钙离子荧光探针Rhod-4, AM 货号21120-AAT Bioquest荧光染料

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钙离子荧光探针Rhod-4, AM

钙离子荧光探针Rhod-4, AM

钙离子荧光探针Rhod-4, AM    货号21120 货号 21120 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 6432
Ex (nm) 523 Em (nm) 551
分子量 1015.96 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:21120

产品名称:钙离子荧光探针Rhod-4, AM

规格:1mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:1015.96

溶剂:DMSO

激发波长(nm):524

发射波长(nm):551

 

适用仪器


荧光显微镜  
激发: TRITC
发射: TRITC
推荐孔板: 黑色透明
荧光酶标仪  
激发: 540nm
发射: 590nm
cutoff: 570nm
推荐孔板: 黑色透明
读取模式: 底读模式

 

产品介绍

钙离子荧光探针Rhod-4, AM是美国AAT Bioquest生产的钙离子荧光探针,钙测量对于许多生物学研究至关重要。在结合Ca2+后显示光谱响应的荧光探针使研究人员能够使用荧光显微镜,流式细胞仪,荧光光谱和荧光酶标仪来研究细胞内游离Ca2+浓度的变化。在红色荧光钙指示剂中,Rhod-2最常用。但是,Rhod-2 AM在酯酶水解后仅在活细胞中呈中等荧光,并且细胞钙反应非常小。Rhod-4 的开发旨在改善Rhod-2细胞的负载和钙反应,同时保持Rhod-2的光谱波长。在CHO和HEK细胞中,Rhod-4 AM的细胞钙反应敏感性比Rhod-2 AM高10倍。AAT Bioquest提供Quest Rhod-4的多种包装大小,可满足您的特殊需求,例如1 mg;10×50 µg;20×50 µg;HTS包装,不收取额外包装费用。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的钙离子荧光探针Rhod-4, AM。 

点击查看光谱

点击查看实验方案

钙离子篇:时间轴式讲解应用于钙离子检测的探针

 

参考文献

A high-throughput Ca2+ flux assay to monitor cyclic nucleotide-gated channel activity and characterize achromatopsia mutant channel function
Authors: Marlene A Jacobson, Laura J Jones, Dennis J Colussi, Jacqueline C Tanaka
Journal: ACS chemical neuroscience (2019)

Central role of IP 3 R2-mediated Ca 2+ oscillation in self-renewal of liver cancer stem cells elucidated by high-signal ER sensor
Authors: Cuiwei Sun, Bo Shui, Wei Zhao, Hui Liu, Wenwen Li, Jane C Lee, Robert Doran, Frank K Lee, Tao Sun, Qing Sunny Shen
Journal: Cell death & disease (2019): 396

Imaging elemental events of store-operated Ca2+ entry in invading cancer cells with plasmalemmal targeted sensors
Authors: Fujian Lu, Jianwei Sun, Qiaoxia Zheng, Jinghang Li, Yuanzhao Hu, Peng Yu, Huifang He, Yan Zhao, Xianhua Wang, Shengyu Yang
Journal: J Cell Sci (2019): jcs–224923

Manipulating energy migration within single lanthanide activator for switchable upconversion emissions towards bidirectional photoactivation
Authors: Qingsong Mei, Akshaya Bansal, Muthu Kumara Gnanasammandhan Jayakumar, Zhiming Zhang, Jing Zhang, Hua Huang, Dejie Yu, Chrishan JA Ramachandra, Derek J Hausenloy, Tuck Wah Soong
Journal: Nature Communications (2019): 1–11

Autocrine GABA signaling distinctively regulates phenotypic activation of mouse pulmonary macrophages
Authors: Luan Januzi, Jacob W Poirier, Matthew JE Maksoud, Yun-Yan Xiang, Rudolf AW Veldhuizen, Sean E Gill, Sean P Cregan, Haibo Zhang, Gregory A Dekaban, Wei-Yang Lu
Journal: Cellular Immunology (2018)

Three-dimensional model of intracellular and intercellular Ca2+ waves propagation in endothelial cells
Authors: Toshihiro Sera, Shingo Komine, Masataka Arai, Yasuhiro Sunaga, Hideo Yokota, Susumu Kudo
Journal: Biochemical and Biophysical Research Communications (2018)

Effect of stem cell niche elasticity/ECM protein on the self-beating cardiomyocyte differentiation of inducedpluripotent stem (iPS) cells at different stages
Authors: Mitsuhi Hirata, Tetsuji Yamaoka
Journal: Acta Biomaterialia (2017)

Emerin plays a crucial role in nuclear invagination and in the nuclear calcium transient
Authors: Masaya Shimojima, Shinsuke Yuasa, Chikaaki Motoda, Gakuto Yozu, Toshihiro Nagai, Shogo Ito, Mark Lachmann, Shin Kashimura, Makoto Takei, Dai Kusumoto
Journal: Scientific Reports (2017)

Preliminary findings on ultrasound modulation of the electromechanical function of human stem-cell-derived cardiomyocytes
Authors: Andrew William Chen, Aleksandra Klimas, Vesna Zderic, Ivan Suares Castellanos, Emilia Entcheva
Journal: (2017): 1–4

The role of spatial organization of Ca (2+) release sites in the generation of arrhythmogenic diastolic Ca (2+) release in myocytes from failing hearts.
Authors: Andriy E Belevych, Hsiang-Ting Ho, Ingrid M Bonilla, Radmila Terentyeva, Karsten E Schober, Dmitry Terentyev, Cynthia A Carnes, Sándor Györke
Journal: Basic research in cardiology (2017): 44

说明书
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