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ReadiUse™10KD旋转柱
货号 | 60502 | 存储条件 | 室温储存不要冷冻 | |
规格 | 5 | 价格 | 936 | |
Ex (nm) | Em (nm) | |||
分子量 | 溶剂 | |||
产品详细介绍 |
简要概述
ReadiUse™10KD旋转柱是用于浓缩生物样品(如抗体)的一次性超滤设备。最大初始样品量为〜500 ul。它们既可以用在摆动式转子,也可以用在固定式转子中,接受2.0 g约10000 g的离心管。止动体积为〜20-25 µl。垂直的膜设计和细通道过滤室可最大程度地减少膜污染,并提供高速浓缩甚至满载的溶液。它经过专门设计,具有低内表面和膜面积,可从稀溶液中获得优异的回收率。
产品说明书
样品分析方案
详细参数
聚丙烯外壳和聚醚砜(PES)膜
MWCO:10K
样品量:25 µL-500 µL
样品回收率高
应用:蛋白质样品的浓缩,脱盐,缓冲液交换和脱蛋白
最大离心力:14,000克
推荐离心力:5,000〜10,000 g
操作步骤
将蛋白质样品加到过滤器中,以8,000〜10,000 g离心。
注意:从500 µL浓缩到〜50 µL大约需要10到15分钟。
脱盐,缓冲液交换或渗滤是去除含生物分子溶液中盐或溶剂的重要方法。可以通过以下方法在ReadiUse™10KD离心柱中去除盐或交换缓冲液:浓缩样品,然后用任何所需的溶剂将浓缩液重新配制为原始样品体积。可以重复“洗出”的过程,直到污染微溶质的浓度充分降低为止。
将细胞裂解物或组织匀浆加入滤膜,以10,000 g离心10到15min,收集洗脱液。
图1. ReadiUse™10KD旋转柱是用于浓缩生物样品(如抗体)的一次性超滤设备。最大初始样品量为〜500 ul。它们既可以用在摆动式转子中,也可以用在固定角转子中,接受2.0 g约10000 g的离心管。
参考文献
Extraction of β-blockers from urine with a polymeric monolith modified with 1-allyl-3-methylimidazolium chloride in spin column format.
Authors: Mompó-Roselló, Oscar and Ribera-Castelló, Ana and Simó-Alfonso, Ernesto F and Ruiz-Angel, María José and García-Alvarez-Coque, María Celia and Herrero-Martínez, José Manuel
Journal: Talanta (2020): 120860
Spin-column micro-solid phase extraction of chlorophenols using MFU-4l metal-organic framework.
Authors: Esrafili, Ali and Ghambarian, Mahnaz and Tajik, Mohammad and Baharfar, Mahroo
Journal: Mikrochimica acta (2019): 39
Synthesis and characterization of polyamide-graphene oxide-polypyrrole electrospun nanofibers for spin-column micro solid phase extraction of parabens in milk samples.
Authors: Seidi, Shahram and Sadat Karimi, Elnaz and Rouhollahi, Ahmad and Baharfar, Mahroo and Shanehsaz, Maryam and Tajik, Mohammad
Journal: Journal of chromatography. A (2019): 25-34
Filter paper-based spin column method for cost-efficient DNA or RNA purification.
Authors: Shi, Rui and Lewis, Ramsey S and Panthee, Dilip R
Journal: PloS one (2018): e0203011
A rapid silica spin column-based method of RNA extraction from fruit trees for RT-PCR detection of viruses.
Authors: Yang, Fan and Wang, Guoping and Xu, Wenxing and Hong, Ni
Journal: Journal of virological methods (2017): 61-67
A spin column-free approach to sodium hydroxide-based glycan permethylation.
Authors: Hu, Yueming and Borges, Chad R
Journal: The Analyst (2017): 2748-2759
A Rapid Spin Column-Based Method to Enrich Pathogen Transcripts from Eukaryotic Host Cells Prior to Sequencing.
Authors: Bent, Zachary W and Poorey, Kunal and LaBauve, Annette E and Hamblin, Rachelle and Williams, Kelly P and Meagher, Robert J
Journal: PloS one (2016): e0168788
Characterization of RNA from Exosomes and Other Extracellular Vesicles Isolated by a Novel Spin Column-Based Method.
Authors: Enderle, Daniel and Spiel, Alexandra and Coticchia, Christine M and Berghoff, Emily and Mueller, Romy and Schlumpberger, Martin and Sprenger-Haussels, Markus and Shaffer, Jonathan M and Lader, Eric and Skog, Johan and Noerholm, Mikkel
Journal: PloS one (2015): e0136133
Development of a silica monolith modified with Fe3O4 nano-particles in centrifugal spin column format for the extraction of phosphorylated compounds.
Authors: Alwy, Ali and Clarke, Sarah P and Brougham, Dermot F and Twamley, Brendan and Paull, Brett and White, Blánaid and Connolly, Damian
Journal: Journal of separation science (2015): 283-90
Heterogeneous catalysis for azide-alkyne bioconjugation in solution via spin column: Attachment of dyes and saccharides to peptides and DNA.
Authors: Kallick, Jeremy and Harris, Sarah and Udit, Andrew K and Hill, Michael G
Journal: BioTechniques (2015): 329-30, 332, 334