iFluor 488-dUTP * 1 mM的Tris缓冲液(pH 7.5)* 货号17039-AAT Bioquest荧光染料

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iFluor 488-dUTP * 1 mM的Tris缓冲液(pH 7.5)*

iFluor 488-dUTP * 1 mM的Tris缓冲液(pH 7.5)*

iFluor 488-dUTP * 1 mM的Tris缓冲液(pH 7.5)*    货号17039 货号 17039 存储条件 在零下15度以下保存, 避免光照
规格 25 nmoles 价格 1140
Ex (nm) 491 Em (nm) 516
分子量 1152.83 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17039

产品名称:iFluor 488-dUTP

规格:25 nmoles

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:1152.83

溶剂:水

激发波长(nm):491

发射波长(nm):516

 

产品介绍

染料修饰的脱氧尿苷5′-三磷酸酯是通过常规酶掺入方法(例如反转录,缺口翻译,随机引物标记或PCR)生产染料标记DNA的最常用方法之一。 这种酶促荧光标记方法广泛用于FISH探针和基于微阵列的实验。 该iFluor 488-dUTP缀合物与SpectrumGreen 滤光片组合一起用作绿色荧光(SpectrumGreen 是Vysis的商标)。 与常用的绿色探针(例如Fluorescein-12-dUTP)相比,iFluor 488提供了更明亮的信号,具有很高的光稳定性,并且不受pH值的影响。

点击查看光谱

 

参考文献

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Flow cytometric detection of beta-D-glucuronidase gene in wild-type bacterial cells using in-situ PCR.
Authors: Sachidanandham, Ramaiah and Gin, Karina Yew-Hoong
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Lack of nuclear apoptosis in cardiomyocytes and increased endothelin-1 levels in a rat heart model of myocardial stunning.
Authors: Klainguti, M and Aigner, S and Kilo, J and Eppenberger, H M and Mandinova, A and Aebi, U and Schaub, M C and Shaw, S G and Lüscher, T F and Atar, D
Journal: Basic research in cardiology (2000): 308-15

A method to monitor DNA transfer during transfection.
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Journal: AAPS pharmSci (1999): E6

Pulsatile shear stress leads to DNA fragmentation in human SH-SY5Y neuroblastoma cell line.
Authors: Triyoso, D H and Good, T A
Journal: The Journal of physiology (1999): 355-65

Detection of TNF alpha and Fas ligand mRNA within synovial mononuclear cells by fluorescence in-cell labeling PCR (FICL-PCR).
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Targeted nucleotide exchange in the alkaline phosphatase gene of HuH-7 cells mediated by a chimeric RNA/DNA oligonucleotide.
Authors: Kren, B T and Cole-Strauss, A and Kmiec, E B and Steer, C J
Journal: Hepatology (Baltimore, Md.) (1997): 1462-8

说明书
iFluor 488-dUTP * 1 mM的Tris缓冲液(pH 7.5)*.pdf