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exo-α-Sialidase (Clostridium perfringens) 
Product code: E-SIALCP
€197.00
50 Units
Prices exclude VAT
| Content: | 50 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 20 mM tris buffer containing 50 mM NaCl2 and 5 mM EDTA |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. Check vial for details. |
| Enzyme Activity: | α-Sialidase |
| EC Number: | 3.2.1.18 |
| CAZy Family: | GH33 |
| CAS Number: | 9001-67-6 |
| Synonyms: | exo-alpha-sialidase; acetylneuraminyl hydrolase |
| Source: | Clostridium perfringens |
| Molecular Weight: | 43,600 |
| Concentration: | Supplied at ~ 500 U/mL |
| Expression: | Recombinant from Clostridium perfringens |
| Specificity: | Hydrolysis of unbranched, non-reducing terminal α-2,3-linked, α-2,6-linked >> α-2,8-linked N-acetylneuraminic acid (NANA; Neu5Ac) residues from glycoproteins and oligosaccharides of glycoconjugates. |
| Specific Activity: | ~ 300 U/mg (37oC, pH 7.0 on pNP-α-D-N-acetylneuraminic acid) |
| Unit Definition: | One Unit of sialidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from pNP-α-D-N-acetylneuraminic acid (1 mM) in sodium phosphate buffer (100 mM), pH 7.0 at 37oC. |
| Temperature Optima: | 37oC |
| pH Optima: | 7 |
| Application examples: | Applications in clinical chemistry, glycobiology and carbohydrate research. |
High purity recombinant exo-α-sialidase (Clostridium perfringens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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