COVID-19荧光底物Z-KAGG-AMC

	货号	13552	存储条件	在零下15度以下保存, 避免光照
	规格	1 mg	价格	1176
	Ex (nm)	341	Em (nm)	441
	分子量	622.67	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：13552

产品名称：COVID-19荧光底物Z-KAGG-AMC

规格：1mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

外观：固体

溶剂：DMSO

激发波长(nm)：341

发射波长(nm)：441

产品介绍

冠状病毒（CoV）是一种可以感染人类和多种动物，因其形态看上去像中世纪欧洲帝王的皇冠，因此命名为“冠状病毒”。这类病毒具有胃肠道、呼吸道和神经系统的嗜性，可以导致多种疾病。在2019年底，一种新型的冠状病毒被称为严重急性呼吸系统综合症冠状病毒2（SARS-CoV-2），开始在全球蔓延。即使大多数受感染的患者仅患有轻度症状，例如发烧和咳嗽，但该疾病可能发展为致命的肺炎和急性呼吸衰竭病例，尤其是在多病体弱的的老人中。该病毒仅仅从出现开始的三四个月，全球已经感染了超过100万人。当前，尚无用于治疗Covid-19的任何具体有效的选择。目前，Covid-19的临床治疗主要是对症治疗，并结合上市的抗病毒药物（如瑞德昔韦和抗生素）以治疗继发感染。目前全世界都迫切需要开发抗SARS-CoV-2的特异性抗病毒治疗方法和疫苗，而冠状病毒主蛋白酶是抗CoV药物设计的重要靶点，在病毒基因表达和复制中起着关键作用。目前已掌握的治疗人类免疫缺陷病毒（HIV）和丙型肝炎的成功策略是抑制蛋白水解酶消灭病毒蛋白酶。而且证明了蛋白酶抑制剂治疗病毒感染具有非常大的潜力。同样，SARS-CoV-2病毒必须要进行复制的酶便是蛋白酶，因此Covid-19是抗病毒治疗极好的靶点。

冠状病毒是一种单链RNA正链包膜B型冠状病毒。像其他两种导致严重急性呼吸系统综合症（SARS）和中东呼吸系统综合症（MERS）的冠状病毒一样，SARS-CoV-2编码由非结构，结构和辅助蛋白组成。非结构蛋白包括3-胰凝乳蛋白酶样蛋白酶（3CLpro），木瓜蛋白酶，解旋酶和RNA依赖性RNA聚合酶（RNA依赖性RNA聚合酶（RdRp）），结构蛋白包括刺突糖蛋白。从大的多蛋白1ab上的11个切割位点开始，从病毒RNA翻译的多蛋白的加工是必不可少的，因此，主要的蛋白酶被认为是预防病毒模仿的诱人药物靶标。冠状病毒的木瓜蛋白酶样蛋白酶（PLpro）进行非结构蛋白的蛋白水解成熟，这些蛋白在病毒复制中起作用，并执行宿主细胞因子的去泛素化，从而破坏抗病毒反应。Z-KAGG-AMC被木瓜蛋白酶样蛋白酶裂解，得到高度荧光的AMC产物。释放的AMC的荧光强度与蛋白酶活性成正比。Z-KAGG-AMC可用于筛选和研究PLpro抑制剂的动力学。

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参考文献

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Analysis of Coronavirus Temperature-Sensitive Mutants Reveals an Interplay between the Macrodomain and Papain-Like Protease Impacting Replication and Pathogenesis.
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Journal: Phytopathology (2019): 736-747

First detection of novel enterovirus G recombining a torovirus papain-like protease gene associated with diarrhoea in swine in South Korea.
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Identification and design of novel small molecule inhibitors against MERS-CoV papain-like protease via high-throughput screening and molecular modeling.
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Porcine epidemic diarrhea virus papain-like protease 2 can be noncompetitively inhibited by 6-thioguanine.
Authors: Chu, Hsu-Feng and Chen, Chiao-Che and Moses, David C and Chen, Yau-Hung and Lin, Chao-Hsiung and Tsai, Ying-Chieh and Chou, Chi-Yuan
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The papain-like protease determines a virulence trait that varies among members of the SARS-coronavirus species.
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