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Products > Crystallization Screens > PCT Pre-Crystallization Test > PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
Applications
- Determine the appropriate sample concentration for crystallization screening
Features
- Conserve sample
- Optimize sample concentration prior to initial screens
- Provide insight to sample homogeneity
Description
The PCT Pre-Crystallization Test is used to determine the appropriate protein concentration for crystallization screening. Sample concentration is a significant crystallization variable. Samples too concentrated can result in amorphous precipitate, while samples too dilute can result in clear drops. Precipitate and clear drops are typical crystallization screen results for reagent conditions which do not promote crystallization and are part of every crystallization screen. However, by optimizing protein concentration for the screen, the number of clear and precipitate results can often be reduced, which in turn results in more efficient sample utilization while at the same time enhancing the chances for crystallization. PCT can minimize or prevent situations where a screen results in an over abundance of precipitate or clear drops.
The PCT kit contains 4 unique, preformulated, sterile filtered reagents used to evaluate protein concentration for crystallization screening. Initially, the sample protein is mixed with two of the reagents to determine if the protein concentration is appropriate for crystallization screening. If the protein is very sensitive to salt and polymer concentration, based on initial PCT results, the protein may be evaluated using a second set of PCT reagents. PCT results will then provide insight to either the appropriate sample concentration or indicate that other diagnostic testing such as native gel electrophoresis or dynamic light scattering should be performed to demonstrate sample homogeneity appropriate for crystallization.
PCT Kit comparison table.
| Catalog Number |
4 Unique Reagents |
Glass Siliconized Cover Slides |
24 Well Plate with sealant |
| HR2-140 | ✓ (50 mL each) | ✓ (22 mm Square) | |
| HR2-141 | ✓ (10 mL each) | ||
| HR2-142 | ✓ (30 mL each) | ✓ (22 mm Square) | ✓ (5x VDX) |
| HR2-143 | ✓ (10 mL each) | ✓ (18 mm Circle) | ✓ (4x VDXm) |
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CAT NO
HR2-140
NAME
DESCRIPTION
50 ml bottles (4 ea), cover slides (1 pk)
PRICE
$100.00
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CAT NO
HR2-141
NAME
DESCRIPTION
10 ml tubes (4 ea)
PRICE
$77.00
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CAT NO
HR2-142
NAME
DESCRIPTION
30 ml bottles (4 ea), cover slides (1 pk), VDX Plates with sealant (5 ea)
PRICE
$121.00
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CAT NO
HR2-143
NAME
DESCRIPTION
10 ml tubes (4 ea), cover slides (1 pk), VDXm Plates with sealant (4 ea)
PRICE
$99.00
cart quote
Support Material(s)
HR2-140 & HR2-141 & HR2-142 & HR2-143 PCT Kit Documents
HR2-140 PCT Kit (with slides) SDS
HR2-141 PCT Kit (reagents only) SDS
HR2-142 PCT Kit (with plates and slides) SDS
HR2-143 PCT Kit (with plates and slides) SDS Related Item(S)
- Individual PCT Reagents
References
1. Crystallization and x-ray diffraction analysis of human CLEC-2. Aleksandra A. Watson and Christopher A. O’Callaghan. Acta Cryst. (2005). F61, 1094-1096.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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