NLRP3抗体 anti-NLRP3/NALP3, mAb

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  • 参考文献

NLRP3抗体NLRP3抗体                              anti-NLRP3/NALP3, mAb



产品描述

  炎性体是介导caspase-1活化的多蛋白复合物, 它可以促进除了别的以外的促炎细胞因子白介素(IL)-1β和 IL-18的分泌物。nod样受体家族的 NLRP3/NALP3是发危险信号到caspase-1活化炎性体的关键组成部分。NLRP3缺陷是导致家族性寒冷型自身炎症综合征1型(FCAS1)、Muckle-Wells 综合征 (MWS) 和慢性炎症性神经,皮肤,关节综合征的原因。

规格 & 操作

产品数据

产品别名

NACHT-, LRR- and PYD-containing Protein 3; NLRP3; Cryopyrin;
Cold Autoinflammatory Syndrome 1 Protein

抗小鼠NLRP3/NALP3单抗(Cryo-1)

NLRP3/NALP3单抗(Cryo-2)

特性

集落

Cryo-1

Cryo-2

同型

小鼠IgG2b

来源/宿主

纯化浓缩的杂交瘤细胞组织培养上清液。

应用

Western印迹:(1 μg/mL)

Western印迹:(1 μg/mL)

免疫细胞化学:1. see J. Virol. 85, 13019 (2011)

                        2. Nat. Immunol. 13, 255 (2012)

免疫沉淀:in RIPA buffer, see Mol. Cell 49, 331(2012)

免疫组化:1:200, for a protocol see Nature 493,  67 (2012)

ChIP assay:2 µg/DNA of 10E6 cells

                   (see Nat. Immunol. 16, 859 (2015))

交叉反应性

小鼠

人,小鼠

特异性

识别小鼠NLRP3/ NALP3

识别人,小鼠的NLRP3/ NALP3

纯度

≥95% (SDS-PAGE)

浓度详情

Protein G-affinity purified.

浓度

1 mg/mL

配方

液体、含10%甘油的PBS和0.02%的叠氮化钠。

产品类型

单克隆抗体

运输和装卸

运输

BLUE ICE

短期储存温度

+4°C

长期储存温度

-20°C

建议处置方式

开封后, 分装并储存在-20°C的地方,避免冷冻/解冻循环。

使用/稳定性

收货后保存在-20°C的地方,可稳定保存至少一年。

案例应用

抗小鼠NLRP3/NALP3单抗(Cryo-1)

NLRP3抗体                              anti-NLRP3/NALP3, mAb

 

  使用抗小鼠NLRP3/NALP3单抗(Cryo-1)检测小鼠巨噬细胞中NLRP3的含量。
  细胞提取物来源于小鼠巨噬细胞WT(泳道1)或NLRP3 KO(泳道2), 在还原条件下用SDS-PAGE 分离, 转移到硝酸纤维素膜后,用抗小鼠NLRP3/NALP3单抗(Cryo-1) (1 μg/mL) 孵育并检测。在化学发光检测系统中,蛋白质是可视化的。

 

NLRP3/NALP3单抗(Cryo-2)

NLRP3抗体                              anti-NLRP3/NALP3, mAb

  用anti-NLRP3/NALP3, mAb (Cryo-2)分别检测人和小鼠的THP1 细胞及巨噬细胞中的NLRP3/NALP3 含量。

A)细胞提取物来自于人THP1 细胞(泳道1)或THP1 表达的shRNA hNLRP3(泳道2)

B)细胞提取物来自于小鼠巨噬细胞 WT(泳道1)或NLRP3/NALP3 KO(泳道2),用SDS-PAGE 电泳分离,转移到硝酸纤维素膜后,

     用anti-NLRP3/NALP3,mAb (Cryo-2) (1 μg/mL) 孵育,并检测。在化学发光检测系统中,蛋白质是可视化的。

NLRP3抗体                              anti-NLRP3/NALP3, mAb

使用NLRP3(Cryo-2)单抗(产品编号AG-20B-0014)在小鼠巨噬细胞中检测小鼠NLRP3。

实验方法:从小鼠巨噬细胞(BMDM)WT +/+(lane 1),NLRP3 +/-(lane 2)或NLRP3 -/- (lane 3)的细胞提取物,有或没

                 有用LPS(50n g/mL)处理3 h,在还原条件下通过SDS-PAGE分离,转移至硝酸纤维素膜并与NLRP3(Cryo-2)的

                 mAb(1 μg/mL)共同孵育。 蛋白通过化学发光显影。图片由德国慕尼黑大学的Dr. Olaf Gross提供。

产品列表

产品编号

产品名称

规格

AG-20B-0006-C100

anti-NLRP3/NALP3 (mouse), mAb (Cryo-1)
抗小鼠NLRP3/NALP3单抗(Cryo-1)

100 μg

AG-20B-0014-C100

anti-NLRP3/NALP3, mAb (Cryo-2)
NLRP3/NALP3单抗(Cryo-2)

100 μg

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AG NLRP3 Inflammasome

AG免疫&炎症研究


  NLRP3/NALP3单抗(Cryo-2)的参考文献

1.

Thioredoxin-interacting protein links oxidative stress to inflammasome activation: R. Zhou, et al.; Nature Immunology 11, 136 (2010)


2.

The NLRP3 inflammasome promotes renal inflammation and contributes to CKD: A. Vilaysane, et al.; J. Am. Soc. Nephrol. 21, 1732 (2010)


3.

A role for mitochondria in NLRP3 inflammasome activation: R. Zhou, et al.; Nature 469, 221 (2011)


4.

Differential expression of NLRP3 among hematopoietic cells: G. Guarda, et al.; J. Immunol. 186, 2529 (2011)


5.

Mechanism of Impaired NLRP3 Inflammasome Priming by Monophosphoryl Lipid A: C.A. Embry, et al.; Sci. Signal. 4, ra28 (2011)


6.

Measles Virus V Protein Inhibits NLRP3 Inflammasome-Mediated Interleukin-1 Secretion: N. Komune, et al.; J. Virol. 85, 13019 (2011)


7.

Novel role of PKR in inflammasome activation and HMGB1 release: B. Lu, et al.; Nature 488, 670 (2012)


8.

Deubiquitination of NLRP3 by BRCC3 Critically Regulates Inflammasome Activity: B.F. Py, et al.; Mol. Cell 49,331 (2012)


9.

NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice: M.T. Heneka, et al.; Nature 493, 67 (2012)


10.

Activation of autophagy by inflammatory signals limits IL-1b production by targeting ubiquitinated inflammasomes for destruction: C.-S. Shi, et al.; Nat. Immunol. 13, 255 (2012)


11.

Inflammasome-independent NLRP3 augments TGF-β signaling in kidney epithelium: W. Wang, et al.; J. Immunol. 190, 1239 (2013)


12.

Omega-3 Fatty Acids Prevent Inflammation and Metabolic Disorder through Inhibition of NLRP3 Inflammasome Activation: Y. Yan, et al.; Immunity 38, 1154 (2013)


13.

LRRFIP2 negatively regulates NLRP3 inflammasome activation in macrophages by promoting Flightless-I-mediated caspase-1 inhibition: J. Jin, et al.; Nat. Commun. 4, 2075 (2013)


14.

Proapoptotic Chemotherapeutic Drugs Induce Noncanonical Processing and Release of IL-1β via Caspase-8 in Dendritic Cells: C. Antonopoulus, et al. J. Immunol. 191, 4789 (2013)


15.

Constitutively activated NLRP3 inflammasome causes inflammation and abnormal skeletal development in mice: S.L. Bonar, et al.; PLoS One 7, e35979 (2012)


16.

FADD and caspase-8 mediate priming and activation of the canonical and noncanonical Nlrp3 inflammasomes: P. Gurung, et al.; J. Immunol. 192, 1835 (2014)


17.

XIAP Restricts TNF- and RIP3-Dependent Cell Death and Inflammasome Activation: M. Yabal, et al.; Cell Rep.7, 1796 (2014)


18.

The adaptor ASC has extracellular and 'prionoid' activities that propagate inflammation: B.S. Franklin, et al.; Nat. Immunol. 15, 727 (2014)


19.

The NLRP3 inflammasome is released as a particulate danger signal that amplifies the inflammatory response: A. Baroja-Mazo, et al.; Nat. Immunol. 15, 738 (2014)


20.

Toward a chemical marker for inflammatory disease: a fluorescent probe for membrane-localized thioredoxin: M.H. Lee, et al.; JACS 136, 8430 (2014)


21.

Prostaglandin E2 inhibits NLRP3 inflammasome activation through EP4 receptor and intracellular cyclic AMP in human macrophages: M. Sokolowska, et al.; J. Immunol. 194, 5472 (2015)


22.

The receptor NLRP3 is a transcriptional regulator of TH2 differentiation: M. Bruchard, et al.; Nat. Immunol. 16,859 (2015)


23.

NLRP3 Inflammasome Is Expressed and Functional in Mouse Brain Microglia but Not in Astrocytes: A. Gustin, et al.; PLos One 10, e0130624 (2015)


24.

Nlrp6 promotes recovery after peripheral nerve injury independently of inflammasomes: E. Ydens, et al.; J. Neuroinfl. 12, 143 (2015)


25.

The Crohn’s disease risk factor IRGM limits NLRP3 inflammasome activation by impeding its assembly and by mediating its selective autophagy: S. Mehto, et al.; Mol. Cell 73, 429 (2019)


26.

Lack of Protein Prenylation Promotes Nlrp3 Inflammasome Assembly in Human Monocytes: O.P. Skinner, et al.; J. Allergy Clin. Immunol. (Epub ahead of print) (2019)

产品编号 产品名称 产品规格 产品等级
AG-20B-0006-C100 anti-NLRP3/NALP3 (mouse), mAb (Cryo-1) 
抗小鼠NLRP3/NALP3单抗(Cryo-1)
100 μg
AG-20B-0014-C100 anti-NLRP3/NALP3, mAb (Cryo-2) 
NLRP3/NALP3单抗(Cryo-2)
100 μg