mWM (modified Whitten’s Medium) mWM小鼠体外培养基 品牌:Cosmo Bio


品牌:Cosmo Bio
CAS No.:
储存条件:+4℃ DNF
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

CSR-R-B081

10 x 5 ml 咨询


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Wakopak Wakosil5C18-200T 4.6*150mm 硅胶柱5C18-200T 品牌:FUJIFILM Wako


Wakopak Wakosil5C18-200T 4.6*150mm

硅胶柱5C18-200T

品牌:FUJIFILM Wako
CAS No.:
储存条件:室温
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

234-55753

1 column(W) 咨询


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


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8 联排管oneTUBE-10 AFA (12) 8 oneTUBE-10 AFA Strip (12)

8 联排管oneTUBE-10 AFA (12)

8 oneTUBE-10 AFA Strip (12)

详细描述:
8 联排管oneTUBE-10 AFA,适用于ME220 and LE220-plus。 需要Rack 500608和Waveguide 500526 用于LE220-plus 需要Rack 500609用于ME220. 可配8联排铝箔封膜520108或strip cap 8联排盖子500613 oneTUBE系列是由特殊聚合物材质合成耗材,用于实现NGS 打断建库一体化流程,在完成DNA片段化后,末端修复、接头连接以及PCR反应均可在oneTUBE一管中进行,整个文库构建过程中无需再进行样本转移, 避免了移液换液过程中的损失和污染。oneTUBE的管壁经过低吸附处理,更少可以进行10μl样本(或pg级样本)的处理,特别适合微量样本的自动化建库处理,为客户们解决文库起始量低的困难。 oneTUBE系列耗材具有以下特点: 1.便于样本追踪管理:支持条码扫描和LIMS数据自动采集; 2.自动化整合,简化工作流程:与其他实验仪器适配,流程化高通量工作 3.消除实验转移步骤,管壁低吸附处理,提高样本回收率; 4.使用AFA技术,获得精准、可重复结果; 5.DNA打断体积从10ul到50ul,浓度可达100ng/μl; 6.支持加盖和贴箔封口; 7.下游容量可达200ul,适用于各种建库体系。

适用平台:Covaris ME220, LE220-plus和LE220R-plus; 容量:1-8个样本 说明:应用Covaris自动聚焦声学 AFA(Adaptive Focused Acoustics™)技术。具有非接触、等温、无热损伤、能量可控、重现性好、标准化、易用性、避免交叉污染和样本损失等多重优点。

货号 产品名称 品牌 购买
货号 名称 单位 购买
520225 8 联排管oneTUBE-10 AFA (12) 12排/包 咨询客服

Groundwater Capsule Supor 0.45 um 品牌:Pall


Groundwater Capsule Supor 0.45 um

品牌:Pall
CAS No.:
储存条件:室温
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

364-03211

1 pcs 咨询


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GMPS 抗体 GMPS Antibody

GMPS 抗体

GMPS Antibody

详细描述:
GMPS Antibody recognizes endogenous levels of total GMPS protein.Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GMPS protein. Antibodies are purified by protein A and peptide affinity chromatography.

应用范围:W; 反应种属:Human,Mouse,Rat; 灵敏度:Endogenous; MW (kDa):76; Isotype:Rabbit; 标记:无标记。

货号 产品名称 品牌 购买
货号 名称 单位 购买
14602S GMPS 抗体 100µl 咨询客服

Slice pH蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Slice pH
Slice pH

Slice pH

Slice pH

Slice pH

Slice pH

Products > Optimization Screens > Slice pH > Slice pH

Slice pH

Applications

  • Solubility screen, stability screen, crystallization screen
  • Preformulation screening
  • Sample buffer optimization for crystallization, cryo-electron microscopy, and NMR

Features

  • 96 pH titrated high purity 1.0 M buffer reagents
  • Vapor diffusion assay for solubility, stability & crystallization
  • pH 3.5 to 9.6 in 0.1 pH increments
  • Evaluate 20 different buffers
  • Use with ThermoFluor, Filter Plate, Dynamic Light Scattering, SEC, Native Gel, Western, Dot Blot/ELISA diagnostic assays to optimize sample solubility and stability

Description

Slice pH is a solubility screen, a stability screen, and a crystallization screen.

pH is an effective solubility, stability and crystallization variable because most proteins demonstrate pH dependent solubility minima and will solubilize, precipitate, or crystallize at particular pH values or in the presence of specific buffers. The solubility minima may correspond with the isoelectric point (pI) of the protein, but this is not always the case. The solubility minima and maxima is often complex and may depend on other chemical and physical variables in the crystallization experiment.

Using Slice pH one isolates pH, buffer type and relative supersaturation from other chemical and physical variables and to screen the effect that pH and buffer type have on the solubility, stability, homogeneity, monodispersity and crystallization of the sample. Varying the pH can alter the protonation state and charge of amino acid residues in the protein, generating different species of the protein for solubility and crystallization screening. The change in pH can have a dramatic effect on inter and intramolecular contacts in the protein and can manipulate how the protein interacts with itself, the surrounding solvent and chemicals in the drop. By screening buffer type and pH in an environment of increasing relative supersaturation, Slice pH simultaneously delivers as a solubility and a crystallization screen for proteins. After the screen, and once the appropriate sample buffer and pH are identified, the sample can be exchanged into the identified buffer and pH for optimal solubility and stability. From this point, the sample in the optimized buffer reagent can be used for crystallization trials or other assays. Further, crystallization screening and optimization experiments may be more appropriately focused on the optimal pH range and buffer type.

The Slice pH kit contains 500 microliters of 96 reagents in a V-Bottom (conical bottom) 96 Deep Well polypropylene microplate, user guide, formulations and a sealing film.

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Slice pH

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Slice pH
Slice pH
Slice pH
Slice pH

Slice pH

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Slice pH
Slice pH
Slice pH
Slice pH

CAT NO

HR2-070

NAME

Slice pH

DESCRIPTION

0.5 ml, Deep Well block format

PRICE

$276.00

cart quote

Support Material(s)

Slice pH HR2-070 Slice pH DocumentsSlice pH HR2-070 Slice pH SDSSlice pH Slice pH Formulation & Scoring Data

Related Item(S)

  • Individual Slice pH Reagents

References

1. Preparation and analysis of protein crystals. Alexander McPherson. 1982 John Wiley and Sons, Inc.

2. Increasing the size of microcrystals by fine sampling of pH limits. Alexander McPherson. J. Appl. Cryst. (1995). 28, 362-365.

3. Protein crystallization techniques, strategies and tips. Edited by Terese Bergfors. 1999. International University Line.

4. Crystallization of Biological Macromolecules. Alexander McPherson. 1991. Cold Spring Harbor Laboratory Press.

5. Current approaches to macromolecular crystallization (review). Alexander McPherson. Eur. J. Biochem. 189, 1-23 (1990).

6. Protein Isoelectric Point as a Predictor for Increased Crystallization screening efficiency. Katherine A. Kantardjieff and Bernhard Rupp. Bioinformatics (2004) 20.

7. A protein crystallization strategy using automated grid searches on successively finer grid screens. Patricia C. Weber. Methods: A Companion to Methods in Enzymology. Vol. 1, No. 1, August, pp. 31-37, 1990.

8. Two approaches to the rapid screening of crystallization conditions. Alexander McPherson. Journal of Crystal Growth 122 (1992) 161-167.

9. Optimization of buffer solutions for protein crystallization. R. A. Gosavi, T. C. Mueser and C. A. Schall. Acta Cryst. (2008). D64, 506-514.

10. Buffer Solutions The Basics. R.J. Beynon and J.S. Easterby. 1996. IRL Press.

11. Thermofluor-based optimization strategy for the stabilization and crystallization of Campylobacter jejuni desulforubrerythrin. Santos SP, Bandeiras TM, Pinto AF, Teixeira M, Carrondo MA, Romão CV. Protein Expr Purif. 2012 Feb;81(2):193-200. doi: 10.1016/j.pep.2011.10.001. Epub 2011 Oct 24.

Slice pH Slice pH

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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Wakopak Wakosil5C18N 4.6*30mm 硅胶柱5C18N 品牌:FUJIFILM Wako


Wakopak Wakosil5C18N 4.6*30mm

硅胶柱5C18N

品牌:FUJIFILM Wako
CAS No.:
储存条件:室温
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

239-54463

1 column(W) 咨询


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


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PvuI–NEB酶试剂

产品资料 – 限制性内切酶 – 限制性内切酶

PvuI                              收藏

PvuI--NEB酶试剂 PvuI--NEB酶试剂 PvuI--NEB酶试剂 PvuI--NEB酶试剂 PvuI--NEB酶试剂 PvuI--NEB酶试剂 PvuI--NEB酶试剂 PvuI--NEB酶试剂

货 号
规 格
价 格
北京库存
上海库存
广州库存
成都库存

#R0150L
2,500 units
3,259.00元

#R0150S
500 units
769.00元

#R0150V
250 units
399.00元

Download:       

识别位点

PvuI--NEB酶试剂

  • isoschizomers     |
  • compatible ends     | 
  • single letter code

在不同反应缓冲液的活性

NEBuffer 1.1: <10%
NEBuffer 2.1: 25%
NEBuffer 3.1: 100%
CutSmart Buffer: <10%

特性

重组酶、省时酶。

反应条件

NEBuffer 3.1,37℃。

浓度

10,000 units/ml。

甲基化敏感性

对哺乳动物基因组 DNA CpG 甲基化敏感。

8 联排管oneTUBE-10 AFA (120) 8 oneTUBE-10 AFA Strip Case (120)

8 联排管oneTUBE-10 AFA (120)

8 oneTUBE-10 AFA Strip Case (120)

详细描述:
8 联排管oneTUBE-10 AFA,适用于ME220 and LE220-plus。 需要Rack 500608和Waveguide 500526 用于LE220-plus 需要Rack 500609用于ME220. 可配8联排铝箔封膜520108或strip cap 8联排盖子500613 oneTUBE系列是由特殊聚合物材质合成耗材,用于实现NGS 打断建库一体化流程,在完成DNA片段化后,末端修复、接头连接以及PCR反应均可在oneTUBE一管中进行,整个文库构建过程中无需再进行样本转移, 避免了移液换液过程中的损失和污染。oneTUBE的管壁经过低吸附处理,更少可以进行10μl样本(或pg级样本)的处理,特别适合微量样本的自动化建库处理,为客户们解决文库起始量低的困难。 oneTUBE系列耗材具有以下特点: 1.便于样本追踪管理:支持条码扫描和LIMS数据自动采集; 2.自动化整合,简化工作流程:与其他实验仪器适配,流程化高通量工作 3.消除实验转移步骤,管壁低吸附处理,提高样本回收率; 4.使用AFA技术,获得精准、可重复结果; 5.DNA打断体积从10ul到50ul,浓度可达100ng/μl; 6.支持加盖和贴箔封口; 7.下游容量可达200ul,适用于各种建库体系。

适用平台:Covaris ME220, LE220-plus和LE220R-plus; 容量:1-8个样本 说明:应用Covaris自动聚焦声学 AFA(Adaptive Focused Acoustics™)技术。具有非接触、等温、无热损伤、能量可控、重现性好、标准化、易用性、避免交叉污染和样本损失等多重优点。

货号 产品名称 品牌 购买
货号 名称 单位 购买
520253 8 联排管oneTUBE-10 AFA (120) 120排/包 咨询客服

lumiprobe荧光染料BDP 630/650 azide

lumiprobe荧光染料官网代理商 水溶性荧光染料代理商 脂溶性荧光染料代理商

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  • BDP 630/650 azide

BDP 630/650 azide

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BDP 630/650 azide

BDP 630/650 azide

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BDP 630/650 azide

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25430 5 mg 210.00$

35430 10 mg 310.00$

45430 25 mg 410.00$

55430 50 mg 695.00$

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