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Portelite 快速荧光法内毒素检测试剂盒
货号 | 60009 | 存储条件 | Multiple |
规格 | 500 Tests | 价格 | 17628 |
Ex (nm) | Em (nm) | ||
分子量 | 溶剂 | ||
产品详细介绍 |
简要概述
产品基本信息
货号:60009
产品名称:Portelite 快速荧光法内毒素检测试剂盒
规格:500 Tests
储存条件:-15℃避光防潮
保质期:24个月
试剂盒组分
组分A:Endotoxin Green(1瓶)
组分B:不含内毒素溶液(1瓶,20ml)
组分C:Li变形细胞(1瓶)
组分D:大肠杆菌内毒素标准品(10 EU)
组分E:DMSO(1瓶,100ul)
适用仪器
CytoCite 荧光计 | |
激发: | 480nm |
发射: | 510-580nm |
器材: | 0.2 mL薄壁PCR管 |
产品介绍
脂多糖(LPS),也称为内毒素,是革兰氏阴性细菌外膜的主要成分。 LPS是脊椎动物先天免疫系统的有效刺激剂,可引起发烧,败血性休克并最终导致死亡。 LPS还被认为是检测细菌病原体入侵的生物标记,在极端情况下还负责炎症反应和内毒素休克的发展。检测生物材料(例如蛋白质,肽或抗体样品)中的LPS是生物制造和加工中的关键任务。 Portelite 快速荧光内毒素检测试剂盒使用Endotoxin Green (一种敏感的荧光底物)。Endotoxin Green可以在存在内毒素和血红细胞裂解液(LAL)(一种来自crab蟹的血细胞的提取物)的情况下进行水解,产生强烈的绿色荧光。内毒素活性与Endotoxin Green 水解产生的荧光强度成正比。该试剂盒针对Cytocite 和Qubit 荧光计进行了优化,可以检测样品中存在的多种内毒素(从1 EU / ml到0.002EU / ml)。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Portelite 快速荧光法内毒素检测试剂盒。
图示
图1. Portelite 快速荧光内毒素检测试剂盒图 |
参考文献
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