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Xylanase Assay Kit (XylX6 Method) 
Product code: K-XylX6-2V
€312.00
200 assays (manual) / 400 assays (auto-analyser)
Prices exclude VAT
| Content: | (K-XylX6-1V) 100 assays (manual) / 200 assays (auto-analyser) (K-XylX6-2V) 200 assays (manual) / 400 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | endo-1,4-β-Xylanase |
| Assay Format: | Spectrophotometer, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400, 405 |
| Signal Response: | Increase |
| Limit of Detection: | 5.3 x 10-4 U/mL |
| Reproducibility (%): | ~ 3% |
| Total Assay Time: | 10 min |
| Application examples: | Fermentation broths, industrial enzyme preparations, animal feed, biofuels research, barley malt analysis. |
| Method recognition: | Novel method |
The XylX6 assay reagent for the measurement of endo-xylanase (endo-1,4-β-xylanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-45-glucosyl-xylopentaoside and 2) β-xylosidase. The ketone blocking group prevents any hydrolytic action by the β-xylosidase or other exo-acting glycosidases on the XylX6 substrate. Incubation with an endo-xylanase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-xylosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of XylX6 by the endo-xylanase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).
Need to measure other enzyme activities? View our complete list of assay kits for measurement of enzyme activity.
Note that standard curves relating the absorbance obtained using the XylX6 assay to endo-xylanase activity on the native substrates, wheat arabinoxylan and beechwood xylan, are provided in the Supporting Information file under the Documents tab.
Advantages
- Very cost effective
- All reagents stable for > 4 years
- Completely specific for endo-1,4-β-xylanase
- Generally applicable and highly sensitive
- Simple format. Well suited to automation
- Excellent reproducibility
- Standard included
Related products
Similar Products
K-AZOWAX – Xylanase Assay Kit (Azo-Wax) K-XYLS – Xylanase Assay Kit (Xylazyme AX)
Related Carbohydrates
P-XYLNBE-10G – Xylan (Beechwood) P-WAXYL – Arabinoxylan (Wheat Flour; Low Viscosity) P-WAXYM – Arabinoxylan (Wheat Flour; Medium Viscosity) P-WAXYH – Arabinoxylan (Wheat Flour; High Viscosity) P-WAXYRS – Arabinoxylan (Wheat Flour; for Reducing Sugar Assays) P-WAXYI – Arabinoxylan (Wheat Flour; Insoluble) P-ADWAX22 – Wheat Arabinoxylan (acid debranched, 22% Ara) P-EDWAX30 – Wheat Arabinoxylan (enzyme debranched, 30% Ara) P-RAXY – Arabinoxylan (Rye Flour) O-XBI – Xylobiose O-XTR – Xylotriose O-XTE – Xylotetraose O-XPE – Xylopentaose O-XHE – Xylohexaose O-A3X – 32-α-L-Arabinofuranosyl-xylobiose (A3X) O-A2XX – 23-α-L-Arabinofuranosyl-xylotriose (A2XX) O-A23XX-30MG – 23,33-di-α-L-Arabinofuranosyl-xylotriose (A2,3XX) O-XA3XX – 33-α-L-Arabinofuranosyl-xylotetraose (XA3XX) O-XAXXMIX-10MG – 33-α-L- plus 23-α-L-Arabinofuranosyl-xylotetraose (XA3XX/XA2XX) mixture
Colourimetric Substrates
T-XYZ-200T – Xylazyme Tablets T-XAX-200T – Xylazyme AX Tablets S-AWAXL – Azo-Wheat Arabinoxylan (Liquid) S-AWAXP – Azo-Wheat Arabinoxylan (Powder) S-AXBL – Azo-Xylan (Birchwood) (Liquid) S-AXBP – Azo-Xylan (Birchwood) (Powder) I-AZXBE – AZCL-Xylan (Beechwood) I-AZWAX – AZCL-Arabinoxylan (Wheat) I-AZWAXF – AZCL-Arabinoxylan (Wheat) (fine) O-PNPX2-10MG – 4-Nitrophenyl-β-xylobioside O-PNPX3 – 4-Nitrophenyl-β-xylotrioside O-4MUX2-5MG – 4-Methylumbelliferyl-β-xylobioside
Associated Enzymes
E-XYTR1 – endo-1,4-β-Xylanase M1 (Trichoderma viride) E-XYTR3 – endo-1,4-β-Xylanase M3
(Trichoderma longibrachiatum) E-XYAN4 – endo-1,4-β-Xylanase M4 (Aspergillus niger) E-XYRU6 – endo-1,4-β-Xylanase (rumen microorganism) E-XYNBS – endo-1,4-β-Xylanase
(Bacillus stearothermophilus T6) E-XYNACJ – endo-1,4-β-Xylanase (Cellvibrio japonicus) E-XYLATM – endo-1,4-β-Xylanase (Thermotoga maritima) E-BXSR-1KU – β-D-Xylosidase (Selenomonas ruminantium) E-BXSEBP – β-Xylosidase (Bacillus pumilus)
(Trichoderma longibrachiatum) E-XYAN4 – endo-1,4-β-Xylanase M4 (Aspergillus niger) E-XYRU6 – endo-1,4-β-Xylanase (rumen microorganism) E-XYNBS – endo-1,4-β-Xylanase
(Bacillus stearothermophilus T6) E-XYNACJ – endo-1,4-β-Xylanase (Cellvibrio japonicus) E-XYLATM – endo-1,4-β-Xylanase (Thermotoga maritima) E-BXSR-1KU – β-D-Xylosidase (Selenomonas ruminantium) E-BXSEBP – β-Xylosidase (Bacillus pumilus)
Similar Products
K-AZOWAX – Xylanase Assay Kit (Azo-Wax) K-XYLS – Xylanase Assay Kit (Xylazyme AX)
Related Carbohydrates
P-XYLNBE-10G – Xylan (Beechwood) P-WAXYL – Arabinoxylan (Wheat Flour; Low Viscosity) P-WAXYM – Arabinoxylan (Wheat Flour; Medium Viscosity) P-WAXYH – Arabinoxylan (Wheat Flour; High Viscosity) P-WAXYRS – Arabinoxylan (Wheat Flour; for Reducing Sugar Assays) P-WAXYI – Arabinoxylan (Wheat Flour; Insoluble) P-ADWAX22 – Wheat Arabinoxylan (acid debranched, 22% Ara) P-EDWAX30 – Wheat Arabinoxylan (enzyme debranched, 30% Ara) P-RAXY – Arabinoxylan (Rye Flour) O-XBI – Xylobiose O-XTR – Xylotriose O-XTE – Xylotetraose O-XPE – Xylopentaose O-XHE – Xylohexaose O-A3X – 32-α-L-Arabinofuranosyl-xylobiose (A3X) O-A2XX – 23-α-L-Arabinofuranosyl-xylotriose (A2XX) O-A23XX-30MG – 23,33-di-α-L-Arabinofuranosyl-xylotriose (A2,3XX) O-XA3XX – 33-α-L-Arabinofuranosyl-xylotetraose (XA3XX) O-XAXXMIX-10MG – 33-α-L- plus 23-α-L-Arabinofuranosyl-xylotetraose (XA3XX/XA2XX) mixture
Colourimetric Substrates
T-XYZ-200T – Xylazyme Tablets T-XAX-200T – Xylazyme AX Tablets S-AWAXL – Azo-Wheat Arabinoxylan (Liquid) S-AWAXP – Azo-Wheat Arabinoxylan (Powder) S-AXBL – Azo-Xylan (Birchwood) (Liquid) S-AXBP – Azo-Xylan (Birchwood) (Powder) I-AZXBE – AZCL-Xylan (Beechwood) I-AZWAX – AZCL-Arabinoxylan (Wheat) I-AZWAXF – AZCL-Arabinoxylan (Wheat) (fine) O-PNPX2-10MG – 4-Nitrophenyl-β-xylobioside O-PNPX3 – 4-Nitrophenyl-β-xylotrioside O-4MUX2-5MG – 4-Methylumbelliferyl-β-xylobioside
Associated Enzymes
E-XYTR1 – endo-1,4-β-Xylanase M1 (Trichoderma viride) E-XYTR3 – endo-1,4-β-Xylanase M3
(Trichoderma longibrachiatum) E-XYAN4 – endo-1,4-β-Xylanase M4 (Aspergillus niger) E-XYRU6 – endo-1,4-β-Xylanase (rumen microorganism) E-XYNBS – endo-1,4-β-Xylanase
(Bacillus stearothermophilus T6) E-XYNACJ – endo-1,4-β-Xylanase (Cellvibrio japonicus) E-XYLATM – endo-1,4-β-Xylanase (Thermotoga maritima) E-BXSR-1KU – β-D-Xylosidase (Selenomonas ruminantium) E-BXSEBP – β-Xylosidase (Bacillus pumilus)
(Trichoderma longibrachiatum) E-XYAN4 – endo-1,4-β-Xylanase M4 (Aspergillus niger) E-XYRU6 – endo-1,4-β-Xylanase (rumen microorganism) E-XYNBS – endo-1,4-β-Xylanase
(Bacillus stearothermophilus T6) E-XYNACJ – endo-1,4-β-Xylanase (Cellvibrio japonicus) E-XYLATM – endo-1,4-β-Xylanase (Thermotoga maritima) E-BXSR-1KU – β-D-Xylosidase (Selenomonas ruminantium) E-BXSEBP – β-Xylosidase (Bacillus pumilus)
