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Cy5-dATP
货号 | 17038 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 25 nmoles | 价格 | 1176 | |
Ex (nm) | 651 | Em (nm) | 670 | |
分子量 | 1182.05 | 溶剂 | Water | |
产品详细介绍 |
简要概述
产品基本信息
货号:17038
产品名称:Cy5-dATP
规格:25 nmoles
储存条件:-15℃避光防潮
保质期:24个月
产品物理化学光谱特性
分子量:1182.05
溶剂:水
激发波长(nm):651
发射波长(nm):670
消光系数(cm-1 M-1):250000
荧光量子产率:0.27(PBS),0.4(乙醇)
校正因子 (280 nm):0.03
产品介绍
染料修饰的脱氧腺苷5′-三磷酸酯广泛用于各种非放射性DNA标记反应,包括切口平移,随机引物标记,cDNA标记和3′-末端标记。 Cy5-dATP可用于通过常规酶促掺入方法(例如逆转录,缺口翻译,随机引物标记或PCR)产生含Cy5的DNA。这种酶促荧光标记方法广泛用于FISH探针和基于微阵列的实验。此Cy5-dATP缀合物可与SpectrumFRed 滤波片组一起用作红色荧光色(SpectrumFRed 是Vysis的商标)。它在功能上等同于Cy5-dATP(珀金·埃尔默(Perkin Elmer)的NEL593001EA),稳定性大大提高。
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参考文献
2-Formyl-dATP as Substrate for Polymerase Synthesis of Reactive DNA Bearing an Aldehyde Group in the Minor Groove.
Authors: Krömer, Matouš and Brunderová, Mária and Ivancová, Ivana and Poštová Slavětínská, Lenka and Hocek, Michal
Journal: ChemPlusChem (2020): 1164-1170
Fluorescent dATP for DNA Synthesis In Vivo.
Authors: Schreier, Verena N and Loehr, Morten O and Deng, Ting and Lattmann, Evelyn and Hajnal, Alex and Neuhauss, Stephan C F and Luedtke, Nathan W
Journal: ACS chemical biology (2020): 2996-3003
New bioluminescence pyrophosphate assay for high-sensitivity detection of food-borne pathogens.
Authors: Fei, Zhongjie and Zhou, Dongrui and Li, Na and Xiao, Pengfeng
Journal: Luminescence : the journal of biological and chemical luminescence (2020): 355-364
Binding studies of a putative C. pseudotuberculosis target protein from Vitamin B12 Metabolism.
Authors: Peinado, Rafaela Dos S and Olivier, Danilo S and Eberle, Raphael J and de Moraes, Fabio R and Amaral, Marcos S and Arni, Raghuvir K and Coronado, Monika A
Journal: Scientific reports (2019): 6350
ELTA: Enzymatic Labeling of Terminal ADP-Ribose.
Authors: Ando, Yoshinari and Elkayam, Elad and McPherson, Robert Lyle and Dasovich, Morgan and Cheng, Shang-Jung and Voorneveld, Jim and Filippov, Dmitri V and Ong, Shao-En and Joshua-Tor, Leemor and Leung, Anthony K L
Journal: Molecular cell (2019): 845-856.e5
Label-Free Telomerase Detection in Single Cell Using a Five-Base Telomerase Product-Triggered Exponential Rolling Circle Amplification Strategy.
Authors: Li, XiaoYu and Cui, YunXi and Du, YiChen and Tang, AnNa and Kong, DeMing
Journal: ACS sensors (2019): 1090-1096
Terminal deoxynucleotidyl transferase-initiated molecule beacons arrayed aptamer probe for sensitive detection of metastatic colorectal cancer cells.
Authors: Zhao, Yujie and Ma, Wenjie and Zou, Shanzi and Chen, Biao and Cheng, Hong and He, Xiaoxiao and Wang, Kemin
Journal: Talanta (2019): 152-158
2-Allyl- and Propargylamino-dATPs for Site-Specific Enzymatic Introduction of a Single Modification in the Minor Groove of DNA.
Authors: Matyašovský, Ján and Pohl, Radek and Hocek, Michal
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2018): 14938-14941
DNA Primer Extension with Cyclopropenylated 7-Deaza-2′-deoxyadenosine and Efficient Bioorthogonal Labeling in Vitro and in Living Cells.
Authors: Ploschik, Damian and Rönicke, Franziska and Beike, Hanna and Strasser, Ralf and Wagenknecht, Hans-Achim
Journal: Chembiochem : a European journal of chemical biology (2018): 1949-1953
Gp41, a superfamily SF2 helicase from bacteriophage BFK20.
Authors: Halgasova, Nora and Matuskova, Radka and Kraus, Daniel and Tkacova, Adela and Balusikova, Lenka and Bukovska, Gabriela
Journal: Virus research (2018): 7-16