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死细胞核染料 DiTO -1 1 mM DMSO溶液 相当于TOTO -1 CAS 143413-84-7
货号 | 17575 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 0.2 ml | 价格 | 2544 | |
Ex (nm) | 514 | Em (nm) | 531 | |
分子量 | 1302.78 | 溶剂 | Water | |
产品详细介绍 |
简要概述
产品基本信息
货号:17575
产品名称:死细胞核染料 DiTO -1 1 mM DMSO溶液 相当于TOTO -1
CAS:143413-84-7
规格:0.2ml
储存条件:-15℃避光干燥
保质期:12个月
产品物理化学光谱特性
分子量:1302.78
溶剂:水
激发波长(nm):514
发射波长(nm):535
适用仪器
荧光显微镜 | |
激发: | FITC滤波片 |
发射: | FITC滤波片 |
推荐孔板: | 黑色透明 |
产品介绍
DiTO -1在化学上等同于TOTO®-1(TOTO®是Invitrogen的商标)。DiTO -1是一种碳花青二聚体,具有类似于FITC的绿色荧光。它不具有细胞渗透性,可以很容易地与Cy5和罗丹明区别开来,作为核复染和死细胞指示剂。在不存在核酸的情况下它是非荧光的,并且在与DNA结合后会产生非常明亮的荧光信号。DiTO -1可在培养细胞和石蜡切片中进行强而有选择性的核染色。通过细胞可渗透的Nuclear Red LCS1染料和不渗透细胞的TOTO®-1进行的同时标记可用于评估细胞活力。DiTO -1和Nuclear Red 的消光系数都比结合DNA的碘化丙啶大。
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参考文献
Chromatin staining of Drosophila testes
Authors: Bonaccorsi S, Giansanti MG, Cenci G, Gatti M.
Journal: Cold Spring Harb Protoc (2012)
In vivo cancer imaging by poly(ethylene glycol)-b-poly(varepsilon-caprolactone) micelles containing a near-infrared probe
Authors: Cho H, Indig GL, Weichert J, Shin HC, Kwon GS.
Journal: Nanomedicine (2012): 228
The fluorescent dyes TO-PRO-3 and TOTO-3 iodide allow detection of microbial cells in soil samples without interference from background fluorescence
Authors: Henneberger R, Birch D, Bergquist P, Walter M, Anitori RP.
Journal: Biotechniques (2011): 190
Effect of Mg ions on efficiency of gene electrotransfer and on cell electropermeabilization
Authors: Haberl S, Miklavcic D, Pavlin M.
Journal: Bioelectrochemistry (2010): 265
Visualizing nuclei in skin cryosections: viable options to 4’6-diamidino-2-phenylindol for confocal laser microscopy
Authors: Glaser K, Wilke K, Wepf R, Biel SS.
Journal: Skin Res Technol (2008): 324
Highly chlorinated Escherichia coli cannot be stained by propidium iodide
Authors: Phe MH, Dossot M, Guilloteau H, Block JC.
Journal: Can J Microbiol (2007): 664
DNA labeling in living cells
Authors: Martin RM, Leonhardt H, Cardoso MC.
Journal: Cytometry A (2005): 45
Chlorination effect on the fluorescence of nucleic acid staining dyes
Authors: Phe MH, Dossot M, Block JC.
Journal: Water Res (2004): 3729
Quinolinium, 1,1′-[1,3-propanediylbis[(dimethyliminio)-3,1-propanediyl]]bis[4-[3-(3-methyl-2(3 H)-benzothiazolylidene)-1-propen-1-yl]-,iodide (1:4)
Authors: Shan L.
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)
Selection of fluorescent probes for flow cytometric viability assessment of Listeria monocytogenes exposed to membrane-active and oxidizing disinfectants
Authors: Luppens SB, Barbaras B, Breeuwer P, Rombouts FM, Abee T.
Journal: J Food Prot (2003): 1393