上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
胰凝乳蛋白酶荧光底物BOC-Val-Pro-Arg-AMC
货号 | 13461 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 5 mg | 价格 | 1236 | |
Ex (nm) | 341 | Em (nm) | 441 | |
分子量 | 741.75 | 溶剂 | DMSO | |
产品详细介绍 |
简要概述
产品基本信息
货号:13461
产品名称:胰凝乳蛋白酶荧光底物BOC-Val-Pro-Arg-AMC
规格:5mg
储存条件:-15℃避光防潮
保质期:12个月
产品物理化学光谱特性
分子量:741.75
溶剂:DMSO
激发波长(nm):351
发射波长(nm):430
产品介绍
胰凝乳蛋白酶荧光底物BOC-Val-Pro-Arg-AMC是美国AAT Bioquest生产的荧光底物,BOC-Val-Pro-Arg-AMC是α-凝血酶的敏感荧光底物。非荧光基板产生亮蓝色荧光AMC产品,其具有Ex / Em = 351 / 430nm,并且可以使用DAPI滤光器组容易地检测。据报道,BOC-Val-Pro-Arg-AMC的α-凝血酶和α-凝血酶卟啉葡萄糖苷复合物的Km值分别为21和25μM。 BOC-Val-ProArg-AMC的α-凝血酶和α-凝血酶葡萄糖凝固酶复合物的Kcat值分别为105和89s-1。该底物也已用于MRSA(耐甲氧西林金黄色葡萄球菌)的快速检测。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的胰凝乳蛋白酶荧光底物BOC-Val-Pro-Arg-AMC。
点击查看光谱
参考文献
A fluorescence lifetime-based assay for protease inhibitor profiling on human kallikrein 7
Authors: Doering K, Meder G, Hinnenberger M, Woelcke J, Mayr LM, Hassiepen U.
Journal: J Biomol Screen (2009): 1
A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease
Authors: Bodenreider C, Beer D, Keller TH, Sonntag S, Wen D, Yap L, Yau YH, Shochat SG, Huang D, Zhou T, Caflisch A, Su XC, Ozawa K, Otting G, Vasudevan SG, Lescar J, Lim SP.
Journal: Anal Biochem (2009): 195
A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates
Authors: Nakata H, Ohtsuki T, Sisido M.
Journal: Anal Biochem (2009): 121
Dual-function probe to detect protease activity for fluorescence measurement and 19F MRI
Authors: Mizukami S, Takikawa R, Sugihara F, Shirakawa M, Kikuchi K.
Journal: Angew Chem Int Ed Engl (2009): 3641
Fluorescence resonance energy transfer-based assay for characterization of hepatitis C virus NS3-4A protease activity in live cells
Authors: Sabariegos R, Picazo F, Domingo B, Franco S, Martinez MA, Llopis J.
Journal: Antimicrob Agents Chemother (2009): 728
Improved detection of ovarian cancer metastases by intraoperative quantitative fluorescence protease imaging in a pre-clinical model
Authors: Sheth RA, Upadhyay R, Stangenberg L, Sheth R, Weissleder R, Mahmood U.
Journal: Gynecol Oncol (2009): 616
Label-free, homogeneous, and fluorescence “turn-on” detection of protease using conjugated polyelectrolytes
Authors: An L, Liu L, Wang S.
Journal: Biomacromolecules (2009): 454
Real-time detection of caspase-3-like protease activation in vivo using fluorescence resonance energy transfer during plant programmed cell death induced by ultraviolet C overexposure
Authors: Zhang L, Xu Q, Xing D, Gao C, Xiong H.
Journal: Plant Physiol (2009): 1773
Real-time monitoring of human enterovirus (HEV)-infected cells and anti-HEV 3C protease potency by fluorescence resonance energy transfer
Authors: Tsai MT, Cheng YH, Liu YN, Liao NC, Lu WW, Kung SH.
Journal: Antimicrob Agents Chemother (2009): 748
[Prokaryotic expression and Identification of dual-fluorescence fusion proteins of small ubiquitin-like modifier and sentrin-specific protease]
Authors: Wang X, Guo J, Wang J, Li S, Sun L, Lu J.
Journal: Sheng Wu Gong Cheng Xue Bao (2009): 701
相关产品
产品名称 | 货号 |
荧光底物BOC-Val-Pro-Arg-Aminoluciferin | Cat#13002 |