CellEase^® II DNA提取试剂

DNA extraction reagent : CellEase^® II series

 

 

 

 

◆原理

 

此款试剂盒用于细胞内 DNA 提取，操作极为简便，提取的 DNA 可直接用于下游PCR 操作，无需纯化步骤，仅需 9 分钟。CellEase^® II 跟其他产品相比无需洗脱，更可提高回收效率。方法简单，只需将样品与 CellEase A和B混合，并进行2步温控孵育即可。

该试剂盒可从动物组织、微生物、植物细胞等样品中制备 DNA。该试剂盒不仅用于常规实验室，也可用在食品检测方面。

 

 

◆优点・特色

 

　● 快速高效提取DNA

　● 简单操作即可制备PCR的DNA模板

　● 成本低

　● 用于实验室和食品检测

 

DNA 提取试剂 CellEase^® II 系列产品

 

 

 

 

◆案例・应用

【实验步骤】

 

 

　● 实验前，请先准备 CellEase^® 混合液，按 1：1：3 的比例混合溶液 A、B 和 ddH₂O。

　● 将样品切成合适的大小，放入离心管中。

　● 向管中加入 CellEase^® 混合液。

　● 72℃下孵育样品 6 分钟。

　● 接着在 94℃条件下孵育样品 3分钟。

　● 即可得到纯化 DNA，取适量作为模板，进行 PCR实验。

 

【实验案例】

 

<小鼠尾巴DNA 提取与检测>

 

M ：Marker   (100bp ladder)

1　：CellEaseII,   Extracted solution was undiluted

2　：CellEaseII,   Extracted solution was ×10 diluted

3　：CellEaseII,   Extracted solution was ×100 diluted

4　：Conventional   CellEase , Extracted solution was undiluted

5　：Conventional   CellEase , Extracted solution was ×10 diluted

6　：Conventional   CellEase , Extracted solution was ×100 diluted

 

<全血DNA 提取与检测>

 

Sample: Whole blood (EDTA)

M: Marker (100 bp ladder)

1: CellEase^® Blood

2: CellEase^® Blood

3: CellEase^® Blood

4: Extraction by water

 

<金黄色葡萄球菌DNA提取与检测>

 

 

M Marker (500 bp ladder) 1: Undiluted   Sample Add 5 μl of DNA extract to PCR 2: x 10 dilution 3: x 102 dilution 4: x 103 dilution 5: x 104 dilution

6: Undiluted Sample Add 6 μl of DNA extract to PCR 7: x 10 dilution 8: x 102 dilution 9: x 103 dilution 10: x 104 dilution

<番茄叶DNA提取与检测>

 

M: Marker（500 bp ladder） 1: + CellEase®,   Without dilution Add 5 μl to PCR 2: + CellEase®,   ×10 dilution Add 5 μl to PCR 3: + CellEase®,   × 100 dilution Add 5 μl to PCR 4: – CellEase®,   Without dilution Add 5 μl to PCR 5: – CellEase®,   ×10 dilution Add 5 μl to PCR 6: – CellEase®,   × 100 dilution Add 5 μl to PCR 7: + CellEase®,   Without dilution Add 6 μl to PCR 8: + CellEase®,   ×10 dilution Add 6 μl to PCR 9: + CellEase®,   × 100dilution Add 6 μl to PCR

10: – CellEase®，Without dilution Add 6 μl to PCR 11: – CellEase®，×10 dilution Add 6 μl to PCR 12: – CellEase®，×100 dilution Add 6 μl to PCR 13: + CellEase®，Without dilution Add 7 μl to PCR 14: + CellEase®，×10 dilution Add 7 μl to PCR 15: + CellEase®，×100 dilution Add 7 μl to PCR 16: – CellEase®，Without dilution Add 7 μl to PCR 17: – CellEase®，×10 dilution Add 7 μl to PCR 18: – CellEase®，×100 dilution Add 7 μl to PCR

 

<金枪鱼DNA提取与检测>

 

M: Marker（100 bp ladder）

1: Sample 6 mg,   Add 5 μl of DNA extract to PCR

2: Sample 6 mg,   Add 6 μl of DNA extract to PCR

3: Sample 6 mg,   Add 7 μl of DNA extract to PCR

4: Sample 9 mg,   Add 5 μl of DNA extract to PCR

5: Sample 9 mg,   Add 6 μl of DNA extract to PCR

6: Sample 9 mg,   Add 7 μl of DNA extract to PCR

7: Sample 18 mg,  Add 5 μl of DNA extract to PCR

8: Sample 18 mg   Add 6 μl of DNA extract to PCR

9: Sample 18 mg   Add 7 μl of DNA extract to PCR