Whatman 滤膜 8114-6945 CF4 4INX100M 12IN OD PK1 CF4 4INX100M 12IN OD PK1
日度归档:2024年4月28日
56090 Cyanine7.5 carboxylic acid 50 mg
荧光染料 56090 Cyanine7.5 carboxylic acid 50 mg 荧光染料 Cyanine7.5 羧酸 50 mg 695
A1330 Sulfo-Cyanine3 azide 1 mg
水溶性荧光染料叠氮 A1330 Sulfo-Cyanine3 azide 1 mg 水溶性荧光染料叠氮 Sulfo-Cyanine3 叠氮基 1 mg 110
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MP 112055400 λ噬菌体DNA纯化试剂盒
上海金畔生物科技有限公司提供MP 112055400 λ噬菌体DNA纯化试剂盒
品牌:MP
货号:112055400
中文名:λ噬菌体DNA纯化试剂盒
英文名:λQuick kit
CAS号:
纯度:
包装:25preps
价格:2222
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Whatman 滤膜 29167251 MUP G2 0.2uM PTFE 100/PK + HC – PROMO20
Whatman 滤膜 29167251 MUP G2 0.2uM PTFE 100/PK + HC – PROMO20 MUP G2 0.2uM PTFE 100/PK + HC – PROMO20
Whatman 滤膜 3001-6822 1 CHR 0.49INx130M 1/PK
Whatman 滤膜 3001-6822 1 CHR 0.49INx130M 1/PK 1 CHR 0.49INx130M 1/PK
NISSUI 日本日水培养基 Sabouraud Agar with Chloramphenicol (CPSB)
顺式-3-己烯醛 溶液cis-3-Hexenal solution
顺式-3-己烯醛 溶液cis-3-Hexenal solution
grade | FG |
Halal | |
Kosher | |
contains | 0.10% alpha-tocopherol, synthetic as antioxidant |
concentration | 50% in triacetin |
refractive index | n20/D 1.44 |
bp | 20 °C/0.2 mmHg |
grade FG Halal Kosher
contains 0.10% alpha-tocopherol, synthetic as antioxidant
concentration 50% in triacetin
refractive index n20/D 1.44
bp 20 °C/0.2 mmHg
顺式-3-己烯醛 溶液cis-3-Hexenal solution
上海金畔生物科技有限公司代理sigma产品,咨询 详细产品信息
Pall颇尔滤膜 4634 FILTER BOTT-TOP.45UM STER PK10
Pall颇尔滤膜 4634 FILTER BOTT-TOP.45UM STER PK10 214 1712
C2530 Pyrene azide 2 10 mg
荧光染料 C2530 Pyrene azide 2 10 mg 荧光染料 Pyrene 叠氮基 2 10 mg 310
Whatman 滤膜 10371103 603G GLASTHIMBLE 10x38MM 25/PK
Whatman 滤膜 10371103 603G GLASTHIMBLE 10x38MM 25/PK 603G GLASTHIMBLE 10x38MM 25/PK
细胞周期与细胞凋亡检测试剂盒Cell Cycle and Apoptosis Analysis Ki
细胞周期与细胞凋亡检测试剂盒Cell Cycle and Apoptosis Analysis KiT
碘化丙啶(Propidium,简称PI)是一种双链DNA的荧光染料。碘化丙啶和双链DNA结合后可以产生荧光,并且荧光强度和双链DNA的含量成正比。细胞内的DNA被碘化丙啶染色后,可以用流式细胞仪对细胞进行DNA含量测定,然后根据DNA含量的分布情况,可以进行细胞周期和细胞凋亡分析。
碘化丙啶染色后,假设G0/G1期细胞的荧光强度为1,那么含有双份基因组DNA的G2/M期细胞的荧光强度的理论值为2,正在进行DNA复制的S期细胞的荧光强度为1-2之间。凋亡细胞由于细胞核发生浓缩以及发生DNA片段化(DNA fragmentation)导致部分基因组DNA片断在染色过程中丢失,因此凋亡细胞碘化丙啶染色后呈现明显的弱染,即荧光强度小于1,在流式检测的荧光图上出现所谓的sub-G1峰,即凋亡细胞峰。
细胞发生凋亡时,由于胞浆和染色质浓缩、核碎裂,产生凋亡小体,使细胞的光散射性质发生变化。在细胞凋亡的早期,细胞对前向角光散射的能力显著降低,对侧向光散射的能力增加或没有变化。在细胞凋亡的晚期,前向和侧向光散射的信号均降低。因此可通过流式细胞仪测定细胞光散射的变化观察细胞凋亡情况。
本试剂盒通常应用于培养的贴壁或悬浮细胞的细胞周期与细胞凋亡检测。如果用于组织的细胞周期与细胞凋亡检测,则必须把组织消化成单细胞状态,才可以进行检测。
本试剂盒足够检测50个样品,每个样品的细胞数量可以为10-100万。
细胞周期与细胞凋亡检测试剂盒Cell Cycle and Apoptosis Analysis KiT
包装清单:
产品编号 |
产品名称 |
包装 |
C1052-1 |
染色缓冲液 |
25ml |
C1052-2 |
碘化丙啶染色液(20X) |
1.25ml |
C1052-3 |
RNase A(50X) |
0.5ml |
— |
说明书 |
1份 |
保存条件:
-20℃保存,一年有效。C1052-2碘化丙啶染色液(20X)需避光保存。本试剂盒可4℃保存,一个月内有效。
注意事项:
本试剂盒需使用流式细胞仪进行检测。
需自备PBS和70%乙醇,PBS(C0221A)可以向碧云天订购。
荧光染料均存在淬灭问题,保存和使用过程中请尽量注意避光,以减缓荧光淬灭。
碘化丙啶对人体有刺激性,请注意适当防护。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
T 详细产品信息可和选购
Whatman 滤膜 1541-240 541号滤纸圆型240mm/100
Whatman 滤膜 1541-240 541号滤纸圆型240mm/100 GR 541 24CM 100/PK
Whatman 滤膜 1703-050 无灰吸液小片 500g/1
Whatman 滤膜 1703-050 无灰吸液小片 500g/1 ASHLESS CLIPPINGS 500g/PK
Whatman 滤膜 10535174 GR 470 4in x 312FT 1/PK
Whatman 滤膜 10535174 GR 470 4in x 312FT 1/PK GR 470 4in x 312FT 1/PK
OXOID培养基 CM0559B 假单胞菌琼脂基础 PSEUDOMONAS AGAR BASE 500G
OXOID培养基 CM0559B 假单胞菌琼脂基础 PSEUDOMONAS AGAR BASE 500G 711.3789904
NEBNext
NEBNext Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 5) 收藏
Download:
- isoschizomers |
- compatible ends |
- single letter code
NISSUI 日本日水培养基 05221 溴棕三甲胺琼脂培养基 Cetrimide Agar 非发酵性革兰氏阴性杆菌用 300g
sigmaDL-二硫苏糖醇DL-Dithiothreitol
sigmaDL-二硫苏糖醇DL-Dithiothreitol
CAS number: 3483-12-3
Product Description:
Appearance: White Powder
Molecular formula: C4H10O2S2
Melting Point: 42-43°C
Molecular weight: 154.1 g/mol
Solubility: 0.1 M in H2O, 20°C, complete, colorless
pH: 4.0-6.5 (0.1 M in H2O, 25°C)
This product is designated as BioChemika Ultra for Molecular Biology grade and is suitable for applications in the molecular biology, specially if high purity is required. It has been analyzed for low contaminating impurity and the absence of nucleases.
DTT is synthetic. A method of preparation has been described in J. Chem. Soc. 253 (1949)
DTT is an agent for reducing -S-S- to -SH. Redox potential: -0.33 volts at pH 7. DTT acts as a protective agent for -SH groups of other species in solution. In this reaction the DTT is oxidized to the cyclic disulfide, and thereby ensures the reduction of other disulfides in solution. The disulfide reduction is complete in minutes at pH 8. A specific and sensitive assay for disulfide groups is based on determination of resulting monothiols with DTNB (Fluka 43760).2
Applications:
It is used in buffers for isolation of RNA5 or buffers used for RT-PCR starting with mRNA6,7.
Precautions and Disclaimer:
For Laboratory Use Only. Not for drug, household or other uses.
Preparation Instructions:
The solubility of DTT has been tested by Fluka at 50 mg/ml in water, resulting in a clear colorless solution. It has been described as freely soluble in water, ethanol, acetone, ethylate, chloroform and ether.
Solutions oxidize relatively slowly in air.3 It is recommended to make solutions fresh daily. Recorded half-life (hours) of DTT solutions at various pH and temperatures are as follows:4
Conditions (all in 0.1 M KPO4 buffer)
Half life (Hours)
pH 6.5 at 20°C
40
pH 7.5 at 20°C
10
pH 8.5 at 20°C
1.4
pH 8.5 at 0°C
11
pH 8.5 at 40°C
0.2
pH 8.5 at 20°C +0.1 mM Cu2+
0.6
pH 8.5 at 20°C +0.1 mM EDTA
4
Storage/Stability:
Storage Temperature: 4°C
When stored as indicated, this product is stable for years.
上海金畔生物科技有限公司代理sigma产品,咨询sigmaDL-二硫苏糖醇DL-Dithiothreitol 详细产品信息