在过去的一些年，Cy5荧光基因是生命科学研究和诊断领域非常受欢迎的标记试剂。在红色区域有最大的荧光发射，许多CCD检测器有最大灵敏性，并且背景低。染料颜色非常深，因此即使在凝胶上少至1nmol的量用肉眼也可观察到。

Cy5 NHS酯是用来标记多肽，蛋白和寡核苷酸氨基的活性染料。此染料只需少量的共溶剂（比如DMF或DMSO）来标记反应。此试剂用来高效标记可溶性蛋白，各种多肽和寡核苷酸的氨基。对于较复杂的靶标比如易降解的蛋白，不能用DMF或DMSO时，可以考虑用水溶性sulfo-Cyanine 5 NHS 酯不需要共溶剂，并且两者有非常相似的荧光特性。

Cy5荧光基因与各种设备兼容包括许多荧光显微镜，成像仪，扫描设备和荧光成像仪等。

   Cy5 NHS酯可以替代Alexa Fluor 647, and DyLight 649和Cy3.5®活性酯。

外观：深蓝色粉末

分子量：616.19

分子式：C₃₆H₄₂ClN₃O₄

CAS号：1032678-42-4, 350686-88-3

溶解性：易溶于有机溶剂（DMSO,DMF和氯化物），水中的溶解性低。

质控：NMR 1H（95%）和13C，TLC, 功能测试

储存条件：储存：收到货后于-20℃避光保存24个月。运输：室温3周，避免长时间暴露于阳光，干燥。

最大激发波长：646

在最大激发波长下的消光系数：250000

最大发射波长：662

荧光量子产率：0.2

During the last years, Cyanine5 (an analog of Cy5^®) has become an incredibly popular label in life science research and diagnostics. The fluorophore has its emission maximum in the red region, where many CCD detectors exhibit maximum sensitivity, and biological objects show low background. The dye color is very intense, therefore quantities as small as 1 nmol can be detected in gel electrophoresis by naked eye.

This Cyanine5 NHS ester (analog to Cy5^® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires a small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reactions (please see our recommended protocol for more details). This reagent is ideal for very cost-efficient labeling of soluble proteins as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. For more sophisticated targets such as easily degradable proteins, when the use of DMF or DMSO is undesirable, consider using water-soluble sulfo-Cyanine 5 NHS ester which does not require any co-solvent, and features very similar fluorescent properties.