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Products > Custom Shop Crystallization Reagents > Proti-Ace & Proti-Ace 2 > Individual Proti-Ace & Proti-Ace 2 Reagents
Individual Proti-Ace & Proti-Ace 2 Reagents
Applications
- In situ proteolysis and proteolytic screening of protein samples for crystallization and structure determination
- Individual reagents from Proti-Ace & Proti-Ace 2 kits
Features
- Lyophilized enzymes for enhanced stability
Description
Individual reagents from the Proti-Ace and Proti-Ace 2 kits. Each protease is supplied in a stable, lyophilized format in an optimized digest buffer. Simply add water when ready to use.
The unique freeze dried formulation of the Proti-Ace kit offers a much improved protease stability compared to liquid protease formulations. Each tube contains 0.1 mg of lyophilized enzyme and digest buffer as shown below. Add 100 microliters of deionized water (Type 1+) to each tube of lyophilized content to create a 1 mg/ml protease solution in digest buffer. Proti-Ace Dilution buffer is supplied as a ready to use solution.
Proti-Ace Reagent 1* (Qty 3):
• 1 mg/ml a-Chymotrypsin, 1 mM Hydrochloric acid, 2 mM Calcium chloride dihydrate
Proti-Ace Reagent 2* (Qty 3):
• 1 mg/ml Trypsin, 1 mM Hydrochloric acid, 2 mM Calcium chloride dihydrate
Proti-Ace Reagent 3* (Qty 3):
• 1 mg/ml Elastase, 200 mM Tris pH 8.0
Proti-Ace Reagent 4* (Qty 3):
• 1 mg/ml Papain, 2 mM EDTA, 2 mM TCEP hydrochloride, 2 mM L-Cysteine
Proti-Ace Reagent 5* (Qty 3):
• 1 mg/ml Subtilisin, 10 mM Sodium acetate trihydrate pH 7.5, 5 mM Calcium acetate hydrate
Proti-Ace Reagent 6* (Qty 3):
• 1 mg/ml Endoproteinase Glu-C (in deionized water)
* When 100 µl of deionized water is added to each Proti-Ace freeze dried reagent
Proti-Ace Dilution Buffer (Qty 3):
• 1.6 ml of 10 mM HEPES pH 7.5, 500 mM Sodium chloride
Proti-Ace 2 Reagent 1* (Qty 3):
• 1 mg/ml Proteinase K, 10 mM TRIS hydrochloride pH 7.5, 1 mM Calcium acetate hydrate
Proti-Ace 2 Reagent 2* (Qty 3):
• 1 mg/ml Clostripain (Endoproteinase-Arg-C), 10 mM HEPES pH 7.5, 2 mM TCEP hydrochloride, 2 mM L-Cysteine, 1 mM Calcium chloride dihydrate
Proti-Ace 2 Reagent 3* (Qty 3):
• 1 mg/ml Pepsin, 1 mM Hydrochloric acid
Proti-Ace 2 Reagent 4* (Qty 3):
• 1 mg/ml Thermolysin, 10 mM Calcium acetate hydrate pH 8.0, 50 mM Sodium chloride
Proti-Ace 2 Reagent 5* (Qty 3):
• 1 mg/ml Bromelain, 5 mM HEPES pH 7.5, 2 mM L-Cysteine, 1 mM Ethylenediaminetetraacetic acid disodium salt dihydrate
Proti-Ace 2 Reagent 6* (Qty 3):
• 1 mg/ml Actinase, 5 mM HEPES pH 7.5, 10 mM Sodium chloride
* When 100 µl of deionized water is added to each Proti-Ace 2 freeze dried reagent
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CAT NO
HR2-429-01
NAME
DESCRIPTION
a-Chymotrypsin – pack of 3 tubes
PRICE
$65.00
cart quote
CAT NO
HR2-429-02
NAME
DESCRIPTION
Trypsin – pack of 3 tubes
PRICE
$65.00
cart quote
CAT NO
HR2-429-03
NAME
DESCRIPTION
Elastase – pack of 3 tubes
PRICE
$65.00
cart quote
CAT NO
HR2-429-04
NAME
DESCRIPTION
Papain – pack of 3 tubes
PRICE
$65.00
cart quote
CAT NO
HR2-429-05
NAME
DESCRIPTION
Subtilisin – pack of 3 tubes
PRICE
$65.00
cart quote
CAT NO
HR2-429-06
NAME
DESCRIPTION
Endoproteinase Glu-C – pack of 3 tubes
PRICE
$65.00
cart quote
CAT NO
HR2-429-07
NAME
DESCRIPTION
pack of 3 tubes
PRICE
$65.00
cart quote
CAT NO
HR2-432-01
NAME
DESCRIPTION
Proteinase K – pack of 3 tubes
PRICE
$65.00
cart quote
CAT NO
HR2-432-02
NAME
DESCRIPTION
Clostripain (Endoproteinase-Arg-C) – pack of 3 tubes
PRICE
$65.00
cart quote
CAT NO
HR2-432-03
NAME
DESCRIPTION
Pepsin – pack of 3 tubes
PRICE
$65.00
cart quote
CAT NO
HR2-432-04
NAME
DESCRIPTION
Thermolysin – pack of 3 tubes
PRICE
$65.00
cart quote
CAT NO
HR2-432-05
NAME
DESCRIPTION
Bromelain – pack of 3 tubes
PRICE
$65.00
cart quote
CAT NO
HR2-432-06
NAME
DESCRIPTION
Actinase E – pack of 3 tubes
PRICE
$65.00
cart quote
Support Material(s)
HR2-429-** Individual Proti-Ace Reagents SDS
HR2-432-** Individual Proti-Ace 2 Reagents SDS Related Item(S)
- Proti-Ace • Proti-Ace 2
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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