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TAMRA (tetramethylrhodamine) is a xanthene dye of rhodamine series. This fluorophore is being used for quite a long time for the preparation of dual labeled qPCR TaqMan oligonucleotide probes containing TAMRA and fluorescein (FAM).
Just like many other xanthene fluorophores, TAMRA is a available as two isomers (5- and 6-isomer) which have nearly identical optical properties. This product is an isomerically pure 5-TAMRA.
TAMRA NHS is an amine reactive reagent. It can be used for the labeling of proteins, peptides, and modified oligonucleotides containing amine groups.
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Absorption and emission spectra of 5-TAMRA
Recommended protocol
NHS ester labeling of amino biomolecules
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Protein labeling calculator
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