NEBNext Direct® BRCA1/BRCA2 Panel–NEB酶试剂

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产品资料 – 用于二代测序的 NEBNext® 试剂 – 适用于 Illumina 测序平台/靶向富集

NEBNext Direct® BRCA1/BRCA2 Panel                              收藏

货 号
规 格
价 格
北京库存
上海库存
广州库存
成都库存

#E6627L
24 reactions
21,599.00元
无
无
无
无

#E6627S
8 reactions
7,679.00元
无
无
无
无

#E6627X
96 reactions
81,799.00元
无
无
无
无

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产品优势

   Generate full (100%) coverage of all protein coding regions in BRCA1 and BRCA2 genes

   Obtain highly uniform sequencing across exon targets- 100% of base pairs in panel have coverage

       greater than 20% of the mean target coverage

   Maximize efficiency with a 1-day workflow that combines highly specific enrichment with library

      preparation

   Produce high depths of target coverage across a wide range of DNA input amounts for germline and

      somatic variant calling

概述

The NEBNext Direct BRCA1/BRCA2 Panel enriches the complete exon content of BRCA1 and BRCA2

genes for next-generation se­quencing analysis. NEBNext Direct employs a unique hybridization-based

enrichment workflow that hybridizes baits directly to genomic DNA, without the need for upfront library

preparation. The BRCA1/BRCA2 panel demonstrates extremely high specificity and unmatched coverage

uniformity across a wide range of DNA inputs, allowing highly sensitive calling of germline and somatic

variants while maximizing sequencer efficiency.

 

Figure 1. NEBNext Direct Workflow.

NEBNext Direct® BRCA1/BRCA2 Panel--NEB酶试剂

 

NEBNext Direct® BRCA1/BRCA2 Panel--NEB酶试剂

 

NEBNext Direct® BRCA1/BRCA2 Panel--NEB酶试剂

 

试剂盒组份

NEBNext Sample Purification Beads
NEBNext Direct BRCA1/BRCA2 Baits
NEBNext Direct® Bead Prep Buffer
NEBNext Direct® Hybridization Buffer
NEBNext Direct® dA-Tailing Buffer
NEBNext Direct® Adaptor Ligation Buffer
NEBNext Direct® Cleaving Buffer  
NEBNext Direct® Hybridization Wash (HW) 
NEBNext Direct® Bead Wash 1
NEBNext Direct® Bead Wash 2 
NEBNext Direct® 3´ Adaptor  
NEBNext Direct® 5´ UMI Adaptor 
NEBNext Direct® dA-Tailing Enzyme
NEBNext Direct® Ligase  
NEBNext Direct® 5´ Blunting Enzyme Mix 
NEBNext Direct® Cleaving Enzyme Mix 
NEBNext Direct® Q5 Master Mix
NEBNext Direct® FFPE Phosphorylation Enzyme
NEBNext Direct® Streptavidin Beads 4
NEBNext Direct® Hybridization Additive -20
NEBNext Direct® 5´ Blunting Buffer 4
NEBNext Direct® 3´ Blunting Enzyme Mix

NEBNext Direct BRCA1/BRCA2 Resources

  • GRCh37hg19 Bed Files for the NEBNext Direct® BRCA1/BRCA2 Panel
  • GRCh38hg38 Bed Files for the NEBNext Direct® BRCA1/BRCA2 Panel
  • Protocol for NEBNext Direct BRCA1/BRCA2 Panel (E6627)