详细介绍
qiagen 59104 甲基化转化试剂盒EpiTect Bisulfite Kit
亚硫酸氢盐的完全转化和DNA回收，用于甲基化分析
- 简化的流程，获得快速、可靠的结果
- 胞嘧啶完全转化
- 独特的DNA保护体系，用于高度敏感的分析
- 优化的试验方案可处理FFPE组织样本DNA
- 离心柱和高通量96孔板方式
- EpiTect Bisulfite Kit可在6小时内将DNA 序列中非甲基化的胞嘧啶完全转化为尿嘧啶。EpiTect 96 Bisulfite操作时间少于7小时。高灵敏度的方法采用全新的保护机制防止DNA降解，确保即使是1 ng的DNA也可获得灵敏的结果，转化率可高达99%。得益于独特的DNA保护技术，EpiTect Bisulfite转化后DNA高度适用于EpiTect Whole Bisulfitome Kits转化后全基因组扩增。因此可在转化后DNA量较少的情况下确保可靠的扩增。EpiTect Bisulfite Kits包括离心柱或96孔板形式的扩增和脱磺酸基过程，确保快速可靠的实验结果用于下游应用。两个试剂盒都可用于离心机或真空装置，另外，EpiTect Bisulfite Kit可在QIAcube全自动核酸纯化仪上全自动运行。
qiagen 59104 甲基化转化试剂盒EpiTect Bisulfite Kit
Performance
快速得到实验结果
完整的EpiTect离心和真空过程只需6小时，包括从DNA到待分析的亚硫酸氢盐转化后的高纯DNA。EpiTect 96 Bisulfite操作时间少于7小时。传统的亚硫酸氢盐转化过程需要18个小时，且需要大量的操作(参见”快速简单的亚硫酸氢盐转化”和”Time saving procedure”)。EpiTect Bisulfite Kit为成功进行亚硫酸氢盐转化和DNA回收提供所需的全部试剂。
| EpiTect流程 | 传统流程 | |
|---|---|---|
| 反应构建 | 10分钟；预制溶液 | 40分钟；需反应构建 |
| 亚硫酸氢盐转化 | 5小时 | 16小时 |
| 纯化 | 30–45分钟； EpiTect离心柱或孔板 | 120分钟；纯化、脱璜、沉淀和洗涤 |
订购信息：
| EpiTect Bisulfite Kit (48) | 2800 | 59104 |
| EpiTect 96 Bisulfite Kit (2) | 9990 | 59110 |
| EpiTect Plus DNA Bisulfite Kit (48) | 2250 | 59124 |
| EpiTect Whole Bisulfitome Kit (25) | 4560 | 59203 |
| EpiTect MSP Kit (100) | 3600 | 59305 |
| EpiTect MethyLight PCR Kit (200) | 10340 | 59436 |
| EpiTect HRM PCR Kit (100) | 1440 | 59445 |
| EpiTect MethyLight PCR +ROX Kit (200) | 10600 | 59496 |
| EpiTect Control DNA (1000) | 2730 | 59568 |
| EpiTect Control DNA, methylated (100) | 2730 | 59655 |
| EpiTect Control DNA, unmethylated (100) | 2730 | 59665 |
| EpiTect PCR Control DNA Set (100) | 5460 | 59695 |
| EpiTect Fast 96 DNA Bisulfite Kit | 10910 | 59720 |
| EpiTect Fast 96 FFPE Bisulfite Kit | 19310 | 59740 |
| EpiTect Fast Bisulfite Kit (10) | 847 | 59802 |
| EpiTect Fast DNA Bisulfite Kit (50) | 2430 | 59824 |
| EpiTect Fast DNA Bisulfite Kit (200) | 8290 | 59826 |
| EpiTect Fast FFPE Bisulfite Kit (50) | 4240 | 59844 |
| EpiTect Fast LyseAll Bisulfite Kit (50) | 2720 | 59864 |
| EpiTect Fast LyseAll Bisulfite Kit (200) | 9270 | 59866 |
| QIAamp DSP Virus Kit (50),CE | 4520 | 60704 |
| QIAamp DSP DNA Blood Mini Kit (50), CE | 2530 | 61104 |
| QIAamp DSP Virus Spin Kit (50) | 3580 | 61704 |
| MagAttract HMW DNA Kit (48) | 3970 | 67563 |
| DNeasy Plant Maxi Kit (24) | 4590 | 68163 |
| DNeasy Plant Mini Kit (50) | 2750 | 69104 |
| DNeasy Plant Mini Kit (250) | 12310 | 69106 |
| DNeasy 96 Plant Kit (6) | 11590 | 69181 |
| DNeasy Blood & Tissue Kit (50) | 2140 | 69504 |
| DNeasy Blood & Tissue Kit (250) | 8810 | 69506 |
| DNeasy mericon Food Kit (50) | 1990 | 69514 |
| DNeasy mericon 96 QIAcube HT Kit (5) | 11430 | 69571 |
| DNeasy 96 Blood & Tissue Kit (4) | 8780 | 69581 |
| DNeasy 96 Blood & Tissue Kit (12) | 24240 | 69582 |
| TissueLyser Single-Bead Dispenser, 5mm | 3520 | 69965 |
| TissueLyser Single-Bead Dispenser, 7mm | 3520 | 69967 |
| TissueLyser 3 mm Bead Dispenser, 96-well | 10160 | 69973 |
| TissueLyser 5 mm Bead Dispenser, 96-well | 10160 | 69975 |
| TissueLyser LT Adapter, 12-Tube | 17250 | 69980 |
| TissueLyser Adapter Set (2 x 24) | 16270 | 69982 |
| TissueLyser Adapter Set (2 x 96) | 12200 | 69984 |
| RNeasy Protect Animal Blood Kit (50) | 6580 | 73224 |
| Rneasy Microarray Tissue Mini Kit (50) | 5350 | 73304 |
| RNeasy Plus Universal Tissue Mini Kit50 | 5330 | 73404 |
| RNeasy Plus Universal Tissue Midi Kit10 | 2820 | 73442 |
| RNeasy FFPE Kit (50) | 5810 | 73504 |
| RNeasy Micro Kit (50) | 6600 | 74004 |
| RNeasy Plus Micro Kit (50) | 5720 | 74034 |
| RNeasy Mini Kit (50) | 4160 | 74104 |
| RNeasy Mini Kit (250) | 17520 | 74106 |
| RNeasy Mini QIAcube Kit (240) | 19280 | 74116 |
| RNeasy Protect Mini Kit (50) | 5000 | 74124 |
| RNeasy Protect Mini Kit (250) | 21950 | 74126 |
| RNeasy Plus Mini Kit (50) | 4760 | 74134 |
| RNeasy Plus Mini Kit (250) | 20060 | 74136 |
| RNeasy 96 QIAcube HT Kit (5) | 14870 | 74171 |
| RNeasy 96 Kit (4) | 21090 | 74181 |
| RNeasy 96 Kit (12) | 57970 | 74182 |
| Rneasy Plus 96 Kit (12) | 66810 | 74192 |
| RNeasy MinElute Cleanup Kit (50) | 4720 | 74204 |
| RNeasy Protect Saliva Mini Kit (50) | 9920 | 74324 |
| RNeasy FFPE Kit (50) | 5940 | 74404 |
| RNeasy Protect Bacteria Mini Kit (50) | 6570 | 74524 |
| RNeasy Protect Cell Mini Kit (50) | 5000 | 74624 |
| RNeasy Fibrous Tissue Mini Kit (50) | 5420 | 74704 |
| RNeasy Lipid Tissue Mini Kit (50) | 5430 | 74804 |
| RNeasy 96 Universal Tissue Kit (4) | 26280 | 74881 |
| RNeasy Plant Mini Kit (20) | 2090 | 74903 |
| RNeasy Plant Mini Kit (50) | 4860 | 74904 |
| RNeasy Midi Kit (10) | 2070 | 75142 |
| RNeasy Midi Kit (50) | 9230 | 75144 |
| RNeasy Maxi Kit (12) | 4900 | 75162 |
| exoEasy Maxi Kit (20) | 6720 | 76064 |
| RNAlater (50ml) | 1140 | 76104 |