Gelite Safe核酸凝胶染料* 10,000X DMSO溶液*

	货号	17707	存储条件	室温储存不要冷冻, 避免光照
	规格	10 ml	价格	11400
	Ex (nm)	513	Em (nm)	552
	分子量	N/A	溶剂	Water
	产品详细介绍

简要概述

Gelite Safe 核酸凝胶染料是一种非常敏感且稳定的荧光染料，可用于电泳凝胶中的DNA检测。 与高毒性的溴化乙锭（EtBr）不同，Gelite Safe经过专门设计，具有较低的危害性，无细胞毒性和诱变性，与EtBr和SYBR®Safe相比，具有更高的灵敏度和更低的背景荧光。 Gelite Safe的独特光谱特性扩展了其仪器兼容性，包括紫外和蓝光透射仪，凝胶记录系统和激光扫描仪。无需繁杂的操作，可在在绿色或红色通道中进行检测。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Gelite Safe核酸凝胶染料* 10,000X DMSO溶液*。 

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产品说明书

样品实验方案

工作溶液配制

Gelite Safe 工作溶液

在7.5-8.5的pH范围内使用（例如，TAE、TBE或TE pH 8.2）缓冲液稀释10,000X储备试剂来制备1X Gelite Safe工作溶液。
注意：在水中配制的染色溶液比在缓冲液中配制的溶液不稳定，必须在24小时内使用。

后染色方案
1.根据您的标准方案运行凝胶。
2.将凝胶放在合适的聚丙烯容器中。 轻轻加入足量的1X染色溶液以浸没凝胶。
注意：请勿使用玻璃容器，因为它会吸收染色溶液中的许多染料。
3.在室温下避光轻轻搅拌凝胶约30至60分钟。
4.使用300 nm / 254 nm紫外透射仪或使用长距离绿色滤光片（例如SYBR®滤光片，GelStar®滤光片，GelGreen®滤光片或GelRed®滤光片）的凝胶扫描仪对凝胶成像。

预染色方案
1.使用标准方案准备琼脂糖凝胶溶液。
2.在倒入凝胶之前，将10,000X Gelite Safe储备试剂以1：10,000的比例稀释到凝胶溶液中并彻底混合。
3.根据您的标准方案运行凝胶。
4.使用300 nm / 254 nm紫外透射仪或使用长距离绿色滤光片（例如SYBR®滤光片，GelStar®滤光片，GelGreen®滤光片或GelRed®滤光片）的凝胶扫描仪对凝胶成像。

图示

图1.使用Gelite Safe，EtBr和SYBR®Safe在TBE缓冲液中的1％琼脂糖凝胶中检测DNA的比较。 从左到右分别以100 ng，50 ng和25 ng的量加载1 kb DNA的两倍连续稀释液。 根据制造商推荐的浓度，将凝胶用Gelite Safe，EtBr和SYBR®Safe染色60分钟，并使用ChemiDoc 成像系统（Bio-Rad®）进行成像。 使用装有GelGreen和GelRed滤光片的300 nm荧光透射仪对凝胶进行照明。

参考文献

Limited Proteolysis and Gel Electrophoresis in the Presence of Metal Cations: Au(III)-binding Luminescent Domain in Serum Albumins.
Authors: Dixon, Jacob M and Egusa, Shunji
Journal: Journal of visualized experiments : JoVE (2021)

High affinity of AS1411 toward copper; its application in a sensitive aptasensor for copper detection.
Authors: Bahreyni, Amirhossein and Ramezani, Mohammad and Alibolandi, Mona and Hassanzadeh, Pirooz and Abnous, Khalil and Taghdisi, Seyed Mohammad
Journal: Analytical biochemistry (2019): 1-9

The efficacy and tolerability of 5-aminolevulinic acid 5% thermosetting gel photodynamic therapy (PDT) in the treatment of mild-to-moderate acne vulgaris. A two-center, prospective assessor-blinded, proof-of-concept study.
Authors: Serini, Stefano Maria and Cannizzaro, Maria Vittoria and Dattola, Annunziata and Garofalo, Virginia and Del Duca, Esther and Ventura, Alessandra and Milani, Massimo and Campione, Elena and Bianchi, Luca
Journal: Journal of cosmetic dermatology (2019): 156-162

Transfection Studies with Colloidal Systems Containing Highly Purified Bipolar Tetraether Lipids from Sulfolobus acidocaldarius.
Authors: Engelhardt, Konrad H and Pinnapireddy, Shashank Reddy and Baghdan, Elias and Jedelská, Jarmila and Bakowsky, Udo
Journal: Archaea (Vancouver, B.C.) (2017): 8047149

Metallo-supramolecular gels based on a multitopic cyclam bis-terpyridine platform.
Authors: Gasnier, Aurélien and Royal, Guy and Terech, Pierre
Journal: Langmuir : the ACS journal of surfaces and colloids (2009): 8751-62

Characterization of hepatic L-threonine dehydrogenase of chicken.
Authors: Yuan, J H and Austic, R E
Journal: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology (2001): 65-73

[Cloning of group A streptococcal pyrogenic exotoxin-B gene and its recombinant protein expression in culture supernatant].
Authors: Watanabe, Y
Journal: Journal of Nippon Medical School = Nippon Ika Daigaku zasshi (2001): 222-32

Purification and characterization of chondroitin 4-sulfotransferase from the culture medium of a rat chondrosarcoma cell line.
Authors: Yamauchi, S and Hirahara, Y and Usui, H and Takeda, Y and Hoshino, M and Fukuta, M and Kimura, J H and Habuchi, O
Journal: The Journal of biological chemistry (1999): 2456-63

Comparison of tube and gel red blood cell agglutination techniques in detecting chimeras after major ABO-mismatched allogeneic hematopoietic stem cell transplantation.
Authors: Kupferman, M J and Cipolone, K M and Procter, J L and Stroncek, D F
Journal: Immunohematology (1998): 63-7

Expression of pig heart mitochondrial NADP-dependent isocitrate dehydrogenase in Escherichia coli.
Authors: Soundar, S and Jennings, G T and McAlister-Henn, L and Colman, R F
Journal: Protein expression and purification (1996): 305-12