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Biotin-11-dCTP is a deoxycytidine triphosphate (dCTP) linked to a biotine molecule via an 11-atom linker, which allows efficient interaction with avidin and streptavidin; incorporation into DNA proceeds as with natural dCTP.
Biotin-11-dCTP is a substrate for Klenow fragment, T4 and Taq DNA-polymerases, reverse transcriptase, and terminal transferase. Biotinylated DNA can be detected using alkaline phosphatase-conjugated avidin/streptavidin or relevant fluorescent-labeled antibodies.
Biotin-labeled DNA/cDNA probes can be used for fluorescent hybridization in situ or microarray gene profiling.
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