Buccutite 聚HRP抗体偶联试剂盒
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货号 |
5519 |
存储条件 |
在2-8度冷藏保存, 避免光照 |
| 规格 |
2×50 ug Labelings |
价格 |
5940 |
| Ex (nm) |
|
Em (nm) |
|
| 分子量 |
|
溶剂 |
|
| 产品详细介绍 |
简要概述
在样品有限或目标分子含量极低的免疫测定中，聚-HRP-抗体结合物被认为具有最高的敏感性。 Buccutite 聚HRP抗体偶联试剂盒旨在快速制备聚HRP偶联抗体。该试剂盒提供了可通过我们专有的Buccutite FOL预激活的poly-HRP探针。用Buccutite MTA（试剂盒中提供）激活靶向抗体，以得到MTA激活的抗体。 MTA激活的抗体与FOL激活的多HRP的简单混合即可得到所需的多HRP标记的抗体偶联物。该反应在极温和的条件下进行而无需催化剂。用Buccutite Poly-HRP抗体偶联试剂盒制备的poly-HRP抗体偶联物与ELISA，western blotting，免疫组化（IHC）分析中使用的发色，荧光和化学发光HRP底物兼容。它也可以与TSA或我们的Styramide 荧光HRP底物一起使用，以超灵敏地检测低丰度生物靶标。
图示
图1.使用Buccutite Poly-HRP抗体偶联试剂盒（Cat＃5518或Cat＃5519）制备的polyHRP-山羊抗小鼠IgG偶联物生成直接ELISA曲线。将3倍系列稀释的小鼠IgG包被在96孔板上，并使用标准ELISA方法检测100uL GAM IgG-polyHRP缀合物（100ng / ml）。用TMB底物溶液（Cat＃11003）孵育5分钟检测固定的小鼠IgG，并在450 nm处读数。
蓝色：5518或5519试剂盒
红色：GXM IgG PolyHRP（供应商A）
绿色：GXM IgG-HRP（供应商B）
参考文献
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说明书
Buccutite 聚HRP抗体偶联试剂盒.pdf
