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Cy3 酪胺
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货号 | 11065 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 1 mg | 价格 | 1236 | |
| Ex (nm) | 555 | Em (nm) | 569 | |
| 分子量 | 863.96 | 溶剂 | DMSO | |
| 产品详细介绍 | ||||
简要概述
产品基本信息
货号：11065
产品名称：Cy3 酪胺
规格：1mg
储存条件：-15℃避光防潮
保质期：12个月
产品物理化学光谱特性
分子量：863.96
外观：固体
溶剂：DMSO
激发波长(nm)：555
发射波长(nm)：565
适用仪器
| 荧光显微镜 | |
| 激发： | Cy3/TRITC滤波片组 |
| 发射： | Cy3/TRITC滤波片组 |
| 推荐孔板： | 黑色透明 |
产品介绍
Cy3 酪胺是美国AAT Bioquest生产的花菁染料，对于许多免疫组织化学（IHC）应用，传统的酶促扩增程序足以实现足够的抗原检测。但是，有几个因素限制了这些程序的敏感性和实用性。事实证明，酪胺信号放大（TSA）是一种特别通用且功能强大的酶扩增技术，具有更高的检测灵敏度。 TSA基于HRP在低浓度过氧化氢存在下将含有标记酪胺的底物转化为氧化的高反应性自由基的能力，该自由基可与HRP处或附近的酪氨酸残基共价结合。为了实现最大的IHC检测，酪胺用荧光团预标记。每个过氧化物酶标记物的多个酪胺底物的转换所赋予的信号放大转化了对低丰度靶标的超灵敏检测以及使用较少量的抗体和杂交探针。在免疫组织化学应用中，源自TSA方法的灵敏度增强允许增加一抗稀释以减少非特异性背景信号，并且可以克服由次优固定程序或低水平靶表达引起的弱免疫标记。 Cy3酪胺含有明亮的Cy3，可以使用标准Cy3过滤器组轻松检测。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Cy3 酪胺检测试剂。
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参考文献
Enhanced detection of Porcine reproductive and respiratory syndrome virus in fixed tissues by in situ hybridization following tyramide signal amplification
Authors: Trang NT, Hirai T, Ngan PH, Lan NT, Fuke N, Toyama K, Yamamoto T, Yamaguchi R.
Journal: J Vet Diagn Invest (2015): 326
Tyramide Signal Amplification for Immunofluorescent Enhancement
Authors: Faget L, Hnasko TS.
Journal: Methods Mol Biol (2015): 161
KSHV cell attachment sites revealed by ultra sensitive tyramide signal amplification (TSA) localize to membrane microdomains that are up-regulated on mitotic cells
Authors: Garrigues HJ, Rubinchikova YE, Rose TM.
Journal: Virology (2014): 75
Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis
Authors: Stack EC, Wang C, Roman KA, Hoyt CC.
Journal: Methods (2014): 46
Rapid and sensitive detection of Escherichia coli O157:H7 in milk and ground beef using magnetic bead-based immunoassay coupled with tyramide signal amplification
Authors: Aydin M, Herzig GP, Jeong KC, Dunigan S, Shah P, Ahn S.
Journal: J Food Prot (2014): 100
Sensitive whole-mount fluorescent in situ hybridization in zebrafish using enhanced tyramide signal amplification
Authors: Lauter G, Soll I, Hauptmann G.
Journal: Methods Mol Biol (2014): 175
Characterization of GABAergic neurons in the mouse lateral septum: a double fluorescence in situ hybridization and immunohistochemical study using tyramide signal amplification
Authors: Zhao C, Eisinger B, Gammie SC.
Journal: PLoS One (2013): e73750
Quantification of alpha-tubulin isotypes by sandwich ELISA with signal amplification through biotinyl-tyramide or immuno-PCR
Authors: Draberova E, Stegurova L, Sulimenko V, Hajkova Z, Draber P.
Journal: J Immunol Methods (2013): 63
Development of a near-infrared fluorescence ELISA method using tyramide signal amplification
Authors: Gong H, Cradduck M, Cheung L, Olive DM.
Journal: Anal Biochem (2012): 27
Integrated tyramide and polymerization-assisted signal amplification for a highly-sensitive immunoassay
Authors: Yuan L, Xu L, Liu S.
Journal: Anal Chem (2012): 10737