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This reagent is designed for the synthesis of oligonucleotides bearing triple bond (alkyne) in the middle of the chain. Alkyne is attached to deoxyuridine fragment, and it does not interfere with the hybridization to the complementary strand. This allows to prepare modified oligonucleotide probes bearing fluorescent dyes, quenchers, and other fragments attached to the middle of the chain, by virtue of copper catalyzed Click chemistry reaction with various azides.
This phosphoramidite is solid, so it is easy to dispense and handle. It is compatible with the standard deblocking conditions.
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