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Amyloglucosidase (Aspergillus niger) Powder
4 g – 36,000 Units/g
Prices exclude VAT
Content: | 4 g – 36,000 Units/g |
Shipping Temperature: | Ambient |
Storage Temperature: | Below -10oC |
Formulation: | Supplied as a lyophilised powder |
Physical Form: | Powder |
Stability: | Minimum 1 year at < -10oC. Check vial for details. |
Enzyme Activity: | Amyloglucosidase |
EC Number: | 3.2.1.3 |
CAZy Family: | GH15 |
Synonyms: | 4-alpha-D-glucan glucohydrolase; glucoamylase |
Source: | Aspergillus niger |
Molecular Weight: | 143,500 |
Expression: | From Aspergillus niger |
Specificity: | Hydrolysis of terminal α-1,4 and α-1,6 D-glucose residues successively from non-reducing ends of maltodextrins. |
Specific Activity: | ~ 36,000 U/g (40oC, pH 4.5, soluble starch as substrate). ~ 3,100 U/g on p-nitrophenyl β-maltoside |
Unit Definition: | One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one µmole of D-glucose reducing-sugar equivalents per minute from soluble starch at pH 4.5 and 40oC. |
Temperature Optima: | 70oC |
pH Optima: | 4 |
Method recognition: | AOAC Method 2001.03, AOAC Method 2000.11, AOAC Method 2016.06, GB Standards 5009.255-2016 and ISO Standard 15914:2004 |
High purity Amyloglucosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Amyloglucosidase. From A. niger. High purity. For use in Megazyme Total Starch and Dietary Fiber methods. Specifically prepared for use in AOAC Method 2001.03 which requires enzymes free of glycerol. This AMG is used in combination with thermostable α-amylase (Cat. No. E-BLAAM) and protease powder (Cat. No. E-BSPRPD). For use, the AMG is dissolved in water at a rate of 1 g/10 mL and 0.2 mL is used per assay. Electrophoretically homogeneous. Lyophilised powder.
Also suitable for use in AOAC 2001.03, AOAC Method 2000.11, AOAC Method 2016.06, GB Standards 5009.255-2016 and ISO Standard 15914:2004.
We have extensive choices for different Carbohydrate Active enZYmes.
Validation of Methods
Glycerol Free E-AMGFR-100MG – Amyloglucosidase (Aspergillus niger) E-AMGPU – Amyloglucosidase (Rhizopus sp.) E-GAMP – Glucoamylase P (H. resinae)
Glycerol Free E-AMGFR-100MG – Amyloglucosidase (Aspergillus niger) E-AMGPU – Amyloglucosidase (Rhizopus sp.) E-GAMP – Glucoamylase P (H. resinae)