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DAPI(4′,6-二脒基-2-苯基吲哚二盐酸盐)CAS 28718-90-3
货号 | 17510 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 10 mg | 价格 | 984 | |
Ex (nm) | 359 | Em (nm) | 457 | |
分子量 | 350.25 | 溶剂 | Water | |
产品详细介绍 |
简要概述
产品基本信息
货号:17510
产品名称:DAPI(4′,6-二脒基-2-苯基吲哚二盐酸盐)
CAS:28718-90-3
规格:10mg
储存条件:保存在冰箱-20℃干燥
保质期:6个月
产品物理化学光谱特性
分子量:350.25
溶剂:水
Ex(nm):358
Em(nm):461
产品介绍
DAPI 4,6-二脒基-2-苯基吲哚,二盐酸盐是美国AAT Bioquest生产的用于染色DNA的试剂,DAPI是一种与DNA结合产生强烈的荧光染色剂。它广泛用于荧光显微镜。由于DAPI可以通过完整的细胞膜,它可用于除固定细胞外的活细胞染色。对于荧光显微镜,DAPI被紫外光线激发。当与双链DNA结合时,其最大吸收在358nm,其发射最大值在461nm。DAPI也与RNA结合,但是它不像它与DNA结合时那样强烈地发荧光。 当与RNA结合时,其发射偏移至约500nm .DAPI的蓝色发射便于多重测定,因为DAPI与荧光素和绿色荧光蛋白(GFP)等绿色荧光分子或红色荧光染料之间的荧光重叠非常少。除了标记细胞核外,DAPI还用于检测细胞培养物中的支原体或病毒DNA。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的DNA染色试剂/试剂盒。
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参考文献
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Journal: Mucosal immunology (2019)
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β-tricalcium phosphate composite ceramics with high compressive strength, enhanced osteogenesis and inhibited osteoclastic activities
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δ-Tocopherol promotes thermogenic gene expression via PGC-1α upregulation in 3T3-L1 cells
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Journal: Biochemical and biophysical research communications (2018)
A Novel Heterozygous Missense Mutation in GNAT1 Leads to Autosomal Dominant Riggs Type of Congenital Stationary Night Blindness
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Acrylamide-induced oxidative stress and inflammatory response are alleviated by N-acetylcysteine in PC12 cells: Involvement of the crosstalk between Nrf2 and NF-$kappa$B pathways regulated by MAPKs
Authors: Xiaoqi Pan, Xu Wu, Dandan Yan, Cheng Peng, Chaolong Rao, Hong Yan
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Augmenter of liver regeneration promotes mitochondrial biogenesis in renal ischemia–reperfusion injury
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Glucocorticoid-activation system mediated glucocorticoid-insulin-like growth factor 1 (GC-IGF1) axis programming alteration of adrenal dysfunction induced by prenatal caffeine exposure
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In vivo tailor-made protein corona of prodrug-based nanoassembly fabricated by redox dual-sensitive paclitaxel prodrug for the superselective treatment of breast cancer
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Inactivation of the tight junction gene CLDN11 by aberrant hypermethylation modulates tubulins polymerization and promotes cell migration in nasopharyngeal carcinoma
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