Cell Meter 荧光法细胞电位检测试剂盒

	货号	35000	存储条件	Multiple
	规格	100 Tests	价格	8592
	Ex (nm)		Em (nm)
	分子量		溶剂
	产品详细介绍

简要概述

产品基本信息

货号：35000

产品名称：Cell Meter 荧光法细胞电位检测试剂盒

规格：100 Tests

保质期：12个月

产品介绍

几乎所有质膜在其上都具有电势，内部通常相对于外部为负。信号是通过打开或关闭膜上某一点的离子通道而产生的，从而在膜电位上产生局部变化。电场的这种变化会很快受到膜中相邻或较远的离子通道的影响。然后，由于电势变化，这些离子通道可以打开或关闭，从而产生信号。 Cell Meter 荧光细胞电位检测试剂盒使用FRET对（VSB 405和VSR 555）来检测细胞膜电位的变化。亲脂性VSB 405主要位于脂质膜的外层，而VSR 555的定位对细胞膜电位敏感。在静止状态下，的内层具有相对负的电势，从而使VSR 555主要位于细胞膜的外层附近，因此靠近蓝色荧光VSB405，从而导致从蓝色（VSB 405）到蓝色荧光的有效转移。红色（VSR 555）。当细胞去极化时，VSR 555易位到细胞膜的内层，从而分离FRET对并破坏FRET。蓝色/红色荧光比与细胞电位成正比。该检测法可用于筛选调节离子通道的化合物。

图示

图1.使用Cell Meter 荧光细胞电位检测试剂盒检测HeLa细胞中的膜电位。根据试剂盒说明对HeLa细胞进行染色，并用去极化溶液（164.5 mM KCl，2 mM CaCl2、1 mM MgCl2、10 mM葡萄糖，20 mM HEPES，pH 7.4）刺激。使用FlexStation 3（分子设备）记录响应。

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