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Cy5生物素缀合物
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货号 | 3100 | 存储条件 | |
| 规格 | 5 mg | 价格 | 2544 | |
| Ex (nm) | 651 | Em (nm) | 670 | |
| 分子量 | 1353.82 | 溶剂 | Water | |
| 产品详细介绍 | ||||
简要概述
产品基本信息
货号：3100
产品名称：Cy5生物素缀合物
规格：5mg
储存条件：-15℃避光防潮
保质期：12个月
产品物理化学光谱特性
分子量：1179.49
外观：固体
溶剂：水
激发波长（nm）：649
发射波长（nm）：665
适用仪器
| 荧光酶标仪 | |
| 激发： | 492nm |
| 发射： | 518nm |
| cutoff： | 500nm |
| 推荐孔板： | 黑色孔板 |
产品介绍
Cy5生物素缀合物是美国AAT Bioquest生产的Cy系列产品，这种双功能Cy5生物素结合物表现出优异的抗生物素蛋白结合和强荧光的特性。 就高亲和力、快速结合、与抗生物素蛋白和链霉抗生物素蛋白四聚体的非协同结合而言，其具有与天然生物素类似的抗生物素蛋白结合特性。 Cy5生物素可用于检测和定量抗生物素蛋白，链霉抗生物素蛋白或中性抗生物素蛋白的生物素结合位点。 该试剂克服了常用的生物素-4-荧光素的主要缺点，例如在水性介质中溶解性差和pH依赖性吸光度和发射。Cy5生物素是水溶性试剂，生物素和荧光标签之间的柔性PEG间隔物使与抗生物素蛋白、链霉抗生物素蛋白或中性抗生物素蛋白结合有关的空间位阻最小化。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Cy5生物素缀合物。
参考文献
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Authors: M. J. Waner
Journal: Biochem Biophys Rep (2019): 127-131
Biotin-tagged fluorescent sensor to visualize ‘mobile’ Zn(2+) in cancer cells
Authors: L. Fang
Journal: Chem Commun (Camb) (2018): 9619-9622
Synthesis of Two-Photon Active Tricomponent Fluorescent Probe for Distinguishment of Biotin Receptor Positive and Negative Cells and Imaging 3D-Spheroid
Authors: K. Pal
Journal: Org Lett (2018): 6425-6429
A green fluorescent protein-based assay for high-throughput ligand-binding studies of a mycobacterial biotin protein ligase
Authors: T. E. H. Bond
Journal: Microbiol Res (2017): 35-39
Determination of Biotin in Infant, Pediatric, and Adult Nutritionals by High-Performance Liquid Chromatography and Fluorescence Detection: Single-Laboratory Validation, First Action 2016.11
Authors: Q. Lin
Journal: J AOAC Int (2017): 145-151
Labeling of Cytoskeletal Proteins in Living Cells Using Biotin Ligase Carrying a Fluorescent Protein
Authors: S. Nishi
Journal: Anal Sci (2017): 897-902
A biotin-conjugated glutathione-responsive FRET-based fluorescent probe with a ferrocenyl BODIPY as the dark quencher
Authors: W. J. Shi
Journal: Dalton Trans (2016): 17798-17806
Design and synthesis of fluorescent and biotin tagged probes for the study of molecular actions of FAF1 inhibitor
Authors: S. E. Yoo
Journal: Bioorg Med Chem Lett (2016): 1169-72
Highly Selective Fluorescent Turn-On Probe for Protein Thiols in Biotin Receptor-Positive Cancer Cells
Authors: Q. Sun
Journal: Anal Chem (2016): 3400-5
NeutrAvidin Functionalization of CdSe/CdS Quantum Nanorods and Quantification of Biotin Binding Sites using Biotin-4-Fluorescein Fluorescence Quenching
Authors: L. G. Lippert
Journal: Bioconjug Chem (2016): 562-8