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噻唑橙 CAS 107091-89-4
货号 | 17518 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 100 mg | 价格 | 2544 | |
Ex (nm) | 514 | Em (nm) | 533 | |
分子量 | 476.61 | 溶剂 | DMSO | |
产品详细介绍 |
简要概述
产品基本信息
货号:17518
产品名称:噻唑橙
CAS:107091-89-4
规格:100mg
储存条件:保存在冰箱-20℃干燥
保质期:12个月
产品物理化学光谱特性
分子量:476.61
外观:红色
溶剂:DMSO
Ex(nm):512
Em(nm):533
产品介绍
噻唑橙是用于网织红细胞分析的荧光DNA结合染料。它对疟原虫物种分析也很有用。改性噻唑橙衍生物(如SYBR Green,PicoGreen,TOTO和YOYO)广泛用于检测DNA样品。
点击查看光谱
产品说明书
染色细胞示例
以下程序适用于大多数细胞类型。生长培养基,细胞密度,其他细胞类型的存在和其他因素可能影响染色。玻璃器皿上的残留洗涤剂也可能影响许多生物的真实或明显染色,导致在有或没有细胞存在的溶液中出现明亮染色的物质。
1)制备1-10mM DMSO储备溶液。如果储存在-20°C,DMSO储备液可以保存6个月。
2)使用适合您样品的固定方案。
3)通过离心沉淀细胞,并将细胞重悬于缓冲盐溶液或培养基中,在pH 7.4下进行最佳染料染色。培养物中的贴壁细胞可以在盖玻片上或细胞培养孔中原位染色。
4)使用0.5和10μM之间的浓度添加噻唑橙染色并孵育15至60分钟作为指导。
注意:在最初的实验中,最好在整个建议范围内尝试几种染料浓度确定产生最佳染色的浓度。
参考文献
Effect of UVC-Induced Photooxidation of DNA on the Intercalation of Thiazole Orange: A Convenient Reporter for Oxidative Damage
Authors: Trevithick-Sutton CC, Mikelsons L, Filippenko V, Scaiano JC.
Journal: Photochem Photobiol. (2006)
Exploring Base-Pair-Specific Optical Properties of the DNA Stain Thiazole Orange
Authors: Jarikote DV, Krebs N, Tannert S, Roder B, Seitz O.
Journal: Chemistry. (2006)
Fluorescence resonance energy transfer and complex formation between thiazole orange and various dye-DNA conjugates: implications in signaling nucleic acid hybridization
Authors: Algar WR, Massey M, Krull UJ.
Journal: J Fluoresc (2006): 555
Large dynamic Stokes shift of DNA intercalation dye Thiazole Orange has contribution from a high-frequency mode
Authors: Karunakaran V, Perez Lustres JL, Zhao L, Ernsting NP, Seitz O.
Journal: J Am Chem Soc (2006): 2954
Thiazole orange, a DNA-binding photosensitizer with flexible structure, can inactivate pathogens in red blood cell suspensions while maintaining red cell storage properties
Authors: Skripchenko A, Wagner SJ, Thompson-Montgomery D, Awatefe H.
Journal: Transfusion (2006): 213
Forced intercalation probes (FIT Probes): thiazole orange as a fluorescent base in peptide nucleic acids for homogeneous single-nucleotide-polymorphism detection
Authors: Kohler O, Jarikote DV, Seitz O.
Journal: Chembiochem (2005): 69
Sequence-specific fluorescence detection of DNA by polyamide-thiazole orange conjugates
Authors: Fechter EJ, Olenyuk B, Dervan PB.
Journal: J Am Chem Soc (2005): 16685
Synthesis and fluorescence studies of thiazole orange tethered onto oligonucleotide: development of a self-contained DNA biosensor on a fiber optic surface
Authors: Wang X, Krull UJ.
Journal: Bioorg Med Chem Lett (2005): 1725
Double staining of Plasmodium falciparum nucleic acids with hydroethidine and thiazole orange for cell cycle stage analysis by flow cytometry
Authors: Jouin H, Daher W, Khalife J, Ricard I, Puijalon OM, Capron M, Dive D.
Journal: Cytometry A (2004): 34
Thiazole orange positive platelets in a dog with Evans’ syndrome
Authors: Michimoto T, Okamura T, Suzuki K, Watari T, Kano R, Hasegawa A.
Journal: J Vet Med Sci (2004): 1305
相关产品
产品名称 | 货号 |
噻唑橙*10mM DMSO溶液* | Cat#17519 |