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Non-specific endo-1,3(4)-β-Glucanase
(Clostridium thermocellum)
Product code: E-LICACT
€228.00
5,000 Units
Prices exclude VAT
Content: | 5,000 Units |
Shipping Temperature: | Ambient |
Storage Temperature: | Below -10oC |
Formulation: | In 50% (v/v) glycerol |
Physical Form: | Solution |
Stability: | Minimum 1 year at 4oC. Check vial for details. |
Enzyme Activity: | β-Glucanase/Lichenase |
EC Number: | 3.2.1.6 |
CAZy Family: | GH16 |
CAS Number: | 62213-14-3 |
Synonyms: | endo-1,3(4)-beta-glucanase; 3(or 4)-beta-D-glucan 3(4)-glucanohydrolase |
Source: | Clostridium thermocellum |
Molecular Weight: | 29,300 |
Concentration: | Supplied at ~ 1,250 U/mL |
Expression: | Recombinant from Clostridium thermocellum |
Specificity: | endo-hydrolysis of (1,3)- or (1,4)-linkages in β-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolysed is itself substituted at C-3. Substrates include laminarin, lichenin and cereal D-glucans. |
Specific Activity: | ~ 186 U/mg (40oC, pH 6.5 on barley β-glucan); ~ 395 U/mg (60oC, pH 6.5 on barley β-glucan); ~ 7.2 U/mg (40oC, pH 6.5 on CM-curdlan) |
Unit Definition: | One Unit of glucanase activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar equivalents per minute from barley β-glucan (5 mg/mL) in sodium phosphate buffer (100 mM), pH 6.5 at 40oC. |
Temperature Optima: | 60oC |
pH Optima: | 6.5 |
Application examples: | Applications in carbohydrate and biofuels research and in the food and feeds industries. |
High purity recombinant Non-specific endo-1,3(4)-β-Glucanase (Clostridium thermocellum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
See our complete CAZy enzyme products list.
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E-LICHN – Lichenase (endo-1,3:1,4-β-D-Glucanase)
(Bacillus subtilis)
(Bacillus subtilis)
Related Carbohydrates
P-BGBL – β-Glucan (Barley; Low Viscosity) P-BGBM – β-Glucan (Barley; Medium Viscosity) P-BGBH – β-Glucan (Barley; High Viscosity) P-BGOM – β-Glucan (Oat; Medium Viscosity) P-BGOH – β-Glucan (Oat; High Viscosity) P-BGCFA – β-Glucan CFA Standard P-MWBGS – β-Glucan MW Standards P-LICHN – Lichenan (Icelandic Moss) O-BGTRIA – 32-β-D-Glucosyl-cellobiose O-BGTRIB – 31-β-D-Cellobiosyl-glucose O-BGTETB – 31-β-D-Cellotriosyl-glucose O-BGTETC – 32-β-D-Cellobiosyl-cellobiose +
33-β-D-Glucosyl-cellotriose
33-β-D-Glucosyl-cellotriose
Colourimetric Substrates
K-MBG4 – Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) T-BGZ-200T – β-Glucazyme Tablets S-ABG100 – Azo-Barley Glucan I-AZBGL – AZCL-Barley β-Glucan
Associated Enzymes
E-EXBGTV – exo-1,3-β-D-Glucanase (Trichoderma virens) E-EXG5AO – exo-1,3-β-D-Glucanase (Aspergillus oryzae) E-BGLUC – β-Glucosidase (Aspergillus niger) E-BGOSTM – β-Glucosidase (Thermotoga maritima) E-BGOSPC – β-Glucosidase (Phanerochaete chrysosporium) E-BGOSAG – β-Glucosidase (Agrobacterium sp.)
Similar Products
E-LICHN – Lichenase (endo-1,3:1,4-β-D-Glucanase)
(Bacillus subtilis)
(Bacillus subtilis)
Related Carbohydrates
P-BGBL – β-Glucan (Barley; Low Viscosity) P-BGBM – β-Glucan (Barley; Medium Viscosity) P-BGBH – β-Glucan (Barley; High Viscosity) P-BGOM – β-Glucan (Oat; Medium Viscosity) P-BGOH – β-Glucan (Oat; High Viscosity) P-BGCFA – β-Glucan CFA Standard P-MWBGS – β-Glucan MW Standards P-LICHN – Lichenan (Icelandic Moss) O-BGTRIA – 32-β-D-Glucosyl-cellobiose O-BGTRIB – 31-β-D-Cellobiosyl-glucose O-BGTETB – 31-β-D-Cellotriosyl-glucose O-BGTETC – 32-β-D-Cellobiosyl-cellobiose +
33-β-D-Glucosyl-cellotriose
33-β-D-Glucosyl-cellotriose
Colourimetric Substrates
K-MBG4 – Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) T-BGZ-200T – β-Glucazyme Tablets S-ABG100 – Azo-Barley Glucan I-AZBGL – AZCL-Barley β-Glucan
Associated Enzymes
E-EXBGTV – exo-1,3-β-D-Glucanase (Trichoderma virens) E-EXG5AO – exo-1,3-β-D-Glucanase (Aspergillus oryzae) E-BGLUC – β-Glucosidase (Aspergillus niger) E-BGOSTM – β-Glucosidase (Thermotoga maritima) E-BGOSPC – β-Glucosidase (Phanerochaete chrysosporium) E-BGOSAG – β-Glucosidase (Agrobacterium sp.)