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Hexachlorofluorescein (HEX) is a hexachlorinated derivate of the fluorescent dye fluorescein. HEX-labeled oligonucleotides are used in PCR and the HEX channel is widely used in multiplex qPCR.
Oligonucleotides with a HEX label can be easily generated using HEX azide and alkyne-containing oligonucleotide via azide-alkyne cycloaddition.
HEX exhibits an excitation peak at 535 nm and an emission peak at 555 in green-yellow area, and it can serve as a substitute for JOE, Alexa Fluor™ 532, BODIPY™ 530/550, VIC™ because of the similar spectral characteristics.
Download in ChemDraw format
General properties
Appearance:
orange solid
Molecular weight:
665.09
Molecular formula:
C24H12N4Cl6O6
Quality control:
NMR 1H, HPLC-MS (95%)
Storage conditions:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
