Magazyme试剂盒Malt beta-Glucanase-Lichenase Assay Kit MBG4 Method K-MBG4

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Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) Malt beta-Glucanase-Lichenase Assay Kit MBG4 Method K-MBG4 Malt beta-Glucanase-Lichenase Assay Kit MBG4 Method K-MBG4 Malt beta-Glucanase-Lichenase Assay Kit MBG4 Method K-MBG4

Product code: K-MBG4

€242.00

100 assays (manual) / 400 assays (auto-analyser)

Prices exclude VAT

  • Assay Protocol
  • SDS
  • Mega-Calc
  • COAs
  • FAQs
    • Content: (Malt β-glucanase) 100 assays (manual) / 400 (auto-analyser) Or
    (Lichenase) 100 / 200 assays (manual) / 330 (auto-analyser)
    Shipping Temperature: Ambient
    Storage Temperature: Short term stability: 2-8oC,
    Long term stability: See individual component labels
    Stability: > 2 years under recommended storage conditions
    Analyte: β-Glucanase/Lichenase
    Assay Format: Spectrophotometer, Auto-analyser
    Detection Method: Absorbance
    Wavelength (nm): 400
    Signal Response: Increase
    Limit of Detection: (Malt β-glucanase) 4.3 x 10-4 U/mL Or
    (Lichenase) 9.1 x 10-5 U/mL
    Reproducibility (%): ~ 3%
    Total Assay Time: (Malt β-glucanase) ~ 20 min Or
    (Lichenase) ~ 10 min 
    Application examples: Crude malt extracts, industrial enzyme preparations.
    Method recognition: Novel method

    The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.

    Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).

    Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.

    Discover more assay kits for enzyme activity measurement.

    Data calculators are located in the Documents tab.

    Malt beta-Glucanase-Lichenase Assay Kit MBG4 Method K-MBG4

    Advantages
    • Very cost effective 
    • All reagents stable for > 2 years 
    • Specific for endo-1,3:1,4-β-glucanase/lichenase 
    • Simple, convenient, rapid assay 
    • Well suited to automation 
    • Malt flour standard and lichenase standard included

    Related products
    Similar Products
    K-MBGL – β-Glucanase Assay Kit (Malt and Microbial)

    Related Carbohydrates
    P-BGBL – β-Glucan (Barley; Low Viscosity) P-BGBM – β-Glucan (Barley; Medium Viscosity) P-BGBH – β-Glucan (Barley; High Viscosity) P-BGOM – β-Glucan (Oat; Medium Viscosity) P-BGOH – β-Glucan (Oat; High Viscosity) P-BGCFA – β-Glucan CFA Standard P-MWBGS – β-Glucan MW Standards P-LICHN – Lichenan (Icelandic Moss) O-BGTRIA – 32-β-D-Glucosyl-cellobiose O-BGTRIB – 31-β-D-Cellobiosyl-glucose O-BGTETB – 31-β-D-Cellotriosyl-glucose O-BGTETC – 32-β-D-Cellobiosyl-cellobiose +
    33-β-D-Glucosyl-cellotriose

    Colourimetric Substrates
    T-BGZ-200T – β-Glucazyme Tablets S-ABG100 – Azo-Barley Glucan I-AZBGL – AZCL-Barley β-Glucan

    Associated Enzymes
    E-LICACT – Non-specific endo-1,3(4)-β-Glucanase
    (Clostridium thermocellum) E-LICHN – Lichenase (endo-1,3:1,4-β-D-Glucanase)
    (Bacillus subtilis) E-CELTR – Cellulase (endo-1,4-β-D-glucanase)
    (Trichoderma longibrachiatum)