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TCO ligation is a reaction that takes place between a tetrazine and a strained olefin, such as trans-cyclooctene.
This tetrazine bears a fragment of near infrared dye, Cyanine7. This NIR fluorophore is especially useful for in vivo imaging.
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Absorption and emission spectra of Cyanine7
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BDP FL tetrazine
BDP FL tetrazine (methyltetrazine) is a reactive dye for the inverse electron demand Diels-Alder (ied-DA) reaction with trans-cyclooctenes, cyclopropenes, and other strained cycloolefines. BDP FL is a bright and photostable dye for the 488 nm fluorescein (FAM) channel.