详细描述: Transforming growth factor b (TGFβ) receptor associated binding protein (TRAP-1) participates in the regulation of the TGFβ signaling pathway. TGFβ is a secreted ligand that induces transcription of various targeted genes involved in cell proliferation, d
保存条件: -20℃保存,至少一年有效。 注意事项: SDS-PAGE蛋白上样缓冲液(5X)中含少量DTT和巯基乙醇,有轻微刺激性气味。 (5X)必须完全溶解后再使用。 为了您的安全和健康,请穿实验服并戴一次性手套操作。 使用说明: 1. 在室温或不超过37℃的水浴中溶解SDS-PAGE上样缓冲液(5X)。水浴溶解后立即室温存放,尽量避免长时间置于水浴中。 2. 按照每4微升蛋白样品加入1微升蛋白上样缓冲液(5X)的比例,混合蛋白样品和蛋白上样缓冲液(5X)。 3. 100℃或沸水浴加热3-5分钟,以充分变性蛋白。 4. 冷却到室温后,直接上样到SDS-PAGE胶加样孔内即可。 5. 通常电泳至蓝色染料到达胶的底端处附近即可停止电泳。 使用本产品的文献: 1. Cai H, Yin D, Zhang L, Yang X, Xu X, Liu W, Zheng X, Zhang H, Wang J, Xu Y, Cheng D, Zheng M, Han Y, Wu M, Wang Y. Preparation and biological evaluation of 2-amino-6-[18F]fluoro-9-(4-hydroxy-3-hydroxy-methylbutyl) purine (6-[18F]FPCV) as a novel PET probe for imaging HSV1-tk reporter gene expression. Nucl Med Biol. 2007 Aug;34(6):717-25. 2. Yuan Q, Li PD, Li BH, Yang XZ, Xu SB, Liu XH, Zhou FX, Zhang WJ. Differential IL-4/Stat6 activities correlate with differential expression of regulatory genes SOCS-1, SHP-1, and PP2A in colon cancer cells. J Cancer Res Clin Oncol. 2008 Jun 7. 3. Li H, Zhang L, Huang Q. Differential expression of mitogen-activated protein kinase signaling pathway in the hippocampus of rats exposed to chronic unpredictable stress. Behav Brain Res. 2009 Dec 14;205(1):32-7. Epub 2009 Jul 1. 4. Cao BY, Yang YP, Luo WF, Mao CJ, Han R, Sun X, Cheng J, Liu CF. Paeoniflorin, a potent natural compound, protects PC12 cells from MPP+ and acidic damage via autophagic pathway. J Ethnopharmacol. 2010;131(1):122-9. Epub 2010 Jun 15.