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ReadiView 绿色/红色 细胞活性染料
货号 | 22763 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 1000 Tests | 价格 | 5940 | |
Ex (nm) | Em (nm) | |||
分子量 | 溶剂 | Water | ||
产品详细介绍 |
简要概述
ReadiView 绿色/红色活性染料是一种耐用的细胞染色剂,经过优化,可用于对Countess II FL或Countess 3 FL仪器中的有核细胞进行染色。 它可以方便地与传统的FITC或GFP滤光片组配合使用,例如EVOS GFP荧光灯适用于激发和检测绿色染料,而RFP或Texas Red(TxR)荧光灯适用于细胞红染色。 绿色表示活细胞,而红色表示死细胞。 ReadiView染料可对混合群体中的有核细胞进行特定染色,从而降低了复杂性,并在与适当的EVOS光学立方体匹配时提高了计数与样品间的可重复性。
产品说明书
样本实验方案
将10 µL ReadyCount 活/死活力染色剂与10 µL细胞混合。
在室温下孵育2至5分钟。
将10 µL染色的细胞样品上样至Countess细胞计数仪的玻片中。
在Countess细胞计数仪上读取数据。
图示
图1.用ReadyView 绿色/红色细胞活性染料标记的Jurkat细胞的荧光图像。
参考文献
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