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品牌：Cosmo Bio
CAS No.： 储存条件：室温 纯度：– |
| 产品编号
(生产商编号) |
等级 | 规格 | 运输包装 | 零售价(RMB) | 库存情况 | 参考值 |
|
ISO-AP-1826-100 |
– | 1 Set | – | 咨询 | – | – |
* 干冰运输、大包装及大批量的产品需酌情添加运输费用
* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。
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品牌：Tokken
CAS No.： 储存条件：室温 纯度：– |
| 产品编号
(生产商编号) |
等级 | 规格 | 运输包装 | 零售价(RMB) | 库存情况 | 参考值 |
|
SK-200X |
– | 1 kit | – | 咨询 | – | – |
* 干冰运输、大包装及大批量的产品需酌情添加运输费用
* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。
Hampton Research蛋白结晶试剂盒
Products > Optimize Reagents > Optimize – Salts > Magnesium sulfate hydrate
Magnesium sulfate hydrate
Synonyms: Magnesium sulphate hydrate
MgSO4 • aqueous
Mr 120.37 • aqueous
CAS Number [22189-08-8]
EC Number 231-298-2
MDL Number MFCD00011110
PubChem Substance ID 24882418
Purity ≥ 99.0%
Measured pH Range: 7.8 – 8.3 at 25°C
Measured Conductivity Range: 57.9 – 59.1 mS/cm at 25°C
Measured Refractive Index Range: 1.36702 – 1.37174 at 20°C
Ca ≤0.02%
Cd ≤0.005%
Cl ≤0.01%
Co ≤0.005%
Cu ≤0.005%
Fe ≤0.005%
K ≤0.05%
Na ≤0.01%
Ni ≤0.005%
Pb ≤0.005%
Zn ≤0.005%
Click to Zoom In
HR2-633
2.5 M Magnesium sulfate hydrate
200 mL
$103.00
cart quote
HR2-633 Magnesium sulfate hydrate SDS 1. Rudikoff, S; Potter, M; Segal, DM; Padlan, EA; Davies, DR; Proc Natl Acad Sci U S A 69, 3689- 3692, 1972.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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lumiprobe荧光染料官网代理商 水溶性荧光染料代理商 脂溶性荧光染料代理商
| Cat. # | Quantity | Price | Lead time | Buy this product | ||
|---|---|---|---|---|---|---|
| 15420 | 1 mg | 110.00$
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| 25420 | 5 mg | 210.00$
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| 45420 | 25 mg | 410.00$
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| 55420 | 50 mg | 695.00$
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| 65420 | 100 mg | 1190.00$
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BDP 630/650 is a borondipyrromethene fluorophore that has a high molar extinction coefficient, excellent quantum yield, and a relatively long lifetime of the excited state. Due to it, this fluorophore is useful for fluorescence polarization assays that allow to detect binding between molecules.
This is an amine reactive NHS ester. It contains an aminohexanoyl linker between the fluorophore and the reactive group.
Download in
ChemDraw format
BDP 630/650 maleimide is a thiol reactive, borondipyrromethene based dye tuned to match the far red channel of Cyanine5 fluorophore.
Hampton Research蛋白结晶试剂盒
Products > Custom Shop Crystallization Reagents > StockOptions MES > Individual StockOptions MES Reagents
HR2-943-**
** Refers to the reagent number in the StockOptions MES Buffer kit.
For example, reagent number 1 = HR2-943-01.
MES monohydrate
Synonyms: MES, 2-(N-morpholino)ethanesulfonic acid
or 4-morpholineethanesulfonic acid monohydrate
C6H13NO4S · H2O
Mr 213.25
CAS Number [145224-94-8]
EC Number 224-632-3
Beilstein Registry Number 6350956
RTECS QE3479500
MDL Number MFCD00149409
PubChem Substance ID 24885996
Purity >99.0%
pKa 6.1 at 25°C
Solutions are stable at 2-8°C for months. Autoclaving is not recommended for any sulfonic acid buffer. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown. When MES solutions are stored at room temperature, they can turn yellow within six months.
Useful pH range of MES 5.2 – 7.1
MES buffers formulated in Type 1+ ultrapure water, 18.2 megohm resistivity at 25°C, < 5 ppb Total Organic Carbon, <1 Bacteria (CFU/ml), <0.03 Endotoxin (EU/ml). The pH is adjusted using Sodium hydroxide (NaOH). Buffers are sterile filtered using 0.22 micron filters under sterile conditions
Ca < 0.005%
Cd < 0.0005%
Cl < 0.005%
Co < 0.0005%
Cr < 0.0005%
Cu < 0.0005%
Fe < 0.0005%
K < 0.005%
Mg < 0.0005%
Mn < 0.0005%
Na < 0.005%
Ni < 0.0005%
Pb < 0.0005%
SO4 < 0.005%
Zn < 0.0005%
Click to Zoom In
HR2-943-01
StockOptions MES 01 185ml
1.0 M MES monohydrate pH 5.2
$180.00
cart quote
HR2-943-02
StockOptions MES 02 185ml
1.0 M MES monohydrate pH 5.3
$180.00
cart quote
HR2-943-03
StockOptions MES 03 185ml
1.0 M MES monohydrate pH 5.4
$180.00
cart quote
HR2-943-04
StockOptions MES 04 185ml
1.0 M MES monohydrate pH 5.5
$180.00
cart quote
HR2-943-05
StockOptions MES 05 185ml
1.0 M MES monohydrate pH 5.6
$180.00
cart quote
HR2-943-06
StockOptions MES 06 185ml
1.0 M MES monohydrate pH 5.7
$180.00
cart quote
HR2-943-07
StockOptions MES 07 185ml
1.0 M MES monohydrate pH 5.8
$180.00
cart quote
HR2-943-08
StockOptions MES 08 185ml
1.0 M MES monohydrate pH 5.9
$180.00
cart quote
HR2-943-09
StockOptions MES 09 185ml
1.0 M MES monohydrate pH 6.0
$180.00
cart quote
HR2-943-10
StockOptions MES 10 185ml
1.0 M MES monohydrate pH 6.1
$180.00
cart quote
HR2-943-11
StockOptions MES 11 185ml
1.0 M MES monohydrate pH 6.2
$180.00
cart quote
HR2-943-12
StockOptions MES 12 185ml
1.0 M MES monohydrate pH 6.3
$180.00
cart quote
HR2-943-13
StockOptions MES 13 185ml
1.0 M MES monohydrate pH 6.4
$180.00
cart quote
HR2-943-14
StockOptions MES 14 185ml
1.0 M MES monohydrate pH 6.5
$180.00
cart quote
HR2-943-15
StockOptions MES 15 185ml
1.0 M MES monohydrate pH 6.6
$180.00
cart quote
HR2-943-16
StockOptions MES 16 185ml
1.0 M MES monohydrate pH 6.7
$180.00
cart quote
HR2-943-17
StockOptions MES 17 185ml
1.0 M MES monohydrate pH 6.8
$180.00
cart quote
HR2-943-18
StockOptions MES 18 185ml
1.0 M MES monohydrate pH 6.9
$180.00
cart quote
HR2-943-19
StockOptions MES 19 185ml
1.0 M MES monohydrate pH 7.0
$180.00
cart quote
HR2-943-20
StockOptions MES 20 185ml
1.0 M MES monohydrate pH 7.1
$180.00
cart quote
HR2-943-** Individual StockOptions MES Reagents SDS 1. Microscale Fluorescent Thermal Stability Assay for Membrane Proteins Structure, Volume 16, Issue 3, 11 March 2008, Pages 351-359 Alexander I. Alexandrov, Mauro Mileni, Ellen Y.T. Chien, Michael A. Hanson, Raymond C. Stevens
2. Characterization, crystallization and preliminary crystallographic analysis of human recombinant cyclooxygenase-2. Marco, SD; Priestle, JP; Grutter, MG; Wennogle, LP; Boyar, W. Acta Crystallogr D 53, 224- 226, 1997.
3. Increasing the size of microcrystals by fine sampling of pH limits. A. McPherson. J. Appl. Cryst. (1995). 28, 362-365.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
| 货号 | 13813 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 200 Tests | 价格 | 3840 |
| Ex (nm) | 575 | Em (nm) | |
| 分子量 | 溶剂 | ||
| 产品详细介绍 | |||
简要概述
Amplite 比色法L-乳酸脱氢酶（LDH）检测试剂盒是美国AAT Bioquest生产的用于检测乳酸的试剂盒，乳酸脱氢酶（LDH ）是一种可催化丙酮酸和乳酸的相互转换氧化还原酶。 LDH存在于各种各样的生物体，包括动物和植物的细胞质中。细胞组织的损伤或红细胞溶血后释放的LDH进入血液。因为LDH是相当稳定的酶，它已被广泛地用于评价和组织细胞毒性的存在。乳酸脱氢酶的定量具有广阔的应用范围。 LDH可在某些病理条件下提高，如癌症。这种Amplite 乳酸脱氢酶测定试剂盒提供了用于生物样品中L-乳酸脱氢酶（ L- LDH）基于荧光检测的方法，如血清，血浆，尿液，以及细胞培养物的样品。吸收信号可以由吸收酶标仪在〜 575nm处读取。有了这个Amplite L-乳酸脱氢酶测定试剂盒，我们能够探测到低至3 mU/mL的L-乳酸脱氢酶在100 μL的反应体积。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Amplite 比色法L-乳酸脱氢酶（LDH）检测试剂盒。
适用仪器
| 光吸收酶标仪 | |
| 吸收： | 575/605nm |
| 推荐孔板： | 透明底板 |
产品说明书
96孔板测定示例
概述
制备D-乳酸测定混合物（50μL）
加入D-乳酸标准品或测试样品（50μL）
在室温下孵育30分钟~2小时
监测吸光度比率在A575nm / A605nm处增加
注意：在开始实验之前，在室温下解冻每个试剂盒组分的一个小瓶。
操作方法
1.准备NAD储备溶液（100X）：
向NAD小瓶（组分C）中加入100μLH2O，制成100X NAD储备溶液。
2.准备D-乳酸储备液：
将200μLH2 O或1×PBS缓冲液加入到D-乳酸盐标准品（组分D）的小瓶中以制备100mM D-乳酸盐标准溶液。
注意：未使用的D-lacate标准储备溶液应分成单次使用的等分试样并储存在-20℃。
3.准备D-乳酸标准品（0至1 mM）的连续稀释液：
3.1将10μLD-乳酸储备溶液（来自步骤2）加入990L 1x PBS缓冲液中以产生1mM D-乳酸盐标准溶液。
注意：稀释的D-乳酸标准溶液不稳定，应在4小时内使用。
3.2取200μL的1mM D-乳酸标准溶液进行1：3连续稀释，得到300,100,30,10,3,1和0μM的D-乳酸标准品系列稀释液。
3.3如说明书中的表1和2中所述，将含有D-乳酸盐标准品和含D-乳酸盐的测试样品的系列稀释液加入白色透明底96孔微量培养板中。
4.准备D-乳酸测定混合物：
4.1将5 mL测定缓冲液（组分B）加入一瓶酶混合物（组分A）中。
4.2将50μLNAD储备液（100X，来自步骤1）加入组分A（来自步骤4.1）的瓶中，并且混合均匀。
注意：这种D-乳酸测定混合物足以用于一个96孔板。 未使用的D-乳酸测定混合物应分成单次使用的等分试样并储存在-20℃。
5.运行D-乳酸测定：
5.1将50μLD-乳酸测定混合物（来自步骤4.2）添加到D-乳酸标准品，空白对照和测试样品（参见步骤3.3）的每个孔中，以使总D-乳酸测定体积为100μL/孔。
注意：对于384孔板，每孔加入25μL样品和25μLD-乳酸测定混合物。
5.2在室温下孵育反应30分钟至2小时，避光。
5.3用A575nm / A605nm的吸光度读板仪检测吸光度比率的增加。
参考文献
Dendrobium huoshanense polysaccharide regionally regulates intestinal mucosal barriers function and intestinal microbiota in mice
Authors: Song-Zi Xie, Bing Liu, Hui-Yu Ye, Qiang-Ming Li, Li-Hua Pan, Xue-Qiang Zha, Jian Liu, Jun Duan, Jian-Ping Luo
Journal: Carbohydrate Polymers (2018)
Exploring the Probiotic and Compound Feed Fermentative Applications of Lactobacillus plantarum SK1305 Isolated from Korean Green Chili Pickled Pepper
Authors: Kai-Min Niu, Damini Kothari, Sang-Buem Cho, Sung-Gu Han, In-Geun Song, Sam-Churl Kim, Soo-Ki Kim
Journal: Probiotics and Antimicrobial Proteins (2018): 1–12
Arenobufagin inhibits prostate cancer epithelial-mesenchymal transition and metastasis by down-regulating β-catenin
Authors: Liping Chen, Weiqian Mai, Minfeng Chen, Jianyang Hu, Zhenjian Zhuo, Xueping Lei, Lijuan Deng, Junshan Liu, Nan Yao, Maohua Huang
Journal: Pharmacological Research (2017)
Fibroblast Activation Protein Alpha-Activated Tripeptide Bufadienolide Anti-tumor Prodrug with Reduced Cardiotoxicity
Authors: Li-Juan Deng, Long-Hai Wang, Cheng-Kang Peng, Yi-Bin Li, Mao-Hua Huang, Min-Feng Chen, Xue-Ping Lei, Ming Qi, Yun Cen, Wen-Cai Ye
Journal: Journal of Medicinal Chemistry (2017)
Neuronal Culture Microenvironments Determine Preferences in Bioenergetic Pathway Use
Authors: Juliane Sünwoldt, Bert Bosche, Andreas Meisel, Philipp Mergenthaler
Journal: Frontiers in Molecular Neuroscience (2017): 305
The use of KnockOut serum replacement (KSR) in three dimentional rat testicular cells co-culture model: An improved male reproductive toxicity testing system
Authors: Xiaofang Zhang, Lei Wang, Xiaodong Zhang, Lijun Ren, Wenjing Shi, Yijun Tian, Jiangbo Zhu, Tianbao Zhang
Journal: Food and Chemical Toxicology (2017)
High glucose and interleukin 1β-induced apoptosis in human umbilical vein endothelial cells involves in down-regulation of monocarboxylate transporter 4
Authors: Dong Wang, Qingjie Wang, Gaoliang Yan, Yong Qiao, Ling Sun, Boqian Zhu, Chengchun Tang, Yuchun Gu
Journal: Biochemical and biophysical research communications (2015): 607–614
Intracellular ATP decrease mediates NLRP3 inflammasome activation upon nigericin and crystal stimulation
Authors: Johji Nomura, Alexander So, Mizuho Tamura, Nathalie Busso
Journal: The Journal of Immunology (2015): 5718–5724
相关产品
| 产品名称 | 货号 |
| Amplite 比色法D-乳酸脱氢酶（LDH）检测试剂盒 | Cat#13809 |
| Amplite 荧光法L-乳酸脱氢酶（LDH）检测试剂盒 | Cat#13812 |
| Amplite 比色法L-乳酸检测试剂盒 | Cat#13815 |
上海金畔生物科技有限公司代理Takara酶试剂盒全线产品，欢迎访问官网了解更多产品信息。
| 热启动PCR用TaqStart 抗体 | ||||||
| 品牌 | Code No. | 产品名称 | 包装量 | 价格（元） | 说明书 | 数量 |
| Clontech | 639250 | TaqStart® Antibody | 200 Rxns | ￥2,722 |  |
  |
| Clontech | 639251 | TaqStart® Antibody | 500 Rxns | ￥5,095 | |
|
| TaqStart Antibody是抗体介导的热启动技术，增强PCR的特异性和灵敏度。 该抗体在热循环开始前抑制聚合酶活性，防止非特异性扩增和引物二聚体形成。 当反应温度升高时，抗体快速失活并进行PCR。 |
| 产品特点 |
| · 可在室温配置反应液 · 更高的特异性，背景降低。无需摸索反应条件 · 热启动PCR技术性价比高的方法 · 抗体可与任何全长Taq聚合酶一起使用 · 可提供订制大包装 |
产品详情请点击： |
页面更新：2021-05-11 15:54:48
truCOLLECT血液样本提取试剂盒(10)
truCOLLECT™-RUO DNA Extraction & Purification Kit – Column Purification (10)
| 货号 | 产品名称 | 品牌 | 购买 |
|---|
| 货号 | 名称 | 单位 | 购买 |
|---|---|---|---|
| 520236 | truCOLLECT血液样本提取试剂盒(10) | 10次/ kit | 咨询客服 |
Ret (E1N8X) XP® 兔单克隆抗体
Ret (E1N8X) XP® Rabbit mAb
| 货号 | 产品名称 | 品牌 | 购买 |
|---|
| 货号 | 名称 | 单位 | 购买 |
|---|---|---|---|
| 14556T | Ret (E1N8X) XP® 兔单克隆抗体 | 20 µl | 咨询客服 |
| 14556S | Ret (E1N8X) XP® 兔单克隆抗体 | 100µl | 咨询客服 |
Hampton Research蛋白结晶试剂盒
Products > Optimize Reagents > Optimize – Salts > DL-Malic acid pH 7.0
DL-Malic acid
Synonyms: (±)-2-Hydroxysuccinic acid or DL-Hydroxybutanedioic acid
HO2CCH2CH(OH)CO2H
C4H6O5
Mr 134.09
CAS Number [6915-15-7]
EC Number 230-022-8
Beilstein Registry Number 1723539
RTECS ON7175000
MDL Number MFCD00064212
PubChem Substance ID 24901221
Purity ≥ 99.0%
Appearance (Solution): Clear, yellow to light brown
HR2-761 is titrated to pH 7.0 using NaOH
Measured Conductivity Range: 48.0 – 54.4 mS/cm at 25°C
Measured Refractive Index Range: 1.40572 – 1.40617 at 20°C
pKa1 3.4
pKa2 5.1
As ≤0.0003%
Cd ≤0.0001%
Hg ≤0.0001%
Pb ≤0.001%
Click to Zoom In
HR2-761
3.0 M DL-Malic acid pH 7.0
200 mL
$77.00
cart quote
HR2-761 DL-Malic acid pH 7.0 SDS 1. A comparison of salts for the crystallization of macromolecules. Alexander McPherson. Protein Science (2001), 10:418–422.
2. Novel homozygous p.E64D mutation in DJ1 in early onset Parkinson disease (PARK7). Olaf Riess et al. Human Mutation, Volume 24, Issue 4, Pages 321 – 329, 2004.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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品牌：Funakoshi
CAS No.： 储存条件：参考产品说明书 纯度：– |
| 产品编号
(生产商编号) |
等级 | 规格 | 运输包装 | 零售价(RMB) | 库存情况 | 参考值 |
|
893003 |
– | 2 pieces | – | 咨询 | – | – |
* 干冰运输、大包装及大批量的产品需酌情添加运输费用
* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。
|
品牌：Sumitomo Bakelite
CAS No.： 储存条件：室温 纯度：– |
| 产品编号
(生产商编号) |
等级 | 规格 | 运输包装 | 零售价(RMB) | 库存情况 | 参考值 |
|
MS-2325RS |
– | 30个 | – | 咨询 | – | – |
* 干冰运输、大包装及大批量的产品需酌情添加运输费用
* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。
NEBuffer 1.1: 10%
NEBuffer 2.1: 100%
NEBuffer 3.1: 10%
CutSmart Buffer: 100%
CutSmart、重组酶、省时酶。
CutSmart 缓冲液，37℃。
10,000 units/ml。
对哺乳动物基因组 DNA CpG 甲基化敏感。
lumiprobe荧光染料官网代理商 水溶性荧光染料代理商 脂溶性荧光染料代理商
| Cat. # | Quantity | Price | Lead time | Buy this product | ||
|---|---|---|---|---|---|---|
| 1E420 | 1 mg | 110.00$
|
||||
| 2E420 | 5 mg | 210.00$
|
||||
| 4E420 | 25 mg | 410.00$
|
||||
| 5E420 | 50 mg | 695.00$
|
||||
| 6E420 | 100 mg | 1190.00$
|
||||
BDP 650/665 is a borondipyrromethene dye with far red excitation and emission. The dye has a good molar extinction coefficient and emission quantum yield.
The NHS ester can be conjugated with amine groups of proteins, peptides, and other molecules. The molecule contains an additional aminohexanoic acid linker.
Download in
ChemDraw format
BDP 576/589 NHS ester (BODIPY™ 576/589 analog) is a bright hydrophobic dye with emission in the orange spectrum range. It can be used in fluorescence anisotropy assays.
Hampton Research蛋白结晶试剂盒
Products > Custom Shop Crystallization Reagents > StockOptions MOPS > Individual StockOptions MOPS Reagents
HR2-252-**
** = Refers to the reagent number in the kit.
For example, reagent number 1 = HR2-252-01
Synonyms: 3-(N-Morpholino)propanesulfonic acid, 4-Morpholinepropanesulfonic acid
C7H15NO4S
Mr 209.28
CAS Number [1132-61-2]
EC Number 214-478-5
RTECS QE9104530
Beilstein Registry Number 1106776
MDL Number MFCD00006183
PubChem Substance ID 24896626
Purity ≥99.5%
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HR2-252-01
StockOptions MOPS 01 185ml
1.0 M MOPS pH 6.5
$171.00
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HR2-252-02
StockOptions MOPS 02 185ml
1.0 M MOPS pH 6.6
$171.00
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HR2-252-03
StockOptions MOPS 03 185ml
1.0 M MOPS pH 6.7
$171.00
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HR2-252-04
StockOptions MOPS 04 185ml
1.0 M MOPS pH 6.8
$171.00
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HR2-252-05
StockOptions MOPS 05 185ml
1.0 M MOPS pH 6.9
$171.00
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HR2-252-06
StockOptions MOPS 06 185ml
1.0 M MOPS pH 7.0
$171.00
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HR2-252-07
StockOptions MOPS 07 185ml
1.0 M MOPS pH 7.1
$171.00
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HR2-252-08
StockOptions MOPS 08 185ml
1.0 M MOPS pH 7.2
$171.00
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HR2-252-09
StockOptions MOPS 09 185ml
1.0 M MOPS pH 7.3
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HR2-252-10
StockOptions MOPS 10 185ml
1.0 M MOPS pH 7.4
$171.00
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HR2-252-11
StockOptions MOPS 11 185ml
1.0 M MOPS pH 7.5
$171.00
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HR2-252-12
StockOptions MOPS 12 185ml
1.0 M MOPS pH 7.6
$171.00
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HR2-252-13
StockOptions MOPS 13 185ml
1.0 M MOPS pH 7.7
$171.00
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HR2-252-14
StockOptions MOPS 14 185ml
1.0 M MOPS pH 7.8
$171.00
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HR2-252-15
StockOptions MOPS 15 185ml
1.0 M MOPS pH 7.9
$171.00
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HR2-252-** Individual StockOptions MOPS Reagents SDS 
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
| 货号 | 13814 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 200 Tests | 价格 | 3840 |
| Ex (nm) | 571 | Em (nm) | 585 |
| 分子量 | 溶剂 | ||
| 产品详细介绍 | |||
简要概述
Amplite 荧光法L-乳酸检测试剂盒是美国AAT Bioquest生产的用于检测乳酸的试剂盒，乳酸具有两种光学异构体： L-乳酸和D-乳酸。乳酸是不断从丙酮酸通过酶乳酸脱氢酶（LDH ）的代谢和运动的过程产生的。研究细胞和动物生理学的时候是非常有用的。 D-乳酸是不被哺乳动物代谢，其从体内消除主要依赖于肾排泄。 D-和L -乳酸被发现在许多发酵乳产品，如酸奶和奶酪，以及在泡菜和腌制肉类和鱼类。D-和L -乳酸（由细菌产生的）是食品，如鸡蛋，牛奶，果汁和酒的品质指标。异常高浓度的D-乳酸在血液中通常是细菌过度生长在胃肠道中的反映。 AAT Bioquest的Amplite 乳酸检测试剂盒（目录号13814和13815对L -乳酸检测，和Cat ＃ 13810和13811的D-乳酸检测），用于检测L-乳酸或D -乳酸同时提供荧光和吸光度为基础的方法生物样品如血清，血浆，以及细胞培养物的样品。该信号可通过荧光酶标仪在EX/ EM = 540 nm/590 nm处读取。与此荧光Amplite D-乳酸检测试剂盒，我们能够检测少至1.4uM的D-乳酸在100微升反应体积。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Amplite 荧光法L-乳酸检测试剂盒。
适用仪器
| 荧光酶标仪 | |
| 激发： | 540nm |
| 发射： | 590nm |
| cutoff： | 570nm |
| 推荐孔板： | 黑色孔板 |
产品说明书
96孔板测定示例
概述
制备D-乳酸测定混合物（50μL）
加入D-乳酸标准品或测试样品（50μL）
在室温下孵育30分钟~2小时
监测吸光度比率在A575nm / A605nm处增加
注意：在开始实验之前，在室温下解冻每个试剂盒组分的一个小瓶。
操作方法
1.准备NAD储备溶液（100X）：
向NAD小瓶（组分C）中加入100μLH2O，制成100X NAD储备溶液。
2.准备D-乳酸储备液：
将200μLH2 O或1×PBS缓冲液加入到D-乳酸盐标准品（组分D）的小瓶中以制备100mM D-乳酸盐标准溶液。
注意：未使用的D-lacate标准储备溶液应分成单次使用的等分试样并储存在-20℃。
3.准备D-乳酸标准品（0至1 mM）的连续稀释液：
3.1将10μLD-乳酸储备溶液（来自步骤2）加入990L 1x PBS缓冲液中以产生1mM D-乳酸盐标准溶液。
注意：稀释的D-乳酸标准溶液不稳定，应在4小时内使用。
3.2取200μL的1mM D-乳酸标准溶液进行1：3连续稀释，得到300,100,30,10,3,1和0μM的D-乳酸标准品系列稀释液。
3.3如说明书中的表1和2中所述，将含有D-乳酸盐标准品和含D-乳酸盐的测试样品的系列稀释液加入白色透明底96孔微量培养板中。
4.准备D-乳酸测定混合物：
4.1将5 mL测定缓冲液（组分B）加入一瓶酶混合物（组分A）中。
4.2将50μLNAD储备液（100X，来自步骤1）加入组分A（来自步骤4.1）的瓶中，并且混合均匀。
注意：这种D-乳酸测定混合物足以用于一个96孔板。 未使用的D-乳酸测定混合物应分成单次使用的等分试样并储存在-20℃。
5.运行D-乳酸测定：
5.1将50μLD-乳酸测定混合物（来自步骤4.2）添加到D-乳酸标准品，空白对照和测试样品（参见步骤3.3）的每个孔中，以使总D-乳酸测定体积为100μL/孔。
注意：对于384孔板，每孔加入25μL样品和25μLD-乳酸测定混合物。
5.2在室温下孵育反应30分钟至2小时，避光。
5.3用A575nm / A605nm的吸光度读板仪检测吸光度比率的增加。
参考文献
Dendrobium huoshanense polysaccharide regionally regulates intestinal mucosal barriers function and intestinal microbiota in mice
Authors: Song-Zi Xie, Bing Liu, Hui-Yu Ye, Qiang-Ming Li, Li-Hua Pan, Xue-Qiang Zha, Jian Liu, Jun Duan, Jian-Ping Luo
Journal: Carbohydrate Polymers (2018)
Exploring the Probiotic and Compound Feed Fermentative Applications of Lactobacillus plantarum SK1305 Isolated from Korean Green Chili Pickled Pepper
Authors: Kai-Min Niu, Damini Kothari, Sang-Buem Cho, Sung-Gu Han, In-Geun Song, Sam-Churl Kim, Soo-Ki Kim
Journal: Probiotics and Antimicrobial Proteins (2018): 1–12
Arenobufagin inhibits prostate cancer epithelial-mesenchymal transition and metastasis by down-regulating β-catenin
Authors: Liping Chen, Weiqian Mai, Minfeng Chen, Jianyang Hu, Zhenjian Zhuo, Xueping Lei, Lijuan Deng, Junshan Liu, Nan Yao, Maohua Huang
Journal: Pharmacological Research (2017)
Fibroblast Activation Protein Alpha-Activated Tripeptide Bufadienolide Anti-tumor Prodrug with Reduced Cardiotoxicity
Authors: Li-Juan Deng, Long-Hai Wang, Cheng-Kang Peng, Yi-Bin Li, Mao-Hua Huang, Min-Feng Chen, Xue-Ping Lei, Ming Qi, Yun Cen, Wen-Cai Ye
Journal: Journal of Medicinal Chemistry (2017)
Neuronal Culture Microenvironments Determine Preferences in Bioenergetic Pathway Use
Authors: Juliane Sünwoldt, Bert Bosche, Andreas Meisel, Philipp Mergenthaler
Journal: Frontiers in Molecular Neuroscience (2017): 305
The use of KnockOut serum replacement (KSR) in three dimentional rat testicular cells co-culture model: An improved male reproductive toxicity testing system
Authors: Xiaofang Zhang, Lei Wang, Xiaodong Zhang, Lijun Ren, Wenjing Shi, Yijun Tian, Jiangbo Zhu, Tianbao Zhang
Journal: Food and Chemical Toxicology (2017)
High glucose and interleukin 1β-induced apoptosis in human umbilical vein endothelial cells involves in down-regulation of monocarboxylate transporter 4
Authors: Dong Wang, Qingjie Wang, Gaoliang Yan, Yong Qiao, Ling Sun, Boqian Zhu, Chengchun Tang, Yuchun Gu
Journal: Biochemical and biophysical research communications (2015): 607–614
Intracellular ATP decrease mediates NLRP3 inflammasome activation upon nigericin and crystal stimulation
Authors: Johji Nomura, Alexander So, Mizuho Tamura, Nathalie Busso
Journal: The Journal of Immunology (2015): 5718–5724
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| 产品名称 | 货号 |
| Amplite 荧光法D-乳酸检测试剂盒 | Cat#13810 |
| Amplite 荧光法L-丙氨酸检测试剂盒 | Cat#13825 |
| Amplite 比色法L-乳酸检测试剂盒 | Cat#13815 |
Synaptotagmin-1 (D33B7) 兔单克隆抗体
Synaptotagmin-1 (D33B7) Rabbit mAb
| 货号 | 产品名称 | 品牌 | 购买 |
|---|
| 货号 | 名称 | 单位 | 购买 |
|---|---|---|---|
| 14558S | Synaptotagmin-1 (D33B7) 兔单克隆抗体 | 100µl | 咨询客服 |
|
品牌：FUJIFILM Wako
CAS No.：63231-67-4 储存条件：室温 纯度：– |
| 产品编号
(生产商编号) |
等级 | 规格 | 运输包装 | 零售价(RMB) | 库存情况 | 参考值 |
|
231-02717 |
for Column Chromatography | 10kg | – | 6,420.00 | – | – |
* 干冰运输、大包装及大批量的产品需酌情添加运输费用
* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。
Hampton Research蛋白结晶试剂盒
Products > Optimize Reagents > Optimize – Salts > Nickel(II) chloride hexahydrate
Nickel(II) chloride hexahydrate
Synonyms: Nickel chloride
NiCl2 • 6H2O
Mr 237.69
CAS Number [7791-20-0]
EC Number 231-743-0
Merck 14,6505
RTECS QR6480000
UN Number 3288 6.1/PG 3
MDL Number MFCD00149809
PubChem Substance ID 24858970
Purity ≥ 98.0%
Measured pH Range: 2.3 – 3.8 at 25°C
Measured Conductivity Range: 102.6 – 108.7 mS/cm at 25°C
Measured Refractive Index Range: 1.43676 – 1.43997 at 20°C
Ca ≤0.005%
Cd ≤0.001%
Co ≤0.01%
Cu ≤0.001%
Fe ≤0.002%
Mg ≤0.001%
Na ≤0.005%
Pb ≤0.001%
SO4 ≤0.005%
Zn ≤0.001%
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HR2-687
4.0 M Nickel(II) chloride hexahydrate
100 mL
$70.00
Temporarily Unavailable
More Available Soon!
HR2-687 Nickel(II) chloride hexahydrate SDS 1. Purification and crystallization of Pseudomonas aeruginosa chloramphenicol acetyltransferase. Tian, Y; Beaman, TW; Roderick, SL. Proteins 28, 298- 300, 1997.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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