MEKK2 抗体 MEKK2 Antibody

发表于2024年1月18日由上海金畔生物科技有限公司

MEKK2 抗体

MEKK2 Antibody

详细描述：
MEKK2 Antibody recognizes endogenous levels of total MEKK2 protein. This antibody does not cross-react with MEKK3 protein.Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly292 of human MEKK2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

应用范围：W IP；反应种属：Human；灵敏度：Endogenous； MW (kDa)：78； Isotype：Rabbit；标记：无标记。

货号	产品名称	品牌	购买

货号	名称	单位	购买
19607S	MEKK2 抗体	100µl	咨询客服

Wakopak Wakosil10C18 4.6250mm 硅胶柱10C18 4.6250mm 品牌：FUJIFILM Wako

发表于2024年1月18日由上海金畔生物科技有限公司

Wakopak Wakosil10C18 4.6*250mm

硅胶柱10C18 4.6*250mm

品牌：FUJIFILM Wako
CAS No.：
储存条件：室温
纯度：–

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
238-55251	–	1 column(D)	–	咨询	–	–

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* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

慢病毒滴度测定 qRT-PCR酶试剂盒Takara

发表于2024年1月18日由上海金畔生物科技有限公司

上海金畔生物科技有限公司代理Takara酶试剂盒全线产品，欢迎访问官网了解更多产品信息。

慢病毒滴度测定 qRT-PCR
品牌	Code No.	产品名称	包装量	价格（元）	说明书	数量
Clontech	631235	Lenti-X qRT-PCR Titration Kit	200 Rxns	￥6,010

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qRT-PCR法快速测定慢病毒滴度

· 采用qRT-PCR和TB Green技术测定病毒滴度
· 只需4 h即可完成慢病毒滴度测定
· 适用于大多数HIV-1慢病毒

Lenti-X qRT-PCR Titration Kit从收获病毒上清液到采用qRT-PCR方法完成病毒滴度的测定仅仅需要4 h，缩短了病毒收获和感染靶细胞的时间间隔，可在一天内完成收获病毒和感染靶细胞的实验过程。准确、快速地测定病毒滴度，可以确保在短时间内采用高质量的病毒感染靶细胞，并且获得可靠稳定的实验结果。利用Lenti-X qRT-PCR Titration Kit测定慢病毒滴度需要先将样品和标准品RNA (试剂盒中提供) 进行梯度稀释，然后分别进行qRT-PCR反应后绘制曲线。通过与标准曲线的Ct值比较，就可以获得样品所包含的病毒拷贝数。

*本试剂盒提供的引物用于扩增HIV-1基因组毗邻包装信号的保守区域序列，因此可以与Clontech销售的所有慢病毒载体和大多数常用慢病毒载体相兼容。

产品详情请点击：

页面更新：2019-12-23 16:13:51

壁挂型完整果蝇麻醉系统套装（终级果蝇板） Wall-Mount Flowbuddy Complet, Incl. Ultimate Flypad & Gun, 1 System/Unit

发表于2024年1月18日由上海金畔生物科技有限公司

壁挂型完整果蝇麻醉系统套装（终级果蝇板）

Wall-Mount Flowbuddy Complet, Incl. Ultimate Flypad & Gun, 1 System/Unit

详细描述：
配备有壁挂型流量调节器，麻醉枪，终极果蝇板

货号	产品名称	品牌	购买

货号	名称	单位	购买
59-122WCU	壁挂型完整果蝇麻醉系统套装（终级果蝇板）	1 System/Unit	咨询客服

iP-TEC® Cell Transport Quadruple Container #24(Main unit) iP-TEC® 24孔小室用细胞运输容器主体品牌：Sanplatec

发表于2024年1月18日由上海金畔生物科技有限公司

iP-TEC® Cell Transport Quadruple Container #24(Main unit)

iP-TEC® 24孔小室用细胞运输容器主体

品牌：Sanplatec
CAS No.：
储存条件：室温
纯度：–

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
WEB28638	–	240 pieces(24 pieces/bag×10)	–	咨询	–	–

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Lambda 核酸外切酶–NEB酶试剂

发表于2024年1月18日由上海金畔生物科技有限公司

产品资料 – DNA修饰酶与克隆技术 – 核酸酶

Lambda 核酸外切酶收藏

货号

规格

价格

北京库存

上海库存

广州库存

成都库存

#M0262L

5,000 units

3,089.00元

有

无

有

无

#M0262S

1,000 units

769.00元

有

Download:

isoschizomers |
compatible ends |
single letter code

特性

 高效 5´ →3´ 核酸外切酶活性
 切下双链 DNA 的 5´ 单核苷酸

概述

Lambda 核酸外切酶作用于双链 DNA，沿 5´→3´ 方向逐步切去 5´ 单核苷酸。最适底物是 5´ 磷酸化的双链 DNA，也能缓慢降解单链 DNA 和非磷酸化底物。该酶不能从 DNA 的切刻或缺口处起始消化。

来源

E. coli 的 Lambda 核酸外切酶基因与编码麦芽糖结合蛋白（MBP）的基因融合表达。亲和层析后，从融合蛋白上切下 Lambda 核酸外切酶，并纯化除去 MBP。

反应条件

1X Lambda 核酸外切酶反应缓冲液

[67 mM Glycine-KOH（pH 9.4 @ 25℃），2.5 mM MgCl₂，50 μg/ml BSA]，37℃ 温育。
热失活：75℃ 加热 10 分钟。

质保声明

Lambda 核酸外切酶经过严格的质控检测，确保该产品具有最高的活性和纯度。详情请登陆 www.neb.com 或 www.neb-china.com。

单位定义

1 单位指在 50 μl 反应体系中，37℃ 条件下，30 分钟内能从双链 DNA 底物上催化产生 10 nmol 酸溶性脱氧核糖核苷酸所需的酶量。活性检测条件请登陆 www.neb.com 或 www.neb-china.com。

浓度

5,000 units/ml。

注意事项

5´ -0H 端的切割速度比 5´ -PO₄ 端慢 20 倍。单链 DNA 比双链 DNA 慢 100 倍。

参考文献

关于该酶性质和产品应用的参考文献，请登陆 www.neb.com 或 www.neb-china.com。

Presep(R) Column Adapter Packing 再灌装Presep Column Adapter用的铁氟龙密封（白色）品牌：FUJIFILM Wako

发表于2024年1月18日由上海金畔生物科技有限公司

Presep(R) Column Adapter Packing

再灌装Presep Column Adapter用的铁氟龙密封（白色）

品牌：FUJIFILM Wako
CAS No.：
储存条件：室温
纯度：–

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
298-50051	–	5pcs	–	咨询	–	–

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非磷酸化 (Active) β-Catenin (Ser45) (D2U8Y) XP® 兔单克隆抗体 Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb

发表于2024年1月18日由上海金畔生物科技有限公司

非磷酸化 (Active) β-Catenin (Ser45) (D2U8Y) XP® 兔单克隆抗体

Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb

详细描述：
Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb recognizes endogenous levels of β-catenin protein only when Ser45 is not phosphorylated.Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser45 of human β-catenin protein.

应用范围：W IP IHC-P IF-F IF-IC F；反应种属：Human,Mouse,Rat,Monkey,Zebrafish,Dog；灵敏度：Endogenous； MW (kDa)：92； Isotype：Rabbit IgG；标记：无标记。

货号	产品名称	品牌	购买

货号	名称	单位	购买
19807T	非磷酸化 (Active) β-Catenin (Ser45) (D2U8Y) XP® 兔单克隆抗体	20 µl	咨询客服
19807S	非磷酸化 (Active) β-Catenin (Ser45) (D2U8Y) XP® 兔单克隆抗体	100µl	咨询客服

lumiprobe荧光染料Azide-PEG4-Amine

发表于2024年1月18日由上海金畔生物科技有限公司

lumiprobe荧光染料官网代理商水溶性荧光染料代理商脂溶性荧光染料代理商

Products
Miscellaneous reagents
Bifunctional crosslinkers
Azide-PEG4-Amine

Azide-PEG4-Amine

Azides Amines Click chemistry Cross linking reagents PEG4 (tetraethylene glycol)

Bulk inquiry Azide-PEG4-Amine

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Cat. #	Quantity	Price	Lead time	Buy this product
1868-1g	1 g	90.00$
1868-5g	5 g	250.00$
1868-25g	25 g	950.00$

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Quantity

Price

Lead time

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1868-1g

1 g

90.00$

1868-5g

5 g

250.00$

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25 g

950.00$

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Oligoethylene glycols are versatile hydrophilic linkers. Each monomer has a length of 3.43 Å, so that the length of PEG4 is around 14 Å (1.4 nm).

Azide-PEG4-Amine is a bifunctional compound with an amine group that can be modified by acylation by activated esters or similar carbonyl compounds, and an azide group that is suitable for Click chemistry reactions. PEG4 is highly hydrophilic and biocompatible.

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D-甘露醇/L-阿拉伯糖醇检测试剂盒 D-Mannitol/L-Arabitol Assay Kit 货号：K-MANOL Megazyme试剂盒

发表于2024年1月18日由上海金畔生物科技有限公司

D-甘露醇/L-阿拉伯糖醇检测试剂盒

英文名：D-Mannitol/L-Arabitol Assay Kit

货号：K-MANOL

规格：60 assays (manual) / 600 assays

市场价： 3900元

分析物意义：食品中常见的增甜剂有阿斯巴甜、D-甘露醇、D-山梨醇和木糖醇

Megazyme检测试剂盒优点：K-MANOL-方法新颖/只有试剂盒可用

The D-Mannitol/L-Arabitol test kit is suitable for the rapid and specific measurement and analysis of D-mannitol and L-arabitol in food, beverages and other materials.
Suitable for manual, auto-analyser and microplate formats.
Data calculators are located in the Technical Resources tab.

UV-method for the determination of D-Mannitol and L-Arabitol
in foodstuffs and other materials

Principle:
(mannitol dehydrogenase)
(1) D-Mannitol + NAD⁺ → D-fructose + NADH + H⁺

(mannitol dehydrogenase)
(2) L-Arabitol + NAD⁺ → L-xylulose + NADH + H⁺

Kit size: 60 assays (manual) / 600 (microplate)
/ 580 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 6 min
Detection limit: 0.50 mg/L
Application examples:
Wine, chewing gum, dietetic foods, candies, cosmetics, pharmaceuticals
and other materials (e.g. biological cultures, samples, etc.)
Method recognition: Novel method

Advantages

Novel product (only enzymatic kit available)

Very cost effective

All reagents stable for > 2 years after preparation

Simple format

Rapid reaction

Mega-Calc™ software tool is available from our website for hassle-free raw data processing

Standard included

Extended cofactors stability

Suitable for manual, microplate and auto-analyser formats

Q1. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.

Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.

State the kit lot number being used (this is found on the outside of the kit box).

State which assay format was used (refer to the relevant page in the kit booklet if necessary).

State exact details of any modifications to the standard procedure that is provided by Megazyme.

State the sample type and describe the sample preparation steps if applicable.

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q3. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q4. Can perchloric acid be used to deproteinise / clarify samples prior to analysis using the D-Mannitol/L-Arabitol Assay Kit? If so, how should such an extraction be performed?

Yes. Perchloric acid extraction can be used in conjunction with this kit, and should be performed as follows:
WARNING: If you have not worked with perchloric acid before, you must consult your safety officer for advice. Also, depending on the nature of the samples, it may be possible to reduce the concentration of perchloric acid, to for example 0.3 M (i.e. in the case of plasma). It is thus very important to determine if this is possible for each type of sample used, in order to reduce the risk from working with concentrated perchloric acid.

Liquid samples:

Carefully add an equal volume of ice cold 3 M perchloric acid and homogenise / fully disperse the sample (as appropriate).

After 15 min incubation on ice (or in a refrigerator), centrifuge at 3000 x g for 15 min at 4˚C.

Neutralise by the slow addition of 2 M KOH.

Incubate on ice (or in a refrigerator) until the potassium perchlorate has settled out by gravity (approximately 10 min), and then simply remove some of the clear supernatant and use directly in the assay.

Solid samples:

Accurately weigh approx. 5 g of homogenised sample into a beaker containing 20 mL of 1 M perchloric acid and very carefully homogenise with an Ultraturrax® (or equivalent) for 5 min.

Carefully add approx. 40 mL of distilled water and neutralise using 2 M KOH (using pH test strips for example). Quantitatively transfer the contents to a 100 mL volumetric flask and fill to the mark with distilled water. If a fat layer develops, make sure this is above the mark, and the aqueous layer is at the mark.

Incubate in a refrigerator for approx. 20 min to allow separation of fat and precipitation of potassium perchlorate.

Filter through Whatman No. 1 filter paper, discarding the first few mL of filtrate, and use directly in the assay.

Q5. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q6. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q7. Can oligosaccharides or polysaccharides be measured using the kit assay?

The kit assay will only measure the non-covalently linked monosaccharide.

Oligosaccharides or polysaccharides can be measured after hydrolysis to the monosaccharide. Generally acid hydrolysis can be achieved by boiling the oligo/polysaccharide in 1.3 M HCl for 1 h. It is recommended that scientific literature is consulted for information on hydrolysis conditions for the particular oligo/polysaccharide that is being measured.

Q8. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q9. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q10. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q11. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.

Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).

Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q12. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q13. Is it possible to add a larger volume then 2 μL of enzyme to the microplate assay? In some instances 2 μL can be difficult to pipette manually.

Yes, instead of adding 2 μL of enzyme suspension an alternative is to dilute the enzyme and add a larger volume to the microplate assay.

Dilute the assay buffer 10-fold with distilled water and use this as the diluent to dilute an aliquot of the enzyme suspension also by 10-fold. Instead of 2 μL, use 20 μL of the diluted enzyme in the microplate assay.

Q14. Can the sensitivity of the kit assay be increased?

Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.

Q15. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.

HEPES缓冲液 HEPES Buffer salt 250 grams 货号：B-HEPES250 Megazyme试剂盒

发表于2024年1月18日由上海金畔生物科技有限公司

HEPES缓冲液 英文名：HEPES Buffer salt 250 grams 货号：B-HEPES250 规格：250g 市场价： 2100元 HEPES缓冲液 暂无问题解答 暂无视频

壁挂型完整果蝇麻醉系统套装（标准果蝇板） WallMount Flowbuddy Complete, Includes Flowbed & Blowgun, 1 System/Unit

发表于2024年1月18日由上海金畔生物科技有限公司

壁挂型完整果蝇麻醉系统套装（标准果蝇板）

WallMount Flowbuddy Complete, Includes Flowbed & Blowgun, 1 System/Unit

详细描述：
配备有壁挂型流量调节器，麻醉枪，标准果蝇板

货号	产品名称	品牌	购买

货号	名称	单位	购买
59-122WC	壁挂型完整果蝇麻醉系统套装（标准果蝇板）	1 System/Unit	咨询客服

4-甲基β-laminaritrioside 4-Methylumbelliferyl-β-laminaritrioside 货号：O-4MULAM3 Megazyme试剂盒

发表于2024年1月18日由上海金畔生物科技有限公司

4-甲基β-laminaritrioside

英文名：4-Methylumbelliferyl-β-laminaritrioside

货号：O-4MULAM3

规格：10 mg

市场价： 5800元

Synonym: MU-β-laminaritrioside,
4MU-β-laminaritrioside
CAS: 618879-30-4
Molecular Formula: C₂₈H₃₈NO₁₈Molecular Weight: 662.6
Purity: > 98%

High purity 4-Methylumbelliferyl-β-laminaritrioside for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This is a flourimetric substrate for the measurement/detection of 1,3-β-glucan degrading enzymes.

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LABTECH TF-20 Digestion tubes-cover 100mL PTFE 莱伯泰科TF-20消解罐-盖子 100mL PTFE 品牌：SONSAN

发表于2024年1月18日由上海金畔生物科技有限公司

LABTECH TF-20 Digestion tubes-cover 100mL PTFE

莱伯泰科TF-20消解罐-盖子 100mL PTFE

品牌：SONSAN
CAS No.：
储存条件：室温
纯度：–

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
XJ1006-B	–	个	–	咨询	–	–

* 干冰运输、大包装及大批量的产品需酌情添加运输费用

Beta葡萄糖醛酸酶[大肠杆菌] Beta-Glucuronidase (E.coli) 货号：E-BGLAEC Megazyme试剂盒

发表于2024年1月18日由上海金畔生物科技有限公司

Beta葡萄糖醛酸酶[大肠杆菌]

英文名：Beta-Glucuronidase (E.coli)

货号：E-BGLAEC

规格：500000 Units

市场价： 3600元

High purity recombinant beta-Glucuronidase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.31
CAZy Family: GH2

Recombinant. From Escherichia coli. Supplied in solution (Tris.HCl / NaCl / EDTA).
Hydrolysis of non-reducing terminal β-D-glucuronic acid residues from glycoproteins and oligosaccharides of glycoconjugates.

Specific activity: ~ 35,000 U/mg (37^oC, pH 6.8 on phenolphthalein-β-D-glucuronide);
~ 100 U/mg (37^oC, pH 7.5 on pNP-β-D-glucuronide).

Store at 4^oC.
Do Not Freeze/Thaw

Custom quantities and formulations available up on request.

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Ph.D.-C7C 噬菌体展示肽库试剂盒–NEB酶试剂

发表于2024年1月18日由上海金畔生物科技有限公司

产品资料 – 糖生物学与蛋白质工具 – 噬菌体展示

Ph.D.-C7C 噬菌体展示肽库试剂盒收藏

货号

规格

价格

北京库存

上海库存

广州库存

成都库存

#E8120S

10 次淘选实验

7,639.00元

有

无

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isoschizomers |
compatible ends |
single letter code

Ph.D.试剂盒组成

– 10 次独立淘选实验的足量噬菌体展示库，10⁹ 个克隆

– 28 gIII 测序引物（100 pmol）

– 96 gIII 测序引物（100 pmol）

– E. coli 宿主菌 ER2738

– 对照实验靶分子（链霉亲和素）和洗脱剂（生物素）

– 详细的使用手册

建议测序引物序列（不单独提供）如下：

-28 gIII 测序引物 (22-mer)

5´d(GTATGGGATTTTGCTAAACAAC)3´

-96 glll 测序引物 (20-mer)

5´d(CCCTCATAGTTAGCGTAACG)3´

概述

噬菌体展示是一种体外筛选技术。它可以将一系列多肽或蛋白质文库展示在噬菌体颗粒的外部，而编码这些蛋白的 DNA 则位于病毒颗粒内部。利用展示蛋白与其编码 DNA 之间的联系，我们可以通过一种简单的称为“生物淘选”的体外筛选方法，快速筛选出给定靶分子（抗体、酶、细胞表面受体等）的亲和配体。最简单的淘选方法是（见图 1）：将展示不同多肽的噬菌体文库与固定在平板或小珠上的靶分子孵育，先洗去未结合的噬菌体，而后洗脱特异性结合的噬菌体。扩增洗脱下的噬菌体。重复上述结合扩增过程，使与靶分子结合的多肽序列的噬菌体得到富集。经过 3-4轮筛选后，每个克隆通过 DNA 测序或 ELISA 鉴定。

NEB 提供 3 种随机肽库和供自制肽库所需的 M13KE克隆展示载体。这 3 种肽库包括 7 肽库（Ph.D.-7）、12 肽库（Ph.D.-12）和含有二硫键的环 7 肽库（Ph.D.-C7C）。Ph.D.-C7C 随机肽库所展示的多肽两侧各加了一个半胱氨酸。噬菌体组装时经过氧化，形成环化的多肽。所有肽库的容量超过 20 亿个克隆。上述 3 种肽库表达的随机肽均在噬菌体次要包膜蛋白 pIII 的 N 端，故每一个病毒粒子可以表达 5 个拷贝。

展示在噬菌体表面的随机肽库已被应用于多种领域，包括绘制抗原表位图谱（图 2）、研究蛋白质与蛋白质相互作用及酶抑制剂，并且还用于发现 GroEL、HIV、半导体表面、小分子荧光团和药物的肽配基。

参考文献

有关该类产品特性和应用的相关文献请登陆 www.neb-china.com，www.neb.com。

C-FLEX 374-250-2 品牌：Saint-Gobain

发表于2024年1月18日由上海金畔生物科技有限公司

C-FLEX 374-250-2

品牌：Saint-Gobain
CAS No.：
储存条件：室温
纯度：–

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
301-98911	–	1 roll	–	咨询	–	–

* 干冰运输、大包装及大批量的产品需酌情添加运输费用

葡萄糖苷酸酶检测底物 Aldouronic Acid Mixture (Reduced) 货号：O-AMXR Megazyme试剂盒

发表于2024年1月18日由上海金畔生物科技有限公司

葡萄糖苷酸酶检测底物 英文名：Aldouronic Acid Mixture (Reduced) 货号：O-AMXR 规格：100 mg 市场价： 2800元 High purity Aldouronic Acids Mixture (NaBH₄ Reduced) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. Contains a mixture of borohydride reduced aldobiouronic, aldotriouronic and aldotetraouronic and aldopentaouronic acids (10:60:20:10). Substrate for α-glucuronidase. 暂无问题解答 暂无视频

MUC1 (D9O8K) XP® 兔单克隆抗体 (PE 偶联) MUC1 (D9O8K) XP® Rabbit mAb (PE Conjugate)

发表于2024年1月18日由上海金畔生物科技有限公司

MUC1 (D9O8K) XP® 兔单克隆抗体 (PE 偶联)

MUC1 (D9O8K) XP® Rabbit mAb (PE Conjugate)

详细描述：
MUC1 (D9O8K) XP® Rabbit mAb (PE Conjugate) detects endogenous levels of total MUC1 protein.This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated MUC1 (D9O8K) XP® Rabbit mAb #14161.

应用范围：F；反应种属：Human；灵敏度：Endogenous；Isotype：Rabbit IgG；标记：PE。

货号	产品名称	品牌	购买

货号	名称	单位	购买
19926S	MUC1 (D9O8K) XP® 兔单克隆抗体 (PE 偶联)	100µl	咨询客服

新型钙离子荧光探针Calbryte 520, AM 细胞渗透性货号20653-AAT Bioquest荧光染料

发表于2024年1月18日由上海金畔生物科技有限公司

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

新型钙离子荧光探针Calbryte 520, AM *细胞渗透性*

新型钙离子荧光探针Calbryte 520, AM 细胞渗透性

货号	20653	存储条件	在零下15度以下保存, 避免光照
规格	1 mg	价格	5136
Ex (nm)	493	Em (nm)	515
分子量	1090.90	溶剂	DMSO
产品详细介绍

简要概述

新型钙离子荧光探针Cal-520 AM是美国AAT Bioquest生产的钙离子荧光探针，Calbryte 520，Calbryte 590和Calbryte 630提供最强大的均匀荧光测定工具，用于检测细胞内钙动员。与现有的钙指示剂（如Fluo-3 AM，Fluo-4 AM和Rhod-2 AM）相比，这些钙敏感染料明显更亮，并提供更高的信噪比，大大提高了细胞内保留和负载效率。表达通过钙发出信号的GPCR或钙通道的细胞可以预装载可以穿过细胞膜的Calbryte 520 AM，Calbryte 590 AM或Calbryte 630 AM。一旦进入细胞内，Cal520 AM，Calbryte 590 AM或Calbryte 630 AM的亲脂性阻断AM基团被细胞内酯酶切割，产生带负电荷的荧光Calbryte 染料，留在细胞内。它们与钙结合后的荧光大大增强。当用激动剂刺激细胞时，该受体表示细胞内钙的释放，这显着增加了Calbryte 520，Calbryte 590或Calbryte 630的荧光。高灵敏度和> 100倍荧光增强的特性使Calbryte 520 AM， Calbryte 590 AM或Calbryte 630 AM是测量细胞内钙的最强大指标。

除了方便的激发波长和钙的大荧光增强外，Calbryte 520，Calbryte 590和Calbryte 630主要定位于细胞溶胶中，不像Rhod-2主要位于线粒体中。此外，Calbryte 590和Calbryte 630的长Ex / Em波长使这些染料成为完美的钙指示剂，可与绿色荧光蛋白（GFP）细胞系进行多色检测。此外，Calbryte 520，Calbryte 590或Calbryte 630钙测定经过优化，可与大多数现有荧光仪器兼容。 Calbryte 520可在488 nm处激发，并与FITC滤光片组配合使用。 Calbryte 590经过优化，可在555 nm激发，并与TRITC / Cy3滤光片组配合使用。 Calbryte 630经过优化，可在594 nm激发，并与TexasRed®滤光片组配合使用。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的钙离子荧光探针。

点击查看光谱

点击查看实验方案

钙离子篇：时间轴式讲解应用于钙离子检测的探针

适用仪器

流式细胞仪
激发：	488nm激光
发射：	530/30nm滤波片
通道：	FITC

荧光显微镜
激发：	FITC
发射：	FITC
推荐孔板：	黑色透明

荧光酶标仪
激发：	490nm
发射：	525nm
cutoff:	515nm
推荐孔板：	黑色透明
读取模式：	底读模式

产品说明书

使用Calbryte 520 AM，Calbryte 590 AM或Calbryte 630 AM酯类

1.操作步骤

Calbryte AM酯应在使用前在无水DMSO中重新配制。 DMSO储备溶液可以在-20℃下储存（干燥）并避光。在这些条件下，AM酯应稳定三个月。以下是我们推荐的将Calbryte 520 AM，Calbryte 590 AM或Calbryte 630 AM酯加入活细胞的方案。该方案仅提供指南，实际应根据您的特定需求进行修改。

a）在无水DMSO中制备2至5mM Calbryte 520AM，Calbryte 590AM或Calbryte 630AM酯的储备溶液。

b）将Calbryte 520 AM，Calbryte 590 AM或Calbryte 630 AM溶解在DMSO中或将等份的指示剂储备溶液解冻至室温。在Hanks和Hepes缓冲液（HHBS）或您选择的缓冲液（0.04％Pluronic®F-127）中制备10至20μM的染料工作溶液。细胞加载所需指示剂的确切浓度必须根据经验确定。

注意：非离子型洗涤剂Pluronic®F-127有时用于增加Calbryte 520 AM，Calbryte 590 AM或Calbryte 630 AM酯的水溶性。各种Pluronic®F-127可从金畔购买。

c）如果您的细胞（如CHO细胞）含有有机阴离子转运蛋白，可将丙磺舒（1-2 mM）添加到染料工作溶液中（最终浓度为0.5-1 mM）

注意：各种ReadiUse 丙磺舒（包括水溶性钠盐和稳定溶液）均可从金畔购买。

d）将等体积的染料工作溶液（来自步骤b或c）加入细胞板中。

e）将染料加载板在细胞培养箱中孵育约60分钟，然后将板在室温下再孵育15分钟。

f）用HHBS或您选择的含有阴离子转运蛋白抑制剂（如1 mM丙磺舒）的缓冲液替换染料工作溶液，以去除多余的探针。

g）对于Calbryte 520 AM，在Ex / Em = 490 / 525nm处进行钙测试，对于Calbryte 590 AM，使用540/590nm进行钙测试，对于Calbryte 630 AM，使用610/640nm进行钙测试。

2.测量细胞内钙响应：

图1. CHO-K1细胞中内源性P2Y受体对ATP的反应。将CHO-K1细胞以每孔100μL每孔40,000个细胞接种过夜，在96孔黑色壁/透明底板中。将含有丙磺舒的HHBS中的100μLFluo-4AM或Calbryte TM 520AM加入孔中，并将细胞在37℃下孵育45分钟。用200μLHHBS替换染料加载培养基，加入50μL50μMATP，并使用FITC通道用荧光显微镜（Keyence）成像。

新型钙离子荧光探针Calbryte 520, AM *细胞渗透性* 货号20653

图2.用Calbryte 520或Fluo-4 AM测量的CHO-M1细胞中外源M1受体的卡巴胆碱刺激的钙响应。在96孔黑壁/透明底板中，将CHO-M1细胞以每100μL/孔40,000个细胞接种过夜。将100μLFluo-4AM或不含丙磺舒的Calbryte 520AM加入细胞中，并将细胞在37℃下孵育45分钟。通过FlexStation 3（Molecular Devices）添加Carbachol（50μL/孔）以达到最终指示的浓度。

新型钙离子荧光探针Calbryte 520, AM *细胞渗透性* 货号20653

图3. CHO-K细胞中内源性P2Y受体对ATP的反应。在96孔黑壁/透明底板中，将CHO-K细胞以每100μL/孔40,000个细胞接种过夜。将100μL具有2mM丙磺舒的HbBS中的Calbryte 590AM或Calbryte 630 AM加入孔中，并将细胞在37℃下孵育1小时。将染料加载培养基替换为200μLHHBS，用50μL50μMATP处理，并使用TRITC通道（Calbryte 590）和Texas Red Channel（Calbryte 630）用荧光显微镜（Keyence）成像。

结论

由于Ca²⁺在生物学中的重要性，已经建立了许多用于分析细胞和/或亚细胞Ca²⁺活性机制的技术/方法。尽管用于分析Ca ²⁺活性的每种方法都具有优于其他方法的某些优点，但每种方法也存在缺点。凭借上述出色的性能，我们相信Calbryte 520，Calbryte 590和Calbryte 630钙检测试剂为各种生物系统中的细胞内钙分析和监测提供了新的强大工具。

正如可能预测的那样，Ca^{2 +}分析中许多研究人员的兴趣从细胞水平转移到亚细胞水平。已经发现Ca^{2 +}在整个细胞中均匀分布，并且在多种细胞（例如，卵母细胞，心肌细胞，肝细胞和外分泌细胞）中观察到Ca^{2 +}的细胞内异质性（例如Ca²⁺ waves and Ca²⁺ sparks）。随着20世纪80年代共聚焦激光扫描显微镜（CLSM）和2000年代先进的酶标仪（如FLIPR，FDSS和NOVOStar专用于细胞内Ca^{2 +}检测）的出现，细胞内Ca^{2 +}的测量显着加速。共聚焦激光扫描显微镜和最近的多光子显微镜除了测量其浓度外，还允许在亚细胞水平上对细胞内Ca²⁺信号传导进行精确的空间和时间分析。

参考文献

Behavioral role of the reciprocal inhibition between a pair of Mauthner cells during fast escapes in zebrafish
Authors: Takashi Shimazaki, Masashi Tanimoto, Yoichi Oda, Shin-ichi Higashijima
Journal: Journal of Neuroscience (2019): 1182–1194

Deep Two-Photon Imaging In Vivo with a Red-Shifted Calcium Indicator
Authors: Antje Birkner, Arthur Konnerth
Journal: (2019): 15–26

In Vivo Functional Mapping of a Cortical Column at Single-Neuron Resolution
Authors: Carsten H Tischbirek, Takahiro Noda, Manabu Tohmi, Antje Birkner, Israel Nelken, Arthur Konnerth
Journal: Cell reports (2019): 1319–1326

Multiplex imaging of quantal glutamate release and presynaptic Ca2+ at multiple synapses in situ
Authors: Thomas P Jensen, Kaiyu Zheng, Nicholas Cole, Jonathan S Marvin, Loren L Looger, Dmitri A Rusakov
Journal: bioRxiv (2019): 336891

Multiplex imaging relates quantal glutamate release to presynaptic Ca 2+ homeostasis at multiple synapses in situ
Authors: Thomas P Jensen, Kaiyu Zheng, Nicholas Cole, Jonathan S Marvin, Loren L Looger, Dmitri A Rusakov
Journal: Nature Communications (2019): 1414

A 3D human triculture system modeling neurodegeneration and neuroinflammation in Alzheimer’s disease
Authors: Joseph Park, Isaac Wetzel, Ian Marriott, Didier Dréau, Carla D’Avanzo, Doo Yeon Kim, Rudolph E Tanzi, Hansang Cho
Journal: Nature neuroscience (2018): 941

Concepts of All-Optical Physiology
Authors: Jan Doering, Ting Fu, Isabelle Arnoux, Albrecht Stroh
Journal: (2018): 153–174

Optical probes for neurobiological sensing and imaging
Authors: Eric H Kim, Gregory Chin, Guoxin Rong, Kira E Poskanzer, Heather A Clark
Journal: Accounts of chemical research (2018): 1023–1032

Optogenetic Control of Voltage-Gated Calcium Channels
Authors: Guolin Ma, Jindou Liu, Yuepeng Ke, Xin Liu, Minyong Li, Fen Wang, Gang Han, Yun Huang, Youjun Wang, Yubin Zhou
Journal: Angewandte Chemie International Edition (2018): 7019–7022

α V β 3 Integrin regulates astrocyte reactivity
Authors: Raúl Lagos-Cabré, Alvaro Alvarez, Milene Kong, Francesca Burgos-Bravo, Areli Cárdenas, Edgardo Rojas-Mancilla, Ramón Pérez-Nunez, Rodrigo Herrera-Molina, Fabiola Rojas, Pascal Schneider
Journal: Journal of Neuroinflammation (2017): 194

相关产品

产品名称	货号
钙离子荧光探针Cal-520 , AM	Cat#21130
钙离子荧光探针Fluo-8, AM	Cat#21080
新型钙离子荧光探针Calbryte 590, AM 细胞渗透性	Cat#20700

说明书

新型钙离子荧光探针Calbryte 520, AM *细胞渗透性*.pdf

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Wakopak Wakosil10C18 4.6*250mm

硅胶柱10C18 4.6*250mm

iP-TEC® Cell Transport Quadruple Container #24(Main unit)

iP-TEC® 24孔小室用细胞运输容器主体

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特性

概述

来源

反应条件

质保声明

单位定义

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注意事项

参考文献

Presep(R) Column Adapter Packing

再灌装Presep Column Adapter用的铁氟龙密封（白色）

Azide-PEG4-Amine

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Q2. Should the pH of the sample be adjusted even for samples in acidic media?

Q3. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Q4. Can perchloric acid be used to deproteinise / clarify samples prior to analysis using the D-Mannitol/L-Arabitol Assay Kit? If so, how should such an extraction be performed?

Q5. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Q6. I have some doubts about the appearance/quality of a kit component what should be done?

Q7. Can oligosaccharides or polysaccharides be measured using the kit assay?

Q8. Can the sensitivity of the kit assay be increased?

Q9. How much sample should be used for the clarification/extraction of my sample?

Q10. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

Q11. Can the manual assay format be scaled down to a 96-well microplate format?

Q12. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

Q13. Is it possible to add a larger volume then 2 μL of enzyme to the microplate assay? In some instances 2 μL can be difficult to pipette manually.

Q14. Can the sensitivity of the kit assay be increased?

Q15. Must the minimum absorbance change for a sample always be at least 0.1?

LABTECH TF-20 Digestion tubes-cover 100mL PTFE

莱伯泰科TF-20消解罐-盖子 100mL PTFE

Ph.D.-C7C 噬菌体展示肽库试剂盒 收藏

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Ph.D.-C7C 噬菌体展示肽库试剂盒收藏

新型钙离子荧光探针Calbryte 520, AM 细胞渗透性