荧光团

  兼容的EFLUXX-ID^® 试剂

  Cy 3 & Cy 5的偶联物、Pl、7-AAD、APC、Texas Red、

  RFP、YFP、TAMRA、UV和Violet 染料

  EFLUXX-ID^® 绿色荧光染料

  GFP/eGFP、RFP、PI、7-AAD、APC、Cy 5的偶联物、

  Rhodamine   123、Texas Red、UV和Violet   染料

  EFLUXX-ID^® 金色荧光染料

  应用

  流式细胞术，荧光显微镜

  应用说明

  EFLUXX-ID^® 多药耐药性检测试剂盒可对活细胞（悬浮细胞和贴壁细胞）中的耐药表型进行检测和分析。

  品质保证

  每批次EFLUXX-ID^® Gold耐药检测试剂盒的样品试剂盒均已按照操作手册中的步骤，对第5代和第20代间的

  CHO K1细胞系进行染色。获得的结果如下：

  1.   维拉帕米介导的抑制后MAF>80

  2. MK-571介导的抑制后MAF>60

  3. 新生霉素介导的抑制后MAF>40

  包装

  100 assays（流式细胞术）

  使用/稳定性

  自交货后，试剂盒组分在适当的储存条件下可稳定保存一年。

  储存条件

  避光。避免反复冻融。

  运输条件

  蓝冰运输

  短期储存

  -20°C

  长期储存

  -80°C

  组分

  2 瓶，EFLUXX-ID^® Gold或EFLUXX-ID^® Green检测试剂

  300 nmoles，MDR1 抑制剂（维拉帕米）

  750 nmoles，MRP   抑制剂（MK-571）

  1.5 µmoles，BCRP 抑制剂（新生霉素）

  500 µL，碘化丙啶

  科学背景

  耐药性是一种由跨膜ATP结合盒（ATP   Binding Cassette   ，简称ABC）转运蛋白家族上调介导的现象。

  ABC转运蛋白的过表达加速了毒性剂从细胞中去除，例如化疗剂从肿瘤细胞中流出，或抗生素从耐药菌株中流出。

  监管状态

  RUO – 仅供研究用

1. 

Exploiting off-target effects of estrogen deprivation to sensitize estrogen receptor negative breast cancer to immune killing: B. Wolfson, et al.; J. Immunother. Cancer 9, 2258 (2021), 摘要;

 2.

MicroRNA-324-5p regulates stemness, pathogenesis and sensitivity to bortezomib in multiple myeloma cells by targeting hedgehog signaling: B. Tang, et al.; Int. J. Cancer 142, 109 (2018), Application(s): Flow Cytometry; multiple myeloma, 摘要; Full Text

 3.

Upregulation of FOXM1 in a subset of relapsed myeloma results in poor outcome: C. Gu, et al.; Blood Cancer J. 8, 22 (2018), Application(s): Flow Cytometry; myeloma cells, 摘要; Full Text

 4.

Resistin induces multidrug resistance in myeloma by inhibiting cell death and upregulating ABC transporter expression: J. Pang, et al.; Haematologica 102, 1273 (2017), Application(s): Flow Cytometry; myeloma cells, 摘要; Full Text

 5.

Hepatocyte SLAMF3 reduced specifically the multidrugs resistance protein MRP-1 and increases HCC cells sensitization to anti-cancer drugs: G. Fouquet, et al.; Oncotarget 7, 32493 (2016), 摘要; Full Text

 6.

Intercellular transfer of P-glycoprotein in human blood-brain barrier endothelial cells is increased by histone deacetylase inhibitors: A. Noack, et al.; Sci. Rep. 6, 29253 (2016), Application(s): Flow cytometry of Pgp-EGFP transfer in co-cultured cells, 摘要; Full Text

 7.

The influence of a caveolin-1 mutant on the function of P-glycoprotein: C.Y. Lee, et al.; Sci. Rep. 6, 20486 (2016), 摘要; Full Text

 8.

RhoGDI2 up-regulates P-glycoprotein expression via Rac1 in gastric cancer cells: Z. Zheng, et al.; Cancer Cell Int. 15, 41 (2015), Application(s): Monitoring of MDR1 functionality by flow cytometry, 摘要; Full Text

 9.

Role of NEK2A in human cancer and its therapeutic potentials: J. Xia, et al.; Biomed. Res. Int. 2015, 862461 (2015), 摘要; Full Text

10.

Sonic hedgehog-glioma associated oncogene homolog 1 signaling enhances drug resistance in CD44+/Musashi-1+ gastric cancer stem cells: M. Xu, et al.; Cancer Lett. 369, 124 (2015), 摘要;

11.

Drug-induced trafficking of p-glycoprotein in human brain capillary endothelial cells as demonstrated by exposure to mitomycin C: A. Noack, et al.; PLoS One 9, e88154 (2014), Application(s): MDR status by flow cytometry in microvascular endothelial cells, 摘要; 全文

12.

NEK2 induces drug resistance mainly through activation of efflux drug pumps and is associated with poor prognosis in myeloma and other cancers: W. Zhou, et al.; Cancer Cell 23, 48 (2013), Application(s): Dye Efflux Assay for Multidrug Resistance was performed with ARP1 cancer cell lines and MCF7 cells using Gold detection reagent on flow cytometry, 摘要;

13.

Localization microscopy (SPDM) reveals clustered formations of P-glycoprotein in a human blood-brain barrier model: O. Huber, et al.; PLoS One 7, e44776 (2012), Application(s): MDR status by flow cytometry in immortalized human brain endothelial cells, 摘要; 全文

14.

Sensitive and specific fluorescent probes for functional analysis of the three major types of mammalian ABC transporters.: I. Lebedeva, et al.; PLoS One 6, e22429 (2011), 摘要; 全文

 1. 

P-Glycoprotein-mediated resistance to Hsp90-directed therapy is eclipsed by the heat shock response: A.K. McCollum, et al.; Cancer Res. 68, 7419 (2008), 摘要;

 2.

Glutathione export during apoptosis requires functional multidrug resistance-associated proteins: C.L. Hammond, et al.; J. Biol. Chem. 282, 14337 (2007), 摘要;

 3.

From MDR to MXR: new understanding of multidrug resistance systems, their properties and clinical significance: T. Litman, et al.; Cell Mol. Life Sci. 58, 931 (2001), 摘要;

 4.

Increased levels of the multidrug resistance protein in lateral membranes of proliferating hepatocyte-derived cells: H. Roelofsen, et al.; Gastroenterology 112, 511 (1997), 摘要;

 5.

How to probe clinical tumour samples for P-glycoprotein and multidrug resistance-associated protein: H.J. Broxterman, et al.; Eur. J. Cancer 32A, 1024 (1996), 摘要;

 6.

Methods to detect P-glycoprotein-associated multidrug resistance in patients’ tumors: consensus recommendations: W.T. Beck, et al.; Cancer Res. 56, 3010 (1996), 摘要;

 7.

Functional detection of MDR1/P170 and MRP/P190-mediated multidrug resistance in tumour cells by flow cytometry: N. Feller, et al.; Br. J. Cancer 72, 543 (1995), 摘要;

 8.

Immunocytochemical observation of multidrug resistance (MDR) p170 glycoprotein expression in human osteosarcoma cells. The clinical significance of MDR protein overexpression: B. Bodey, et al.; Anticancer Res. 15 (6B), 2461 (1995), 摘要;

 9.

Fluorescent cellular indicators are extruded by the multidrug resistance protein: L. Homolya, et al.; J. Biol. Chem. 268, 21493 (1993), 摘要;

10.

Polarized efflux of 2’,7’-bis(2-carboxyethyl)-5(6)-carboxyfluorescein from cultured epithelial cell monolayers: G.K. Collington, et al.; Biochem. Pharmacol. 44, 417 (1992), 摘要;

11.

Epithelial secretion of vinblastine by human intestinal adenocarcinoma cell (HCT-8 and T84) layers expressing P-glycoprotein: J. Hunter, et al.; Br. J. Cancer 64, 437 (1991), 摘要;

12.

Intrinsic multidrug resistance phenotype of Chinese hamster (rodent) cells in comparison to human cells: R.S. Gupta; BBRC 153, 598 (1988), 摘要;