料叠氮 Sulfo-Cyanine3 叠氮基 100 mg 1990