Hampton Research蛋白结晶试剂盒
Products > Optimization Screens > StockOptions Buffer Kits > StockOptions Tris Buffer Kit
StockOptions Tris Buffer Kit
Applications
- Focused, titrated buffer stocks for crystal screening, optimization, & production
Features
- pH 7.0 – 9.0 (21 reagents)
- 0.1 pH increments
- 1.0 M concentrated stocks
- Tris pKa = 8.1
Description
StockOptions Tris buffer kit is a preformulated, sterile filtered set of titrated buffer stocks. The StockOptions Tris buffer stock reagents are supplied as 1.0 M stock solutions in 10 ml volumes. Each StockOptions Tris buffer reagent is carefully titrated using hydrochloric acid. StockOptions Tris is comprised of 21 unique reagents covering the pH range of 7.0 to 9.0 in 0.1 pH unit increments.
Tris
Synonym: Trizma base or 2-Amino-2-(hydroxymethyl)-1,3-propanediol or THAM or Tris base or Tris(hydroxymethyl)aminomethane or Trometamol
C4H11NO3 NH2C(CH2OH)3
Mr 121.14
CAS Number [77-86-1]
EC Number 201-064-4
Beilstein Registry Number 741883
Merck 14,9772
RTECS TY2900000
MDL Number MFCD00004679
PubChem Substance ID 24889854
Purity =99.8%
pKa = 8.1 at 25°C
Useful pH range of Tris = 7.0 – 9.0
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CAT NO
HR2-100
NAME
DESCRIPTION
pH 7.0 to 9.0 in 0.1 pH unit increments
PRICE
$243.00
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Support Material(s)
HR2-100 StockOptions Tris Documents
HR2-100 StockOptions Tris SDS Related Item(S)
- Tris Buffer
- Individual StockOptions Tris Reagents
References
1. Microscale Fluorescent Thermal Stability Assay for Membrane Proteins Structure, Volume 16, Issue 3, 11 March 2008, Pages 351-359 Alexander I. Alexandrov, Mauro Mileni, Ellen Y.T. Chien, Michael A. Hanson, Raymond C. Stevens
2. Crystallization and Preliminary X-ray Diffraction Analysis of the ArsC Protein from the Escherichia coli Arsenical Resistance Plasmid, R773. deMel, VSJ; Doyle, MA; Gladysheva, TB; Oden, KL; Martin, PD; Rosen, BP; Edwards, BFP. J Mol Biol 242, 701- 702, 1994.
3. Increasing the size of microcrystals by fine sampling of pH limits. A. McPherson. J. Appl. Cryst. (1995). 28, 362-365.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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