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生物素-11-dGTP
货号 | 17015 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 25 nmoles | 价格 | 1200 | |
Ex (nm) | Em (nm) | |||
分子量 | 898.71 | 溶剂 | Water | |
产品详细介绍 |
简要概述
产品基本信息
货号:17015
产品名称:生物素-11-dGTP
规格:25 nmoles
储存条件:-15℃避光防潮
保质期:24个月
产品物理化学光谱特性
分子量:898.71
溶剂:水
产品介绍
生物素修饰的脱氧鸟苷5′-三磷酸酯广泛用于各种非放射性DNA标记反应,包括切口平移,随机引物标记,cDNA标记和3′-末端标记。可以通过切口平移,随机引物,3′-末端末端标记或PCR的方法将生物素11-dGTP酶促地掺入DNA中。数字“ 11”是dGTP与生物素之间连接子骨架中的碳原子数。连接子越长,生物素与抗生物素蛋白的相互作用越有效。另一方面,接头越短,dGTP就越有效地掺入DNA。建议链接器’11’的长度对于大多数应用而言是最佳的。 Biotin-11-dGTP用于在各种杂交应用中产生生物素化的DNA探针,包括Southern印迹,Northern印迹,斑点印迹,固定细胞和组织。它在化学上等同于PerkinElmer(PE)的NEL541001EA。
参考文献
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