上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
ReadiCleave iFluor 488 AML-NHS酯
货号 | 7005 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 1 mg | 价格 | 4740 | |
Ex (nm) | 491 | Em (nm) | 516 | |
分子量 | 1340.49 | 溶剂 | DMSO | |
产品详细介绍 |
简要概述
产品基本信息
货号:7005
产品名称:ReadiCleave iFluor 488 AML-NHS酯
规格:1mg
储存条件:-15℃避光防潮
保质期:12个月
产品物理化学光谱特性
分子量:1296.43
外观:固体
溶剂:DMSO
Ex:491nm
Em:516nm
产品介绍
荧光法生物检测在灵敏度和便利性的方面具有很大的优势。许多生物分子可以很容易地用荧光标签标记,用于荧光成像和流式细胞仪分析。 然而,大多数现有的荧光标签用于永久性标记生物靶标,从中不能切割添加的荧光标签以进行进一步的下游分析,例如质谱分析。 AAT Bioquest的ReadiCleave 探针使荧光标签与生物靶标结合,可以在需要时从中去除附加的荧光标签。 此ReadiCleave AML iFluor 488包含一个叠氮基甲基化合物,可以用TCEP裂解该化合物,以从靶分子中除去iFluor 488荧光团。 可以通过添加10 mM TCEP溶液(pH 7.5)并在65°C孵育1-5分钟来进行裂解。iFluor 488是AlexaFluor®488的卓越替代品。iFluor 488和AlexaFluor®488具有非常相似的光谱特性。
点击查看光谱
参考文献
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