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AF488 is a bright and photostable dye. Due to its high hydrophilicity, this is a dye of choice for the labeling of sensitive proteins and antibodies. The dye is useful for many demanding applications, including microscopy.
From the chemical standpoint, AF488 is a sulfonated rhodamine dye Rhodamine 110 (R110). Like other rhodamines, it is available as 5- and 6-isomers, which have almost identical photophysical properties. The isomers need to be separated though – otherwise, use of mixed isomer dye can lead to doubled peaks during HPLC or electrophoresis separations of the labeled products. This product is an isomerically pure 5-AF488.
This NHS ester is an amine reactive dye, it can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules.
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Absorption and emission spectra of AF488
Recommended protocol
NHS ester labeling of amino biomolecules
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Protein labeling calculator
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